FocVel1 influences asexual production, filamentous growth, biofilm formation, and virulence in Fusarium oxysporum f. sp. cucumerinum
Velvet genes play critical roles in the regulation of diverse cellular processes. In current study, we identified the gene FocVel1, a homolog of Fusarium graminearum VelA, in the plant pathogenic fungus F. oxysporum f. sp. cucumerinum. This pathogen causes the destructive disease called cucumber Fus...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2015-05-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fpls.2015.00312/full |
| _version_ | 1818360807303413760 |
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| author | Li ePeiqian Li ePeiqian Li ePeiqian Pu eXiaoming Feng eBaozhen Yang eQiyun Shen eHuifang Jang eZingxin Lin eBirun |
| author_facet | Li ePeiqian Li ePeiqian Li ePeiqian Pu eXiaoming Feng eBaozhen Yang eQiyun Shen eHuifang Jang eZingxin Lin eBirun |
| author_sort | Li ePeiqian |
| collection | DOAJ |
| description | Velvet genes play critical roles in the regulation of diverse cellular processes. In current study, we identified the gene FocVel1, a homolog of Fusarium graminearum VelA, in the plant pathogenic fungus F. oxysporum f. sp. cucumerinum. This pathogen causes the destructive disease called cucumber Fusarium wilt, which severely affects the production and marketing of this vegetable worldwide. Transcript analyses revealed high expression of FocVel1 during conidiophore development. Disruption of the FocVel1 gene led to several phenotypic defects, including reduction in aerial hyphal formation and conidial production. The deletion mutant ⊿FocVel1 showed increased resistance to both osmotic stress and cell wall-damaging agents, but increased sensitivity to iprodione and prochloraz fungicides, which may be related to changes in cell wall components. In the process of biofilm formation in vitro, the mutant strain ⊿FocVel1 displayed not only a reduction in spore aggregation but also a delay in conidial germination on the polystyrene surface, which may result in defects in biofilm formation. Moreover, pathogenicity assays showed that the mutant ⊿FocVel1 exhibited impaired virulence in cucumber seedlings. And the genetic complementation of the mutant with the wild-type FocVel1 gene restored all the defects of the ⊿FocVel1. Taken together, the results of this study indicated that FocVel1 played a critical role in the regulation of various cellular processes and pathogenicity in F. oxysporum f. sp. cucumerinum. |
| first_indexed | 2024-12-13T21:06:39Z |
| format | Article |
| id | doaj.art-2e97a4f549c944ab8cdf4e0e2204ec14 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-13T21:06:39Z |
| publishDate | 2015-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-2e97a4f549c944ab8cdf4e0e2204ec142022-12-21T23:31:27ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2015-05-01610.3389/fpls.2015.00312118723FocVel1 influences asexual production, filamentous growth, biofilm formation, and virulence in Fusarium oxysporum f. sp. cucumerinumLi ePeiqian0Li ePeiqian1Li ePeiqian2Pu eXiaoming3Feng eBaozhen4Yang eQiyun5Shen eHuifang6Jang eZingxin7Lin eBirun8Key Laboratory of New Technique for Plant protection in Guangdong, Institute of Plant Protection, Guangdong Academy of Agricultural SciencesDepartment of Life Sciences, Yuncheng UniversityCollege of Agriculture Guangxi UniversityKey Laboratory of New Technique for Plant protection in Guangdong, Institute of Plant Protection, Guangdong Academy of Agricultural SciencesDepartment of Life Sciences, Yuncheng UniversityKey Laboratory of New Technique for Plant protection in Guangdong, Institute of Plant Protection, Guangdong Academy of Agricultural SciencesKey Laboratory of New Technique for Plant protection in Guangdong, Institute of Plant Protection, Guangdong Academy of Agricultural SciencesKey Laboratory of New Technique for Plant protection in Guangdong, Institute of Plant Protection, Guangdong Academy of Agricultural SciencesKey Laboratory of New Technique for Plant protection in Guangdong, Institute of Plant Protection, Guangdong Academy of Agricultural SciencesVelvet genes play critical roles in the regulation of diverse cellular processes. In current study, we identified the gene FocVel1, a homolog of Fusarium graminearum VelA, in the plant pathogenic fungus F. oxysporum f. sp. cucumerinum. This pathogen causes the destructive disease called cucumber Fusarium wilt, which severely affects the production and marketing of this vegetable worldwide. Transcript analyses revealed high expression of FocVel1 during conidiophore development. Disruption of the FocVel1 gene led to several phenotypic defects, including reduction in aerial hyphal formation and conidial production. The deletion mutant ⊿FocVel1 showed increased resistance to both osmotic stress and cell wall-damaging agents, but increased sensitivity to iprodione and prochloraz fungicides, which may be related to changes in cell wall components. In the process of biofilm formation in vitro, the mutant strain ⊿FocVel1 displayed not only a reduction in spore aggregation but also a delay in conidial germination on the polystyrene surface, which may result in defects in biofilm formation. Moreover, pathogenicity assays showed that the mutant ⊿FocVel1 exhibited impaired virulence in cucumber seedlings. And the genetic complementation of the mutant with the wild-type FocVel1 gene restored all the defects of the ⊿FocVel1. Taken together, the results of this study indicated that FocVel1 played a critical role in the regulation of various cellular processes and pathogenicity in F. oxysporum f. sp. cucumerinum.http://journal.frontiersin.org/Journal/10.3389/fpls.2015.00312/fullVirulenceBiofilmadherenceFusarium oxysporum f. sp. cucumerinumvelvet protein |
| spellingShingle | Li ePeiqian Li ePeiqian Li ePeiqian Pu eXiaoming Feng eBaozhen Yang eQiyun Shen eHuifang Jang eZingxin Lin eBirun FocVel1 influences asexual production, filamentous growth, biofilm formation, and virulence in Fusarium oxysporum f. sp. cucumerinum Frontiers in Plant Science Virulence Biofilm adherence Fusarium oxysporum f. sp. cucumerinum velvet protein |
| title | FocVel1 influences asexual production, filamentous growth, biofilm formation, and virulence in Fusarium oxysporum f. sp. cucumerinum |
| title_full | FocVel1 influences asexual production, filamentous growth, biofilm formation, and virulence in Fusarium oxysporum f. sp. cucumerinum |
| title_fullStr | FocVel1 influences asexual production, filamentous growth, biofilm formation, and virulence in Fusarium oxysporum f. sp. cucumerinum |
| title_full_unstemmed | FocVel1 influences asexual production, filamentous growth, biofilm formation, and virulence in Fusarium oxysporum f. sp. cucumerinum |
| title_short | FocVel1 influences asexual production, filamentous growth, biofilm formation, and virulence in Fusarium oxysporum f. sp. cucumerinum |
| title_sort | focvel1 influences asexual production filamentous growth biofilm formation and virulence in fusarium oxysporum f sp cucumerinum |
| topic | Virulence Biofilm adherence Fusarium oxysporum f. sp. cucumerinum velvet protein |
| url | http://journal.frontiersin.org/Journal/10.3389/fpls.2015.00312/full |
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