G-protein β subunit AGB1 positively regulates salt stress tolerance in Arabidopsis
The heterotrimeric GTP-binding proteins (G-proteins) in eukaryotes consisted of α, β and γ subunits and are important in molecular signaling by interacting with G-protein-coupled receptors (GPCR), on which to transduce signaling into the cytoplast through appropriate downstream effectors. However, d...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2015-02-01
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| Series: | Journal of Integrative Agriculture |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2095311914607772 |
| _version_ | 1818839042795503616 |
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| author | Ya-nan MA Ming CHEN Dong-bei XU Guang-ning FANG Er-hui WANG Shi-qing GAO Zhao-shi XU Lian-cheng LI Xiao-hong ZHANG Dong-hong MIN You-zhi MA |
| author_facet | Ya-nan MA Ming CHEN Dong-bei XU Guang-ning FANG Er-hui WANG Shi-qing GAO Zhao-shi XU Lian-cheng LI Xiao-hong ZHANG Dong-hong MIN You-zhi MA |
| author_sort | Ya-nan MA |
| collection | DOAJ |
| description | The heterotrimeric GTP-binding proteins (G-proteins) in eukaryotes consisted of α, β and γ subunits and are important in molecular signaling by interacting with G-protein-coupled receptors (GPCR), on which to transduce signaling into the cytoplast through appropriate downstream effectors. However, downstream effectors regulated by the G-proteins in plants are currently not well defined. In this study, the transcripts of AGB1, a G protein β subunit gene in Arabidopsis were found to be down-regulated by cold and heat, but up-regulated by high salt stress treatment. AGB1 mutant (agb1-2) was more sensitive to high salt stress than wild-type (WT). Compared with WT, the cotyledon greening rates, fresh weight, root length, seedling germination rates and survival rates decreased more rapidly in agb1-2 along with increasing concentrations of NaCl in normal (MS) medium. Physiological characteristic analysis showed that compared to WT, the contents of chlorophyll, relative proline accumulation and peroxidase (POD) were reduced, whereas the malonaldehyde (MDA) content and concentration ratio of Na+/K+ were increased in agb1-2 under salt stress condition. Further studies on the expression of several stress inducible genes associated with above physiological processes were investigated, and the results revealed that the expressions of genes related to proline biosynthesis, oxidative stress response, Na+ homeostasis, stress- and ABA-responses were lower in agb1-2 than in WT, suggesting that those genes are possible downstream genes of AGB1 and that their changed expression plays an important role in determining phenotypic and physiologic traits in agb1-2. Taken together, these findings indicate that AGB1 positively regulates salt tolerance in Arabidopsis through its modulation of genes transcription related to proline biosynthesis, oxidative stress, ion homeostasis, stress- and ABA-responses. |
| first_indexed | 2024-12-19T03:48:00Z |
| format | Article |
| id | doaj.art-2e93df3fbf8647a188ab0f7dd71fa97a |
| institution | Directory Open Access Journal |
| issn | 2095-3119 |
| language | English |
| last_indexed | 2024-12-19T03:48:00Z |
| publishDate | 2015-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Integrative Agriculture |
| spelling | doaj.art-2e93df3fbf8647a188ab0f7dd71fa97a2022-12-21T20:37:04ZengElsevierJournal of Integrative Agriculture2095-31192015-02-01142314325G-protein β subunit AGB1 positively regulates salt stress tolerance in ArabidopsisYa-nan MA0Ming CHEN1Dong-bei XU2Guang-ning FANG3Er-hui WANG4Shi-qing GAO5Zhao-shi XU6Lian-cheng LI7Xiao-hong ZHANG8Dong-hong MIN9You-zhi MA10College of Agronomy, Northwest A&F University/State Key Laboratory of Arid Region Crop Adversity Biology, Yangling 712100, P.R.China; Institute of Crop Sciences, Chinese Academy of Agricultural Sciences/National Key Facility for Crop Gene Resources and Genetic Improvement/Key Laboratory of Biology and Genetic Improvement of Triticeae Crops, Ministry of Agriculture, Beijing 100081, P.R.ChinaInstitute of Crop Sciences, Chinese Academy of Agricultural Sciences/National Key Facility for Crop Gene Resources and Genetic Improvement/Key Laboratory of Biology and Genetic Improvement of Triticeae Crops, Ministry of Agriculture, Beijing 100081, P.R.ChinaCollege of Agronomy, Northwest A&F University/State Key Laboratory of Arid Region Crop Adversity Biology, Yangling 712100, P.R.ChinaCollege of Agronomy, Northwest A&F University/State Key Laboratory of Arid Region Crop Adversity Biology, Yangling 712100, P.R.ChinaCollege of Agronomy, Northwest A&F University/State Key Laboratory of Arid Region Crop Adversity Biology, Yangling 712100, P.R.ChinaBeijing Hybrid Wheat Engineering and Technology Research Center, Beijing 100097, P.R.ChinaInstitute of Crop Sciences, Chinese Academy of Agricultural Sciences/National Key Facility for Crop Gene Resources and Genetic Improvement/Key Laboratory of Biology and Genetic Improvement of Triticeae Crops, Ministry of Agriculture, Beijing 100081, P.R.ChinaInstitute of Crop Sciences, Chinese Academy of Agricultural Sciences/National Key Facility for Crop Gene Resources and Genetic Improvement/Key Laboratory of Biology and Genetic Improvement of Triticeae Crops, Ministry of Agriculture, Beijing 100081, P.R.ChinaCollege of Agronomy, Northwest A&F University/State Key Laboratory of Arid Region Crop Adversity Biology, Yangling 712100, P.R.ChinaCollege of Agronomy, Northwest A&F University/State Key Laboratory of Arid Region Crop Adversity Biology, Yangling 712100, P.R.China; Correspondence MIN Dong-hongInstitute of Crop Sciences, Chinese Academy of Agricultural Sciences/National Key Facility for Crop Gene Resources and Genetic Improvement/Key Laboratory of Biology and Genetic Improvement of Triticeae Crops, Ministry of Agriculture, Beijing 100081, P.R.ChinaThe heterotrimeric GTP-binding proteins (G-proteins) in eukaryotes consisted of α, β and γ subunits and are important in molecular signaling by interacting with G-protein-coupled receptors (GPCR), on which to transduce signaling into the cytoplast through appropriate downstream effectors. However, downstream effectors regulated by the G-proteins in plants are currently not well defined. In this study, the transcripts of AGB1, a G protein β subunit gene in Arabidopsis were found to be down-regulated by cold and heat, but up-regulated by high salt stress treatment. AGB1 mutant (agb1-2) was more sensitive to high salt stress than wild-type (WT). Compared with WT, the cotyledon greening rates, fresh weight, root length, seedling germination rates and survival rates decreased more rapidly in agb1-2 along with increasing concentrations of NaCl in normal (MS) medium. Physiological characteristic analysis showed that compared to WT, the contents of chlorophyll, relative proline accumulation and peroxidase (POD) were reduced, whereas the malonaldehyde (MDA) content and concentration ratio of Na+/K+ were increased in agb1-2 under salt stress condition. Further studies on the expression of several stress inducible genes associated with above physiological processes were investigated, and the results revealed that the expressions of genes related to proline biosynthesis, oxidative stress response, Na+ homeostasis, stress- and ABA-responses were lower in agb1-2 than in WT, suggesting that those genes are possible downstream genes of AGB1 and that their changed expression plays an important role in determining phenotypic and physiologic traits in agb1-2. Taken together, these findings indicate that AGB1 positively regulates salt tolerance in Arabidopsis through its modulation of genes transcription related to proline biosynthesis, oxidative stress, ion homeostasis, stress- and ABA-responses.http://www.sciencedirect.com/science/article/pii/S2095311914607772Arabidopsisheterotrimeric G-protein β subunitphysiological processessalt stress tolerance |
| spellingShingle | Ya-nan MA Ming CHEN Dong-bei XU Guang-ning FANG Er-hui WANG Shi-qing GAO Zhao-shi XU Lian-cheng LI Xiao-hong ZHANG Dong-hong MIN You-zhi MA G-protein β subunit AGB1 positively regulates salt stress tolerance in Arabidopsis Journal of Integrative Agriculture Arabidopsis heterotrimeric G-protein β subunit physiological processes salt stress tolerance |
| title | G-protein β subunit AGB1 positively regulates salt stress tolerance in Arabidopsis |
| title_full | G-protein β subunit AGB1 positively regulates salt stress tolerance in Arabidopsis |
| title_fullStr | G-protein β subunit AGB1 positively regulates salt stress tolerance in Arabidopsis |
| title_full_unstemmed | G-protein β subunit AGB1 positively regulates salt stress tolerance in Arabidopsis |
| title_short | G-protein β subunit AGB1 positively regulates salt stress tolerance in Arabidopsis |
| title_sort | g protein β subunit agb1 positively regulates salt stress tolerance in arabidopsis |
| topic | Arabidopsis heterotrimeric G-protein β subunit physiological processes salt stress tolerance |
| url | http://www.sciencedirect.com/science/article/pii/S2095311914607772 |
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