First two fungemia cases caused by Candida haemulonii var. vulnera in China with emerged antifungal resistance
Candida haemulonii var. vulnera is a rare variant of C. haemulonii, which has been previously reported to cause human infections. Owing to the close kinship between C. haemulonii sensu stricto and C. haemulonii var. vulnera, accurate identification of C. haemulonii var. vulnera relied on DNA sequenc...
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| Format: | Article |
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Frontiers Media S.A.
2022-11-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.1036351/full |
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| author | Xin-Fei Chen Xin-Fei Chen Xin-Fei Chen Xin Hou Han Zhang Han Zhang Xin-Miao Jia Xin-Miao Jia Li-Ping Ning Wei Cao Xin Fan Jing-Jing Huang Jing-Jing Huang Jing-Jing Huang Wen-Hang Yang Wen-Hang Yang Ge Zhang Ge Zhang Jing-Jia Zhang Jing-Jia Zhang Wei Kang Wei Kang Meng Xiao Meng Xiao Ying-Chun Xu Ying-Chun Xu |
| author_facet | Xin-Fei Chen Xin-Fei Chen Xin-Fei Chen Xin Hou Han Zhang Han Zhang Xin-Miao Jia Xin-Miao Jia Li-Ping Ning Wei Cao Xin Fan Jing-Jing Huang Jing-Jing Huang Jing-Jing Huang Wen-Hang Yang Wen-Hang Yang Ge Zhang Ge Zhang Jing-Jia Zhang Jing-Jia Zhang Wei Kang Wei Kang Meng Xiao Meng Xiao Ying-Chun Xu Ying-Chun Xu |
| author_sort | Xin-Fei Chen |
| collection | DOAJ |
| description | Candida haemulonii var. vulnera is a rare variant of C. haemulonii, which has been previously reported to cause human infections. Owing to the close kinship between C. haemulonii sensu stricto and C. haemulonii var. vulnera, accurate identification of C. haemulonii var. vulnera relied on DNA sequencing assay targeting, for example, rDNA internal transcribed spacer (ITS) region. In this work, two strains of C. haemulonii var. vulnera were collected from the China Hospital Invasive Fungal Surveillance Net (CHIF-NET). The identification capacity of three matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and VITEK 2 YST ID biochemical methods were evaluated against ITS sequencing. In addition, antifungal susceptibility testing was performed using Sensititre YeastOne. Moreover, we comprehensively screened drug-resistant related genes by whole-genome sequencing. The two strains were not correctly identified to species variant level using MALDI-TOF MS and YST ID cards. Both strains were resistant to amphotericin B (minimum inhibitory concentration [MIC] > 2 μg/ml). Moreover, strain F4564 and F4584 exhibited high MIC to fluconazole (>256 μg/ml) and 5-flucytosine (>64 μg/ml), respectively, which were supposed to result from key amino acid substitutions Y132F and G307A in Erg11p and V58fs and G60K substitutions in Fur1p. The rare species C. haemulonii var. vulnera has emerged in China, and such drug-resistant fungal species that can cause invasive diseases require further close attention. |
| first_indexed | 2024-04-11T08:07:52Z |
| format | Article |
| id | doaj.art-2e9afaec2deb413ea7fbcceb504a2c32 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-11T08:07:52Z |
| publishDate | 2022-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-2e9afaec2deb413ea7fbcceb504a2c322022-12-22T04:35:27ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2022-11-011310.3389/fmicb.2022.10363511036351First two fungemia cases caused by Candida haemulonii var. vulnera in China with emerged antifungal resistanceXin-Fei Chen0Xin-Fei Chen1Xin-Fei Chen2Xin Hou3Han Zhang4Han Zhang5Xin-Miao Jia6Xin-Miao Jia7Li-Ping Ning8Wei Cao9Xin Fan10Jing-Jing Huang11Jing-Jing Huang12Jing-Jing Huang13Wen-Hang Yang14Wen-Hang Yang15Ge Zhang16Ge Zhang17Jing-Jia Zhang18Jing-Jia Zhang19Wei Kang20Wei Kang21Meng Xiao22Meng Xiao23Ying-Chun Xu24Ying-Chun Xu25Department of Laboratory Medicine, State Key Laboratory of Complex Severe and Rare Diseases, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, ChinaGraduate School, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, ChinaBeijing Key Laboratory for Mechanisms Research and Precision Diagnosis of Invasive Fungal Diseases (BZ0447), Beijing, ChinaDepartment of Laboratory Medicine, Peking University Third Hospital, Beijing, ChinaDepartment of Laboratory Medicine, State Key Laboratory of Complex Severe and Rare Diseases, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, ChinaBeijing Key Laboratory for Mechanisms Research and Precision Diagnosis of Invasive Fungal Diseases (BZ0447), Beijing, ChinaBeijing Key Laboratory for Mechanisms Research and Precision Diagnosis of Invasive Fungal Diseases (BZ0447), Beijing, ChinaMedical Research Center, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, ChinaDepartment of Laboratory Medicine, No.908 Hospital of Joint Logistics Support Force, Nanchang, Jiangxi, ChinaDepartment of Laboratory Medicine, The Second Xiangya Hospital, Central South University, Changsha, Hubei, ChinaDepartment of Infectious Diseases and Clinical Microbiology, Beijing Institute of Respiratory Medicine, Beijing Chao-Yang Hospital, Capital Medical University, Beijing, ChinaDepartment of Laboratory Medicine, State Key Laboratory of Complex Severe and Rare Diseases, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, ChinaGraduate School, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, ChinaBeijing Key Laboratory for Mechanisms Research and Precision Diagnosis of Invasive Fungal Diseases (BZ0447), Beijing, ChinaDepartment of Laboratory Medicine, State Key Laboratory of Complex Severe and Rare Diseases, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, ChinaBeijing Key Laboratory for Mechanisms Research and Precision Diagnosis of Invasive Fungal Diseases (BZ0447), Beijing, ChinaDepartment of Laboratory Medicine, State Key Laboratory of Complex Severe and Rare Diseases, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, ChinaBeijing Key Laboratory for Mechanisms Research and Precision Diagnosis of Invasive Fungal Diseases (BZ0447), Beijing, ChinaDepartment of Laboratory Medicine, State Key Laboratory of Complex Severe and Rare Diseases, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, ChinaBeijing Key Laboratory for Mechanisms Research and Precision Diagnosis of Invasive Fungal Diseases (BZ0447), Beijing, ChinaDepartment of Laboratory Medicine, State Key Laboratory of Complex Severe and Rare Diseases, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, ChinaBeijing Key Laboratory for Mechanisms Research and Precision Diagnosis of Invasive Fungal Diseases (BZ0447), Beijing, ChinaDepartment of Laboratory Medicine, State Key Laboratory of Complex Severe and Rare Diseases, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, ChinaBeijing Key Laboratory for Mechanisms Research and Precision Diagnosis of Invasive Fungal Diseases (BZ0447), Beijing, ChinaDepartment of Laboratory Medicine, State Key Laboratory of Complex Severe and Rare Diseases, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, ChinaBeijing Key Laboratory for Mechanisms Research and Precision Diagnosis of Invasive Fungal Diseases (BZ0447), Beijing, ChinaCandida haemulonii var. vulnera is a rare variant of C. haemulonii, which has been previously reported to cause human infections. Owing to the close kinship between C. haemulonii sensu stricto and C. haemulonii var. vulnera, accurate identification of C. haemulonii var. vulnera relied on DNA sequencing assay targeting, for example, rDNA internal transcribed spacer (ITS) region. In this work, two strains of C. haemulonii var. vulnera were collected from the China Hospital Invasive Fungal Surveillance Net (CHIF-NET). The identification capacity of three matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and VITEK 2 YST ID biochemical methods were evaluated against ITS sequencing. In addition, antifungal susceptibility testing was performed using Sensititre YeastOne. Moreover, we comprehensively screened drug-resistant related genes by whole-genome sequencing. The two strains were not correctly identified to species variant level using MALDI-TOF MS and YST ID cards. Both strains were resistant to amphotericin B (minimum inhibitory concentration [MIC] > 2 μg/ml). Moreover, strain F4564 and F4584 exhibited high MIC to fluconazole (>256 μg/ml) and 5-flucytosine (>64 μg/ml), respectively, which were supposed to result from key amino acid substitutions Y132F and G307A in Erg11p and V58fs and G60K substitutions in Fur1p. The rare species C. haemulonii var. vulnera has emerged in China, and such drug-resistant fungal species that can cause invasive diseases require further close attention.https://www.frontiersin.org/articles/10.3389/fmicb.2022.1036351/fullCandida haemulonii var. vulneraantifungal susceptibilityERG11FUR1whole-genome sequencedrug resistant mechanisms |
| spellingShingle | Xin-Fei Chen Xin-Fei Chen Xin-Fei Chen Xin Hou Han Zhang Han Zhang Xin-Miao Jia Xin-Miao Jia Li-Ping Ning Wei Cao Xin Fan Jing-Jing Huang Jing-Jing Huang Jing-Jing Huang Wen-Hang Yang Wen-Hang Yang Ge Zhang Ge Zhang Jing-Jia Zhang Jing-Jia Zhang Wei Kang Wei Kang Meng Xiao Meng Xiao Ying-Chun Xu Ying-Chun Xu First two fungemia cases caused by Candida haemulonii var. vulnera in China with emerged antifungal resistance Frontiers in Microbiology Candida haemulonii var. vulnera antifungal susceptibility ERG11 FUR1 whole-genome sequence drug resistant mechanisms |
| title | First two fungemia cases caused by Candida haemulonii var. vulnera in China with emerged antifungal resistance |
| title_full | First two fungemia cases caused by Candida haemulonii var. vulnera in China with emerged antifungal resistance |
| title_fullStr | First two fungemia cases caused by Candida haemulonii var. vulnera in China with emerged antifungal resistance |
| title_full_unstemmed | First two fungemia cases caused by Candida haemulonii var. vulnera in China with emerged antifungal resistance |
| title_short | First two fungemia cases caused by Candida haemulonii var. vulnera in China with emerged antifungal resistance |
| title_sort | first two fungemia cases caused by candida haemulonii var vulnera in china with emerged antifungal resistance |
| topic | Candida haemulonii var. vulnera antifungal susceptibility ERG11 FUR1 whole-genome sequence drug resistant mechanisms |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.1036351/full |
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