Blood Clotting Dissolution in the Presence of a Magnetic Field and Preliminary Study with MG63 Osteoblast-like Cells—Further Developments for Guided Bone Regeneration?
Background: The influence of a magnetic field on the activation of bone cells and remodelling of alveolar bone is known to incite bone regeneration. Guided Bone Regeneration (GBR) aims to develop biomimetic scaffolds to allow for the functioning of the barrier and the precise succession of wound hea...
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| Format: | Article |
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MDPI AG
2023-07-01
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| Series: | Bioengineering |
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| Online Access: | https://www.mdpi.com/2306-5354/10/8/888 |
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| author | Sante Di Gioia Lucio Milillo Md Niamat Hossain Annalucia Carbone Massimo Petruzzi Massimo Conese |
| author_facet | Sante Di Gioia Lucio Milillo Md Niamat Hossain Annalucia Carbone Massimo Petruzzi Massimo Conese |
| author_sort | Sante Di Gioia |
| collection | DOAJ |
| description | Background: The influence of a magnetic field on the activation of bone cells and remodelling of alveolar bone is known to incite bone regeneration. Guided Bone Regeneration (GBR) aims to develop biomimetic scaffolds to allow for the functioning of the barrier and the precise succession of wound healing steps, including haemostasis. The effect of a magnetic field on blood clot dissolution has not been studied yet. Methods: We conducted a methodological study on the clot stability in the presence of a static magnetic field (SMF). Preformed whole blood (WB) clots were treated with either a broad proteolytic enzyme (trypsin) or a specific fibrinolytic agent, i.e., tissue-type plasminogen activator (t-PA). MG63 osteoblast-like cells were added to preformed WB clots to assess cell proliferation. Results: After having experienced a number of clotting and dissolution protocols, we obtained clot stability exerted by SMF when tissue factor (for clotting) and t-PA + plasminogen (for fibrinolysis) were used. WB clots allowed osteoblast-like cells to survive and proliferate, however no obvious effects of the magnetic field were noted. Conclusions: Paramagnetic properties of erythrocytes may have influenced the reduction in clot dissolution. Future studies are warranted to fully exploit the combination of magnetic forces, WB clot and cells in GBR applied to orthodontics and prosthodontics. |
| first_indexed | 2024-03-11T00:07:13Z |
| format | Article |
| id | doaj.art-2e90fefdc2f2415f8234705e85499924 |
| institution | Directory Open Access Journal |
| issn | 2306-5354 |
| language | English |
| last_indexed | 2024-03-11T00:07:13Z |
| publishDate | 2023-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Bioengineering |
| spelling | doaj.art-2e90fefdc2f2415f8234705e854999242023-11-19T00:17:27ZengMDPI AGBioengineering2306-53542023-07-0110888810.3390/bioengineering10080888Blood Clotting Dissolution in the Presence of a Magnetic Field and Preliminary Study with MG63 Osteoblast-like Cells—Further Developments for Guided Bone Regeneration?Sante Di Gioia0Lucio Milillo1Md Niamat Hossain2Annalucia Carbone3Massimo Petruzzi4Massimo Conese5Department of Clinical and Experimental Medicine, University of Foggia, 71122 Foggia, ItalyIndependent Researcher, 70126 Bari, ItalyDepartment of Clinical and Experimental Medicine, University of Foggia, 71122 Foggia, ItalyDepartment of Clinical and Experimental Medicine, University of Foggia, 71122 Foggia, ItalyDepartment of Interdisciplinary Medicine, University of Bari “Aldo Moro”, 70126 Bari, ItalyDepartment of Clinical and Experimental Medicine, University of Foggia, 71122 Foggia, ItalyBackground: The influence of a magnetic field on the activation of bone cells and remodelling of alveolar bone is known to incite bone regeneration. Guided Bone Regeneration (GBR) aims to develop biomimetic scaffolds to allow for the functioning of the barrier and the precise succession of wound healing steps, including haemostasis. The effect of a magnetic field on blood clot dissolution has not been studied yet. Methods: We conducted a methodological study on the clot stability in the presence of a static magnetic field (SMF). Preformed whole blood (WB) clots were treated with either a broad proteolytic enzyme (trypsin) or a specific fibrinolytic agent, i.e., tissue-type plasminogen activator (t-PA). MG63 osteoblast-like cells were added to preformed WB clots to assess cell proliferation. Results: After having experienced a number of clotting and dissolution protocols, we obtained clot stability exerted by SMF when tissue factor (for clotting) and t-PA + plasminogen (for fibrinolysis) were used. WB clots allowed osteoblast-like cells to survive and proliferate, however no obvious effects of the magnetic field were noted. Conclusions: Paramagnetic properties of erythrocytes may have influenced the reduction in clot dissolution. Future studies are warranted to fully exploit the combination of magnetic forces, WB clot and cells in GBR applied to orthodontics and prosthodontics.https://www.mdpi.com/2306-5354/10/8/888guided bone regenerationclot stabilitystatic magnetic fieldtrypsinfibrinolysistissue-type plasminogen activator |
| spellingShingle | Sante Di Gioia Lucio Milillo Md Niamat Hossain Annalucia Carbone Massimo Petruzzi Massimo Conese Blood Clotting Dissolution in the Presence of a Magnetic Field and Preliminary Study with MG63 Osteoblast-like Cells—Further Developments for Guided Bone Regeneration? Bioengineering guided bone regeneration clot stability static magnetic field trypsin fibrinolysis tissue-type plasminogen activator |
| title | Blood Clotting Dissolution in the Presence of a Magnetic Field and Preliminary Study with MG63 Osteoblast-like Cells—Further Developments for Guided Bone Regeneration? |
| title_full | Blood Clotting Dissolution in the Presence of a Magnetic Field and Preliminary Study with MG63 Osteoblast-like Cells—Further Developments for Guided Bone Regeneration? |
| title_fullStr | Blood Clotting Dissolution in the Presence of a Magnetic Field and Preliminary Study with MG63 Osteoblast-like Cells—Further Developments for Guided Bone Regeneration? |
| title_full_unstemmed | Blood Clotting Dissolution in the Presence of a Magnetic Field and Preliminary Study with MG63 Osteoblast-like Cells—Further Developments for Guided Bone Regeneration? |
| title_short | Blood Clotting Dissolution in the Presence of a Magnetic Field and Preliminary Study with MG63 Osteoblast-like Cells—Further Developments for Guided Bone Regeneration? |
| title_sort | blood clotting dissolution in the presence of a magnetic field and preliminary study with mg63 osteoblast like cells further developments for guided bone regeneration |
| topic | guided bone regeneration clot stability static magnetic field trypsin fibrinolysis tissue-type plasminogen activator |
| url | https://www.mdpi.com/2306-5354/10/8/888 |
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