Stromal-epithelial interaction induces GALNT14 in prostate carcinoma cells
IntroductionCell-cell communication is an important process in healthy tissue but also gains enhanced attention regarding pathological tissue. To date, the tumor microenvironment is gradually brought into focus when studying tumorigenesis. In the prostate gland, stromal and epithelial cells greatly...
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| Format: | Article |
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Frontiers Media S.A.
2023-08-01
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| Series: | Frontiers in Oncology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fonc.2023.1212585/full |
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| author | Elena D. Czyrnik Marc Wiesehöfer Jaroslaw T. Dankert Sven Wach Mathias Wagner Martin Spahn Martin Spahn Marianna Kruithof de Julio Marianna Kruithof de Julio Gunther Wennemuth |
| author_facet | Elena D. Czyrnik Marc Wiesehöfer Jaroslaw T. Dankert Sven Wach Mathias Wagner Martin Spahn Martin Spahn Marianna Kruithof de Julio Marianna Kruithof de Julio Gunther Wennemuth |
| author_sort | Elena D. Czyrnik |
| collection | DOAJ |
| description | IntroductionCell-cell communication is an important process in healthy tissue but also gains enhanced attention regarding pathological tissue. To date, the tumor microenvironment is gradually brought into focus when studying tumorigenesis. In the prostate gland, stromal and epithelial cells greatly interact to maintain homeostasis or tissue integrity. This study focuses on an indirect communication via soluble factors.MethodsTo investigate the cell-cell interaction via soluble factors, the prostate carcinoma cell line LNCaP and the stromal primary cells p21 were co-cultured without direct contact and RNA was isolated at defined time points. Differences in gene expression were finally analyzed by RNA sequencing.ResultsRNA sequencing revealed a time-depending differential expression profile. Selected factors were subsequently characterized at molecular level and analyzed in human prostate tissue of different developmental stages as well as pathology. GALNT14 was one of the highest induced co-culture-specific genes in LNCaP cells. Detection in healthy tissue and BPH revealed an age-dependent decrease in GALNT14 expression. Moreover, in prostate carcinoma, GALNT14 expression heavily varied independent of the Gleason score.ConclusionOverall, this work provides a basis for further studies related to paracrine stromal-epithelial interaction in prostate carcinoma and highlights the importance of GALNT14. |
| first_indexed | 2024-03-12T14:04:32Z |
| format | Article |
| id | doaj.art-2e9955f506ff4e5da1eeed68d036f8f2 |
| institution | Directory Open Access Journal |
| issn | 2234-943X |
| language | English |
| last_indexed | 2024-03-12T14:04:32Z |
| publishDate | 2023-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Oncology |
| spelling | doaj.art-2e9955f506ff4e5da1eeed68d036f8f22023-08-21T15:39:18ZengFrontiers Media S.A.Frontiers in Oncology2234-943X2023-08-011310.3389/fonc.2023.12125851212585Stromal-epithelial interaction induces GALNT14 in prostate carcinoma cellsElena D. Czyrnik0Marc Wiesehöfer1Jaroslaw T. Dankert2Sven Wach3Mathias Wagner4Martin Spahn5Martin Spahn6Marianna Kruithof de Julio7Marianna Kruithof de Julio8Gunther Wennemuth9University Hospital Essen, Department of Anatomy, Essen, GermanyUniversity Hospital Essen, Department of Anatomy, Essen, GermanyUniversity Hospital Essen, Department of Anatomy, Essen, GermanyDepartment of Urology and Pediatric Urology, University Hospital Erlangen, Erlangen, GermanyUniversity Hospital Saarland, Department of General and Special Pathology, Homburg, GermanyLindenhofspital Bern, Department of Urology, Bern, SwitzerlandUniversity Hospital Essen, Department of Urology, Essen, GermanyDepartment for BioMedical Research, Urology Research Laboratory, University of Bern, Bern, SwitzerlandDepartment of Urology, Inselspital, Bern University Hospital, Bern, SwitzerlandUniversity Hospital Essen, Department of Anatomy, Essen, GermanyIntroductionCell-cell communication is an important process in healthy tissue but also gains enhanced attention regarding pathological tissue. To date, the tumor microenvironment is gradually brought into focus when studying tumorigenesis. In the prostate gland, stromal and epithelial cells greatly interact to maintain homeostasis or tissue integrity. This study focuses on an indirect communication via soluble factors.MethodsTo investigate the cell-cell interaction via soluble factors, the prostate carcinoma cell line LNCaP and the stromal primary cells p21 were co-cultured without direct contact and RNA was isolated at defined time points. Differences in gene expression were finally analyzed by RNA sequencing.ResultsRNA sequencing revealed a time-depending differential expression profile. Selected factors were subsequently characterized at molecular level and analyzed in human prostate tissue of different developmental stages as well as pathology. GALNT14 was one of the highest induced co-culture-specific genes in LNCaP cells. Detection in healthy tissue and BPH revealed an age-dependent decrease in GALNT14 expression. Moreover, in prostate carcinoma, GALNT14 expression heavily varied independent of the Gleason score.ConclusionOverall, this work provides a basis for further studies related to paracrine stromal-epithelial interaction in prostate carcinoma and highlights the importance of GALNT14.https://www.frontiersin.org/articles/10.3389/fonc.2023.1212585/fullprostate tissueprostate carcinomastromal-epithelial interactionparacrine interactionco-cultureGALNT14 |
| spellingShingle | Elena D. Czyrnik Marc Wiesehöfer Jaroslaw T. Dankert Sven Wach Mathias Wagner Martin Spahn Martin Spahn Marianna Kruithof de Julio Marianna Kruithof de Julio Gunther Wennemuth Stromal-epithelial interaction induces GALNT14 in prostate carcinoma cells Frontiers in Oncology prostate tissue prostate carcinoma stromal-epithelial interaction paracrine interaction co-culture GALNT14 |
| title | Stromal-epithelial interaction induces GALNT14 in prostate carcinoma cells |
| title_full | Stromal-epithelial interaction induces GALNT14 in prostate carcinoma cells |
| title_fullStr | Stromal-epithelial interaction induces GALNT14 in prostate carcinoma cells |
| title_full_unstemmed | Stromal-epithelial interaction induces GALNT14 in prostate carcinoma cells |
| title_short | Stromal-epithelial interaction induces GALNT14 in prostate carcinoma cells |
| title_sort | stromal epithelial interaction induces galnt14 in prostate carcinoma cells |
| topic | prostate tissue prostate carcinoma stromal-epithelial interaction paracrine interaction co-culture GALNT14 |
| url | https://www.frontiersin.org/articles/10.3389/fonc.2023.1212585/full |
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