Involvement of Estrogen and Its Receptors in Morphological Changes in the Eyes of the Japanese Eel, <i>Anguilla japonica</i>, in the Process of Artificially-Induced Maturation
During the long migration from river habitats to the spawning ground, the Japanese eel undergoes sexual maturation. This spawning migration occurs concurrently with morphological changes, such as increases in eye size; however, the mechanisms by which sex steroids and their receptors influence these...
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MDPI AG
2019-04-01
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| Series: | Cells |
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| Online Access: | https://www.mdpi.com/2073-4409/8/4/310 |
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| author | Ji-Yeon Hyeon Sung-Pyo Hur Byeong-Hoon Kim Jun-Hwan Byun Eun-Su Kim Bong-Soo Lim Bae-Ik Lee Shin-Kwon Kim Akihiro Takemura Se-Jae Kim |
| author_facet | Ji-Yeon Hyeon Sung-Pyo Hur Byeong-Hoon Kim Jun-Hwan Byun Eun-Su Kim Bong-Soo Lim Bae-Ik Lee Shin-Kwon Kim Akihiro Takemura Se-Jae Kim |
| author_sort | Ji-Yeon Hyeon |
| collection | DOAJ |
| description | During the long migration from river habitats to the spawning ground, the Japanese eel undergoes sexual maturation. This spawning migration occurs concurrently with morphological changes, such as increases in eye size; however, the mechanisms by which sex steroids and their receptors influence these changes in peripheral tissues remain unclear. The aim of this study was to investigate changes in the eyes of female Japanese eels during sexual maturation, and our research focused on estrogen receptor (ER)α and ERβ transcripts. During ovarian development, the gonadosomatic index increased and yolk-laden oocytes developed rapidly. These changes occurred in conjunction with a steady increase in plasma levels of estradiol-17β (E<sub>2</sub>). Concomitant increases in transcript levels of ERα and ERβ in eye, brain, pituitary, and ovary were also observed. Fluorescence in-situ hybridization analyses revealed that ERα and ERβ transcripts were present in the choriocapillary layer and photoreceptor layer of the eyes, and the analysis also revealed that their signals in these layers became stronger in mature females compared to those observed in immature females, suggesting that under the influence of gonadotropins, morphological changes in the eyes are regulated by E<sub>2</sub> through the activation of its receptors. In conclusion, E<sub>2</sub> plays a crucial role in physiological adaptations that occur in peripheral tissues during the spawning migration. |
| first_indexed | 2024-03-12T05:47:16Z |
| format | Article |
| id | doaj.art-2e91f59e0ed44598a3fd493bc15b0576 |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-12T05:47:16Z |
| publishDate | 2019-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-2e91f59e0ed44598a3fd493bc15b05762023-09-03T05:32:19ZengMDPI AGCells2073-44092019-04-018431010.3390/cells8040310cells8040310Involvement of Estrogen and Its Receptors in Morphological Changes in the Eyes of the Japanese Eel, <i>Anguilla japonica</i>, in the Process of Artificially-Induced MaturationJi-Yeon Hyeon0Sung-Pyo Hur1Byeong-Hoon Kim2Jun-Hwan Byun3Eun-Su Kim4Bong-Soo Lim5Bae-Ik Lee6Shin-Kwon Kim7Akihiro Takemura8Se-Jae Kim9Jeju International Marine Science Research & Logistics Center, Korea Institute of Ocean Science & Technology, 2670 Iljudong-ro, Gujwa, Jeju 63349, KoreaJeju International Marine Science Research & Logistics Center, Korea Institute of Ocean Science & Technology, 2670 Iljudong-ro, Gujwa, Jeju 63349, KoreaMarine Science Institute, Jeju National University, 19-5 Hamdeok 5-gil, Jocheon, Jeju 63333, KoreaDepartment of Chemistry, Biology and Marine Science, Faculty of Science, University of the Ryukyus, 1 Senbaru, Nishihara, Okinawa 903-0213, JapanSolforto Co. Ltd., 19 Yeondong 8-gil, Jeju 63133, KoreaSolforto Co. Ltd., 19 Yeondong 8-gil, Jeju 63133, KoreaAquaculture Research Division, National Institute of Fisheries Science, 216 Gijanghaean-ro, Gijang, Busan 46083, KoreaAquaculture Research Division, National Institute of Fisheries Science, 216 Gijanghaean-ro, Gijang, Busan 46083, KoreaDepartment of Chemistry, Biology and Marine Science, Faculty of Science, University of the Ryukyus, 1 Senbaru, Nishihara, Okinawa 903-0213, JapanDepartment of Biology, Jeju National University, 102 Jejudaehak-ro, Jeju 63243, KoreaDuring the long migration from river habitats to the spawning ground, the Japanese eel undergoes sexual maturation. This spawning migration occurs concurrently with morphological changes, such as increases in eye size; however, the mechanisms by which sex steroids and their receptors influence these changes in peripheral tissues remain unclear. The aim of this study was to investigate changes in the eyes of female Japanese eels during sexual maturation, and our research focused on estrogen receptor (ER)α and ERβ transcripts. During ovarian development, the gonadosomatic index increased and yolk-laden oocytes developed rapidly. These changes occurred in conjunction with a steady increase in plasma levels of estradiol-17β (E<sub>2</sub>). Concomitant increases in transcript levels of ERα and ERβ in eye, brain, pituitary, and ovary were also observed. Fluorescence in-situ hybridization analyses revealed that ERα and ERβ transcripts were present in the choriocapillary layer and photoreceptor layer of the eyes, and the analysis also revealed that their signals in these layers became stronger in mature females compared to those observed in immature females, suggesting that under the influence of gonadotropins, morphological changes in the eyes are regulated by E<sub>2</sub> through the activation of its receptors. In conclusion, E<sub>2</sub> plays a crucial role in physiological adaptations that occur in peripheral tissues during the spawning migration.https://www.mdpi.com/2073-4409/8/4/310Japanese eel<i>Anguilla japonica</i>estrogen receptorestradiol-17βovarian developmenteye sizechoriocapillary layereel spawning |
| spellingShingle | Ji-Yeon Hyeon Sung-Pyo Hur Byeong-Hoon Kim Jun-Hwan Byun Eun-Su Kim Bong-Soo Lim Bae-Ik Lee Shin-Kwon Kim Akihiro Takemura Se-Jae Kim Involvement of Estrogen and Its Receptors in Morphological Changes in the Eyes of the Japanese Eel, <i>Anguilla japonica</i>, in the Process of Artificially-Induced Maturation Cells Japanese eel <i>Anguilla japonica</i> estrogen receptor estradiol-17β ovarian development eye size choriocapillary layer eel spawning |
| title | Involvement of Estrogen and Its Receptors in Morphological Changes in the Eyes of the Japanese Eel, <i>Anguilla japonica</i>, in the Process of Artificially-Induced Maturation |
| title_full | Involvement of Estrogen and Its Receptors in Morphological Changes in the Eyes of the Japanese Eel, <i>Anguilla japonica</i>, in the Process of Artificially-Induced Maturation |
| title_fullStr | Involvement of Estrogen and Its Receptors in Morphological Changes in the Eyes of the Japanese Eel, <i>Anguilla japonica</i>, in the Process of Artificially-Induced Maturation |
| title_full_unstemmed | Involvement of Estrogen and Its Receptors in Morphological Changes in the Eyes of the Japanese Eel, <i>Anguilla japonica</i>, in the Process of Artificially-Induced Maturation |
| title_short | Involvement of Estrogen and Its Receptors in Morphological Changes in the Eyes of the Japanese Eel, <i>Anguilla japonica</i>, in the Process of Artificially-Induced Maturation |
| title_sort | involvement of estrogen and its receptors in morphological changes in the eyes of the japanese eel i anguilla japonica i in the process of artificially induced maturation |
| topic | Japanese eel <i>Anguilla japonica</i> estrogen receptor estradiol-17β ovarian development eye size choriocapillary layer eel spawning |
| url | https://www.mdpi.com/2073-4409/8/4/310 |
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