Investigation of Various Tissue Culture Monolayers Sensitivity in Detection of Clostridium difficile Toxin
Backround: Clostridium difficile is the most common cause of nosocomial diarrhea. It is usually a consequence of antibiotic treatment, but sporadic cases can occur. The purpose of this study was to investigate five tissue culture monolayers sensitivity in detection of C. difficile-toxin. Methods...
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2008-03-01
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| Series: | Iranian Journal of Public Health |
| Subjects: | |
| Online Access: | https://ijph.tums.ac.ir/index.php/ijph/article/view/2078 |
| _version_ | 1818884096651165696 |
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| author | MH Salari N Badami N Sadeghifard F Amin Harati |
| author_facet | MH Salari N Badami N Sadeghifard F Amin Harati |
| author_sort | MH Salari |
| collection | DOAJ |
| description | Backround: Clostridium difficile is the most common cause of nosocomial diarrhea. It is usually a consequence of antibiotic treatment, but sporadic cases can occur. The purpose of this study was to investigate five tissue culture monolayers sensitivity in detection of C. difficile-toxin.
Methods: A total of 402 stool samples from patients with nosocomial diarrhea hospitalized in three hospitals of Tehran University of Medical Sciences (TUMS) were collected. The samples were cultured on a selective cycloserine cefoxitin fructose agar (CCFA) and incubated in anaerobic conditions, at 37 °C for 4 days. Isolates were characterized to species level by conventional biochemical tests. Bacterial cytotoxicity was assayed on five tissue culture monolayers.
Results: Our findings show that of the total patients, 24 toxigenic C. difficile (6%) were isolated. All 24 C. difficile toxins showed cytotoxic effect at ³ 1:10 dilution on Hela, Hep2, Vero, McCoy and Mdck cells after 16, 20, 24, 24 and 30 hours, respectively. C. difficile toxin showed cytotoxic effect at ³ 1:100 dilutions only on Hela cell monolayer after 48 hours.
Conclusion: Hela cell monolayer may be a satisfactory substitute for the detection of C. difficile toxin in clinical specimens. |
| first_indexed | 2024-12-19T15:44:07Z |
| format | Article |
| id | doaj.art-2e98140aa4c54eb1931e035d1aae0bd0 |
| institution | Directory Open Access Journal |
| issn | 2251-6085 2251-6093 |
| language | English |
| last_indexed | 2024-12-19T15:44:07Z |
| publishDate | 2008-03-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Public Health |
| spelling | doaj.art-2e98140aa4c54eb1931e035d1aae0bd02022-12-21T20:15:24ZengTehran University of Medical SciencesIranian Journal of Public Health2251-60852251-60932008-03-01371Investigation of Various Tissue Culture Monolayers Sensitivity in Detection of Clostridium difficile Toxin MH Salari0 N Badami1 N Sadeghifard2 F Amin Harati3 Backround: Clostridium difficile is the most common cause of nosocomial diarrhea. It is usually a consequence of antibiotic treatment, but sporadic cases can occur. The purpose of this study was to investigate five tissue culture monolayers sensitivity in detection of C. difficile-toxin. Methods: A total of 402 stool samples from patients with nosocomial diarrhea hospitalized in three hospitals of Tehran University of Medical Sciences (TUMS) were collected. The samples were cultured on a selective cycloserine cefoxitin fructose agar (CCFA) and incubated in anaerobic conditions, at 37 °C for 4 days. Isolates were characterized to species level by conventional biochemical tests. Bacterial cytotoxicity was assayed on five tissue culture monolayers. Results: Our findings show that of the total patients, 24 toxigenic C. difficile (6%) were isolated. All 24 C. difficile toxins showed cytotoxic effect at ³ 1:10 dilution on Hela, Hep2, Vero, McCoy and Mdck cells after 16, 20, 24, 24 and 30 hours, respectively. C. difficile toxin showed cytotoxic effect at ³ 1:100 dilutions only on Hela cell monolayer after 48 hours. Conclusion: Hela cell monolayer may be a satisfactory substitute for the detection of C. difficile toxin in clinical specimens.https://ijph.tums.ac.ir/index.php/ijph/article/view/2078CPeVarious tissue culture |
| spellingShingle | MH Salari N Badami N Sadeghifard F Amin Harati Investigation of Various Tissue Culture Monolayers Sensitivity in Detection of Clostridium difficile Toxin Iranian Journal of Public Health CPe Various tissue culture |
| title | Investigation of Various Tissue Culture Monolayers Sensitivity in Detection of Clostridium difficile Toxin |
| title_full | Investigation of Various Tissue Culture Monolayers Sensitivity in Detection of Clostridium difficile Toxin |
| title_fullStr | Investigation of Various Tissue Culture Monolayers Sensitivity in Detection of Clostridium difficile Toxin |
| title_full_unstemmed | Investigation of Various Tissue Culture Monolayers Sensitivity in Detection of Clostridium difficile Toxin |
| title_short | Investigation of Various Tissue Culture Monolayers Sensitivity in Detection of Clostridium difficile Toxin |
| title_sort | investigation of various tissue culture monolayers sensitivity in detection of clostridium difficile toxin |
| topic | CPe Various tissue culture |
| url | https://ijph.tums.ac.ir/index.php/ijph/article/view/2078 |
| work_keys_str_mv | AT mhsalari investigationofvarioustissueculturemonolayerssensitivityindetectionofclostridiumdifficiletoxin AT nbadami investigationofvarioustissueculturemonolayerssensitivityindetectionofclostridiumdifficiletoxin AT nsadeghifard investigationofvarioustissueculturemonolayerssensitivityindetectionofclostridiumdifficiletoxin AT faminharati investigationofvarioustissueculturemonolayerssensitivityindetectionofclostridiumdifficiletoxin |