Comprehensive sub-mitochondrial protein map of the parasitic protist Trypanosoma brucei defines critical features of organellar biology
Summary: We have generated a high-confidence mitochondrial proteome (MitoTag) of the Trypanosoma brucei procyclic stage containing 1,239 proteins. For 337 of these, a mitochondrial localization had not been described before. We use the TrypTag dataset as a foundation and take advantage of the proper...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-09-01
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| Series: | Cell Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S221112472301094X |
| _version_ | 1797692037094440960 |
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| author | Jan Pyrih Michael Hammond Aline Alves Samuel Dean Jack Daniel Sunter Richard John Wheeler Keith Gull Julius Lukeš |
| author_facet | Jan Pyrih Michael Hammond Aline Alves Samuel Dean Jack Daniel Sunter Richard John Wheeler Keith Gull Julius Lukeš |
| author_sort | Jan Pyrih |
| collection | DOAJ |
| description | Summary: We have generated a high-confidence mitochondrial proteome (MitoTag) of the Trypanosoma brucei procyclic stage containing 1,239 proteins. For 337 of these, a mitochondrial localization had not been described before. We use the TrypTag dataset as a foundation and take advantage of the properties of the fluorescent protein tag that causes aberrant but fortuitous accumulation of tagged matrix and inner membrane proteins near the kinetoplast (mitochondrial DNA). Combined with transmembrane domain predictions, this characteristic allowed categorization of 1,053 proteins into mitochondrial sub-compartments, the detection of unique matrix-localized fucose and methionine synthesis, and the identification of new kinetoplast proteins, which showed kinetoplast-linked pyrimidine synthesis. Moreover, disruption of targeting signals by tagging allowed mapping of the mode of protein targeting to these sub-compartments, identifying a set of C-tail anchored outer mitochondrial membrane proteins and mitochondrial carriers likely employing multiple target peptides. This dataset represents a comprehensive, updated mapping of the mitochondrion. |
| first_indexed | 2024-03-12T02:21:53Z |
| format | Article |
| id | doaj.art-2e86302848f347efa7ac73caef661af2 |
| institution | Directory Open Access Journal |
| issn | 2211-1247 |
| language | English |
| last_indexed | 2024-03-12T02:21:53Z |
| publishDate | 2023-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Cell Reports |
| spelling | doaj.art-2e86302848f347efa7ac73caef661af22023-09-06T04:51:03ZengElsevierCell Reports2211-12472023-09-01429113083Comprehensive sub-mitochondrial protein map of the parasitic protist Trypanosoma brucei defines critical features of organellar biologyJan Pyrih0Michael Hammond1Aline Alves2Samuel Dean3Jack Daniel Sunter4Richard John Wheeler5Keith Gull6Julius Lukeš7Institute of Parasitology, Biology Centre, Czech Academy of Sciences, České Budějovice (Budweis), Czech Republic; Department of Biochemistry, University of Cambridge, Cambridge, UK; Faculty of Science, University of Ostrava, Ostrava, Czech Republic; Corresponding authorInstitute of Parasitology, Biology Centre, Czech Academy of Sciences, České Budějovice (Budweis), Czech RepublicOxford Brookes University, Oxford, UKDivision of Biomedical Sciences, Warwick Medical School, University of Warwick, Coventry, UKOxford Brookes University, Oxford, UKPeter Medawar Building for Pathogen Research, Nuffield Department of Medicine, University of Oxford, Oxford, UKSir William Dunn School of Pathology, University of Oxford, Oxford, UKInstitute of Parasitology, Biology Centre, Czech Academy of Sciences, České Budějovice (Budweis), Czech Republic; Faculty of Sciences, University of South Bohemia, České Budějovice (Budweis), Czech Republic; Corresponding authorSummary: We have generated a high-confidence mitochondrial proteome (MitoTag) of the Trypanosoma brucei procyclic stage containing 1,239 proteins. For 337 of these, a mitochondrial localization had not been described before. We use the TrypTag dataset as a foundation and take advantage of the properties of the fluorescent protein tag that causes aberrant but fortuitous accumulation of tagged matrix and inner membrane proteins near the kinetoplast (mitochondrial DNA). Combined with transmembrane domain predictions, this characteristic allowed categorization of 1,053 proteins into mitochondrial sub-compartments, the detection of unique matrix-localized fucose and methionine synthesis, and the identification of new kinetoplast proteins, which showed kinetoplast-linked pyrimidine synthesis. Moreover, disruption of targeting signals by tagging allowed mapping of the mode of protein targeting to these sub-compartments, identifying a set of C-tail anchored outer mitochondrial membrane proteins and mitochondrial carriers likely employing multiple target peptides. This dataset represents a comprehensive, updated mapping of the mitochondrion.http://www.sciencedirect.com/science/article/pii/S221112472301094XCP: Cell biology |
| spellingShingle | Jan Pyrih Michael Hammond Aline Alves Samuel Dean Jack Daniel Sunter Richard John Wheeler Keith Gull Julius Lukeš Comprehensive sub-mitochondrial protein map of the parasitic protist Trypanosoma brucei defines critical features of organellar biology Cell Reports CP: Cell biology |
| title | Comprehensive sub-mitochondrial protein map of the parasitic protist Trypanosoma brucei defines critical features of organellar biology |
| title_full | Comprehensive sub-mitochondrial protein map of the parasitic protist Trypanosoma brucei defines critical features of organellar biology |
| title_fullStr | Comprehensive sub-mitochondrial protein map of the parasitic protist Trypanosoma brucei defines critical features of organellar biology |
| title_full_unstemmed | Comprehensive sub-mitochondrial protein map of the parasitic protist Trypanosoma brucei defines critical features of organellar biology |
| title_short | Comprehensive sub-mitochondrial protein map of the parasitic protist Trypanosoma brucei defines critical features of organellar biology |
| title_sort | comprehensive sub mitochondrial protein map of the parasitic protist trypanosoma brucei defines critical features of organellar biology |
| topic | CP: Cell biology |
| url | http://www.sciencedirect.com/science/article/pii/S221112472301094X |
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