Whole-Genome Resequencing to Study Brucellosis Susceptibility in Sheep
Brucellosis is a zoonotic disease and a major public health problem. However, the genetic mechanism of brucellosis in sheep remains unclear. In this study, serum samples were collected from 6,358 sheep from the F2 population (Dorper sheep ♂ × Hu sheep ♀), and antibody levels were continuously measur...
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Frontiers Media S.A.
2021-07-01
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| Series: | Frontiers in Genetics |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fgene.2021.653927/full |
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| author | Xiaolong Li Qingmin Wu Xiaoxue Zhang Xiaoxue Zhang Chong Li Chong Li Deyin Zhang Guoze Li Yukun Zhang Yuan Zhao Zhaoguo Shi Weimin Wang Fadi Li Fadi Li Fadi Li |
| author_facet | Xiaolong Li Qingmin Wu Xiaoxue Zhang Xiaoxue Zhang Chong Li Chong Li Deyin Zhang Guoze Li Yukun Zhang Yuan Zhao Zhaoguo Shi Weimin Wang Fadi Li Fadi Li Fadi Li |
| author_sort | Xiaolong Li |
| collection | DOAJ |
| description | Brucellosis is a zoonotic disease and a major public health problem. However, the genetic mechanism of brucellosis in sheep remains unclear. In this study, serum samples were collected from 6,358 sheep from the F2 population (Dorper sheep ♂ × Hu sheep ♀), and antibody levels were continuously measured at 14 days and 1, 2, 3, 4, 5, 6, 7, 8, 9, and 10 months after administration of brucellosis vaccine. Finally, 19 brucellosis-resistant group (BRG) sheep and 22 brucellosis-susceptible group sheep (BSG) were screened for whole-genome sequencing. Using the fixation index, Fisher’s exact test, and chi-square test, a total of 205 candidate SNP sites were identified. Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis suggested that 138 candidate genes were significantly enriched in adherens junction (CTNNA3, PARD3, and PTPRM), cell adhesion molecules (NLGN1, CNTNAP2, NCAM1, and PTPRM), salivary secretion (LOC101102109, PRKG1, and ADCY2), and hippo signaling pathway (CTNNA3, YAP1, and PARD3). These findings provide valuable molecular markers for brucellosis resistance breeding in sheep and novel insights into the genetic mechanism of brucellosis resistance. |
| first_indexed | 2024-12-22T06:52:08Z |
| format | Article |
| id | doaj.art-2e97f60e2fc14c32b3a1148d0591e1ab |
| institution | Directory Open Access Journal |
| issn | 1664-8021 |
| language | English |
| last_indexed | 2024-12-22T06:52:08Z |
| publishDate | 2021-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Genetics |
| spelling | doaj.art-2e97f60e2fc14c32b3a1148d0591e1ab2022-12-21T18:35:06ZengFrontiers Media S.A.Frontiers in Genetics1664-80212021-07-011210.3389/fgene.2021.653927653927Whole-Genome Resequencing to Study Brucellosis Susceptibility in SheepXiaolong Li0Qingmin Wu1Xiaoxue Zhang2Xiaoxue Zhang3Chong Li4Chong Li5Deyin Zhang6Guoze Li7Yukun Zhang8Yuan Zhao9Zhaoguo Shi10Weimin Wang11Fadi Li12Fadi Li13Fadi Li14College of Animal Science and Technology, Gansu Agricultural University, Lanzhou, ChinaCollege of Veterinary Medicine, China Agricultural University, Beijing, ChinaCollege of Animal Science and Technology, Gansu Agricultural University, Lanzhou, ChinaEngineering Laboratory of Sheep Breeding and Reproduction Biotechnology in Gansu Province, Minqin, ChinaCollege of Animal Science and Technology, Gansu Agricultural University, Lanzhou, ChinaEngineering Laboratory of Sheep Breeding and Reproduction Biotechnology in Gansu Province, Minqin, ChinaCollege of Animal Science and Technology, Gansu Agricultural University, Lanzhou, ChinaCollege of Animal Science and Technology, Gansu Agricultural University, Lanzhou, ChinaCollege of Animal Science and Technology, Gansu Agricultural University, Lanzhou, ChinaCollege of Animal Science and Technology, Gansu Agricultural University, Lanzhou, ChinaCollege of Animal Science and Technology, Gansu Agricultural University, Lanzhou, ChinaCollege of Animal Science and Technology, Gansu Agricultural University, Lanzhou, ChinaCollege of Animal Science and Technology, Gansu Agricultural University, Lanzhou, ChinaEngineering Laboratory of Sheep Breeding and Reproduction Biotechnology in Gansu Province, Minqin, ChinaThe State Key Laboratory of Grassland Agro-ecosystems, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou, ChinaBrucellosis is a zoonotic disease and a major public health problem. However, the genetic mechanism of brucellosis in sheep remains unclear. In this study, serum samples were collected from 6,358 sheep from the F2 population (Dorper sheep ♂ × Hu sheep ♀), and antibody levels were continuously measured at 14 days and 1, 2, 3, 4, 5, 6, 7, 8, 9, and 10 months after administration of brucellosis vaccine. Finally, 19 brucellosis-resistant group (BRG) sheep and 22 brucellosis-susceptible group sheep (BSG) were screened for whole-genome sequencing. Using the fixation index, Fisher’s exact test, and chi-square test, a total of 205 candidate SNP sites were identified. Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis suggested that 138 candidate genes were significantly enriched in adherens junction (CTNNA3, PARD3, and PTPRM), cell adhesion molecules (NLGN1, CNTNAP2, NCAM1, and PTPRM), salivary secretion (LOC101102109, PRKG1, and ADCY2), and hippo signaling pathway (CTNNA3, YAP1, and PARD3). These findings provide valuable molecular markers for brucellosis resistance breeding in sheep and novel insights into the genetic mechanism of brucellosis resistance.https://www.frontiersin.org/articles/10.3389/fgene.2021.653927/fullbrucellosiswhole-genome resequencingFSTFisher’s exact testchi-square testsheep |
| spellingShingle | Xiaolong Li Qingmin Wu Xiaoxue Zhang Xiaoxue Zhang Chong Li Chong Li Deyin Zhang Guoze Li Yukun Zhang Yuan Zhao Zhaoguo Shi Weimin Wang Fadi Li Fadi Li Fadi Li Whole-Genome Resequencing to Study Brucellosis Susceptibility in Sheep Frontiers in Genetics brucellosis whole-genome resequencing FST Fisher’s exact test chi-square test sheep |
| title | Whole-Genome Resequencing to Study Brucellosis Susceptibility in Sheep |
| title_full | Whole-Genome Resequencing to Study Brucellosis Susceptibility in Sheep |
| title_fullStr | Whole-Genome Resequencing to Study Brucellosis Susceptibility in Sheep |
| title_full_unstemmed | Whole-Genome Resequencing to Study Brucellosis Susceptibility in Sheep |
| title_short | Whole-Genome Resequencing to Study Brucellosis Susceptibility in Sheep |
| title_sort | whole genome resequencing to study brucellosis susceptibility in sheep |
| topic | brucellosis whole-genome resequencing FST Fisher’s exact test chi-square test sheep |
| url | https://www.frontiersin.org/articles/10.3389/fgene.2021.653927/full |
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