Reticulocyte Antioxidant Enzymes mRNA Levels versus Reticulocyte Maturity Indices in Hereditary Spherocytosis, β-Thalassemia and Sickle Cell Disease
The antioxidant enzymes superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and peroxiredoxin 2 (Prx2) are particularly important in erythroid cells. Reticulocytes and other erythroid precursors may adapt their biosynthetic mechanisms to cell defects or to changes in the bone ma...
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MDPI AG
2024-02-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/25/4/2159 |
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| author | Daniela Melo Fátima Ferreira Maria José Teles Graça Porto Susana Coimbra Susana Rocha Alice Santos-Silva |
| author_facet | Daniela Melo Fátima Ferreira Maria José Teles Graça Porto Susana Coimbra Susana Rocha Alice Santos-Silva |
| author_sort | Daniela Melo |
| collection | DOAJ |
| description | The antioxidant enzymes superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and peroxiredoxin 2 (Prx2) are particularly important in erythroid cells. Reticulocytes and other erythroid precursors may adapt their biosynthetic mechanisms to cell defects or to changes in the bone marrow environment. Our aim was to perform a comparative study of the mRNA levels of <i>CAT, GPX1, PRDX2</i> and <i>SOD1</i> in reticulocytes from healthy individuals and from patients with hereditary spherocytosis (HS), sickle cell disease (SCD) and β-thalassemia (β-thal), and to study the association between their transcript levels and the reticulocyte maturity indices. In controls, the enzyme mRNA levels were significantly correlated with reticulocyte maturity indices for all genes except for <i>SOD1</i>. HS, SCD and β-thal patients showed younger reticulocytes, with higher transcript levels of all enzymes, although with different patterns. β-thal and HS showed similar reticulocyte maturity, with different enzyme mRNA levels; SCD and HS, with different reticulocyte maturity, presented similar enzyme mRNA levels. Our data suggest that the transcript profile for these antioxidant enzymes is not entirely related to reticulocyte maturity; it appears to also reflect adaptive mechanisms to abnormal erythropoiesis and/or to altered erythropoietic environments, leading to reticulocytes with distinct antioxidant potential according to each anemia. |
| first_indexed | 2024-03-07T22:29:27Z |
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| id | doaj.art-2e8e6048b4cc44989bb5adde0d78f00e |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-07T22:29:27Z |
| publishDate | 2024-02-01 |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-2e8e6048b4cc44989bb5adde0d78f00e2024-02-23T15:19:58ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672024-02-01254215910.3390/ijms25042159Reticulocyte Antioxidant Enzymes mRNA Levels versus Reticulocyte Maturity Indices in Hereditary Spherocytosis, β-Thalassemia and Sickle Cell DiseaseDaniela Melo0Fátima Ferreira1Maria José Teles2Graça Porto3Susana Coimbra4Susana Rocha5Alice Santos-Silva6UCIBIO—Applied Molecular Biosciences Unit, Laboratory of Biochemistry, Department of Biological Sciences, Faculty of Pharmacy, University of Porto, 4051-401 Porto, PortugalHematology Service, Centro Hospitalar e Universitário de São João, 4051-401 Porto, PortugalClinical Pathology, Centro Hospitalar e Universitário de São João, 4051-401 Porto, PortugalImuno-Hemotherapy Service, Centro Hospitalar Universitário de Santo António, 4051-401 Porto, PortugalUCIBIO—Applied Molecular Biosciences Unit, Laboratory of Biochemistry, Department of Biological Sciences, Faculty of Pharmacy, University of Porto, 4051-401 Porto, PortugalUCIBIO—Applied Molecular Biosciences Unit, Laboratory of Biochemistry, Department of Biological Sciences, Faculty of Pharmacy, University of Porto, 4051-401 Porto, PortugalUCIBIO—Applied Molecular Biosciences Unit, Laboratory of Biochemistry, Department of Biological Sciences, Faculty of Pharmacy, University of Porto, 4051-401 Porto, PortugalThe antioxidant enzymes superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and peroxiredoxin 2 (Prx2) are particularly important in erythroid cells. Reticulocytes and other erythroid precursors may adapt their biosynthetic mechanisms to cell defects or to changes in the bone marrow environment. Our aim was to perform a comparative study of the mRNA levels of <i>CAT, GPX1, PRDX2</i> and <i>SOD1</i> in reticulocytes from healthy individuals and from patients with hereditary spherocytosis (HS), sickle cell disease (SCD) and β-thalassemia (β-thal), and to study the association between their transcript levels and the reticulocyte maturity indices. In controls, the enzyme mRNA levels were significantly correlated with reticulocyte maturity indices for all genes except for <i>SOD1</i>. HS, SCD and β-thal patients showed younger reticulocytes, with higher transcript levels of all enzymes, although with different patterns. β-thal and HS showed similar reticulocyte maturity, with different enzyme mRNA levels; SCD and HS, with different reticulocyte maturity, presented similar enzyme mRNA levels. Our data suggest that the transcript profile for these antioxidant enzymes is not entirely related to reticulocyte maturity; it appears to also reflect adaptive mechanisms to abnormal erythropoiesis and/or to altered erythropoietic environments, leading to reticulocytes with distinct antioxidant potential according to each anemia.https://www.mdpi.com/1422-0067/25/4/2159reticulocytehereditary spherocytosisβ-thalassemiasickle cell diseaseantioxidant enzymes |
| spellingShingle | Daniela Melo Fátima Ferreira Maria José Teles Graça Porto Susana Coimbra Susana Rocha Alice Santos-Silva Reticulocyte Antioxidant Enzymes mRNA Levels versus Reticulocyte Maturity Indices in Hereditary Spherocytosis, β-Thalassemia and Sickle Cell Disease International Journal of Molecular Sciences reticulocyte hereditary spherocytosis β-thalassemia sickle cell disease antioxidant enzymes |
| title | Reticulocyte Antioxidant Enzymes mRNA Levels versus Reticulocyte Maturity Indices in Hereditary Spherocytosis, β-Thalassemia and Sickle Cell Disease |
| title_full | Reticulocyte Antioxidant Enzymes mRNA Levels versus Reticulocyte Maturity Indices in Hereditary Spherocytosis, β-Thalassemia and Sickle Cell Disease |
| title_fullStr | Reticulocyte Antioxidant Enzymes mRNA Levels versus Reticulocyte Maturity Indices in Hereditary Spherocytosis, β-Thalassemia and Sickle Cell Disease |
| title_full_unstemmed | Reticulocyte Antioxidant Enzymes mRNA Levels versus Reticulocyte Maturity Indices in Hereditary Spherocytosis, β-Thalassemia and Sickle Cell Disease |
| title_short | Reticulocyte Antioxidant Enzymes mRNA Levels versus Reticulocyte Maturity Indices in Hereditary Spherocytosis, β-Thalassemia and Sickle Cell Disease |
| title_sort | reticulocyte antioxidant enzymes mrna levels versus reticulocyte maturity indices in hereditary spherocytosis β thalassemia and sickle cell disease |
| topic | reticulocyte hereditary spherocytosis β-thalassemia sickle cell disease antioxidant enzymes |
| url | https://www.mdpi.com/1422-0067/25/4/2159 |
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