Mechanism of SOS PR-domain autoinhibition revealed by single-molecule assays on native protein from lysate
The guanine nucleotide exchange factor SOS is a critical intermediary that transduces receptor tyrosine kinase stimulation into Ras activation. Here the authors develop a single molecule assay in which SOS is captured from raw lysates using Ras-functionalized membrane microarrays and uncover an auto...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2017-04-01
|
| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/ncomms15061 |