| Summary: | Background: Abandoned mining sites are becoming increasingly common due to anthropogenic activities. Consequently, external-soil spray seeding technology has attracted increasing attention as a strategy to remediate them. However, significant challenges remain that greatly inhibit the efficacy of such technologies, such as insufficient nutrients available for plants. Methods: For this study, we designed an experiment, which involved the addition of mineral-solubilizing microorganisms and <i>R. pseudoacacia</i> seedlings to the external-soil spray seeding (ESSS) substrate, and measured the soil nutrients, enzyme activities, and root growth of <i>R. pseudoacacia</i>. Results: First, the combination of certain mineral-solubilizing microorganisms with ESSS advanced its efficiency by increasing the availability of soil nutrients and soil enzymatic activities in association with <i>R. pseudoacacia</i>. Furthermore, the improvement of root growth of <i>R. pseudoacacia</i> was intimately related to soil nutrients, particularly for soil total nitrogen (TN) and total sulfur (TS). In general, the effects of the J2 (combined <i>Bacillus thuringiensis</i> and <i>Gongronella butleri</i>) treatment for soil nutrients, enzyme activities, and plant growth were the strongest. Conclusion: In summary, the results of our experiment revealed that these mineral-solubilizing microorganisms conveyed a promotional effect on <i>R. pseudoacacia</i> seedlings by increasing the soil nutrient content. These results provide basic data and microbial resources for the development and applications of mineral-solubilizing microorganisms for abandoned mine remediation.
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