Redesign and start up operation a bioreactor at pilot plant scale for solid-state fermentation processes

A stainless steel bioreactor was redesigned in order to facilite the production of microbial protein by solid-state fermentation. A double-helix stirrer was built to replace a blade-stirrer. Mixing time was set at 12 min every two hours. An air-manifold was built and installed at the bottom of the...

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Main Authors: Nicolino Bracho, Miguel Rondón, Cateryna Aiello, Alexis Ferrer, Arelis López
Format: Article
Language:English
Published: Universidad del Zulia 2010-11-01
Series:Revista Técnica de la Facultad de Ingeniería
Subjects:
Online Access:https://www.produccioncientificaluz.org/index.php/tecnica/article/view/5525
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author Nicolino Bracho
Miguel Rondón
Cateryna Aiello
Alexis Ferrer
Arelis López
author_facet Nicolino Bracho
Miguel Rondón
Cateryna Aiello
Alexis Ferrer
Arelis López
author_sort Nicolino Bracho
collection DOAJ
description A stainless steel bioreactor was redesigned in order to facilite the production of microbial protein by solid-state fermentation. A double-helix stirrer was built to replace a blade-stirrer. Mixing time was set at 12 min every two hours. An air-manifold was built and installed at the bottom of the reactor. It allowed uniform bubbling in the water reservoir, providing a continuous flow of saturated air through the biomass. The intermittent mixing provided it a uniform substrate with minimun damage to the mycelial biomass. Along with the saturated air, allowed removal of the metabolic heat produced and prevented formation of dead zones in the biomass. A fermentation with Trichoderma reesei QM 9414 on water-treated sugar cane bagasse was carried out to test the operation of the bioreactor. The fermented product reached 7% crude protein at 48 h. A maximum of 10.96% was reached at 96h. This value was much higher than the laboratory value, confirming that the design allows a more efficient fermentation process.
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spelling doaj.art-2e9dff9b41204ab09ee76798002cec1b2022-12-22T04:18:59ZengUniversidad del ZuliaRevista Técnica de la Facultad de Ingeniería0254-07702477-93772010-11-01201Redesign and start up operation a bioreactor at pilot plant scale for solid-state fermentation processesNicolino Bracho0Miguel Rondón1Cateryna Aiello2Alexis Ferrer3Arelis López4Universidad del Zulia-VenezuelaUniversidad del Zulia-VenezuelaUniversidad del Zulia-VenezuelaUniversidad del Zulia-VenezuelaUniversidad del Zulia-Venezuela A stainless steel bioreactor was redesigned in order to facilite the production of microbial protein by solid-state fermentation. A double-helix stirrer was built to replace a blade-stirrer. Mixing time was set at 12 min every two hours. An air-manifold was built and installed at the bottom of the reactor. It allowed uniform bubbling in the water reservoir, providing a continuous flow of saturated air through the biomass. The intermittent mixing provided it a uniform substrate with minimun damage to the mycelial biomass. Along with the saturated air, allowed removal of the metabolic heat produced and prevented formation of dead zones in the biomass. A fermentation with Trichoderma reesei QM 9414 on water-treated sugar cane bagasse was carried out to test the operation of the bioreactor. The fermented product reached 7% crude protein at 48 h. A maximum of 10.96% was reached at 96h. This value was much higher than the laboratory value, confirming that the design allows a more efficient fermentation process. https://www.produccioncientificaluz.org/index.php/tecnica/article/view/5525bioreactorsolid state fermentationssugar cane bagassemicrobial proteinTrichoderma reesei
spellingShingle Nicolino Bracho
Miguel Rondón
Cateryna Aiello
Alexis Ferrer
Arelis López
Redesign and start up operation a bioreactor at pilot plant scale for solid-state fermentation processes
Revista Técnica de la Facultad de Ingeniería
bioreactor
solid state fermentations
sugar cane bagasse
microbial protein
Trichoderma reesei
title Redesign and start up operation a bioreactor at pilot plant scale for solid-state fermentation processes
title_full Redesign and start up operation a bioreactor at pilot plant scale for solid-state fermentation processes
title_fullStr Redesign and start up operation a bioreactor at pilot plant scale for solid-state fermentation processes
title_full_unstemmed Redesign and start up operation a bioreactor at pilot plant scale for solid-state fermentation processes
title_short Redesign and start up operation a bioreactor at pilot plant scale for solid-state fermentation processes
title_sort redesign and start up operation a bioreactor at pilot plant scale for solid state fermentation processes
topic bioreactor
solid state fermentations
sugar cane bagasse
microbial protein
Trichoderma reesei
url https://www.produccioncientificaluz.org/index.php/tecnica/article/view/5525
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AT miguelrondon redesignandstartupoperationabioreactoratpilotplantscaleforsolidstatefermentationprocesses
AT caterynaaiello redesignandstartupoperationabioreactoratpilotplantscaleforsolidstatefermentationprocesses
AT alexisferrer redesignandstartupoperationabioreactoratpilotplantscaleforsolidstatefermentationprocesses
AT arelislopez redesignandstartupoperationabioreactoratpilotplantscaleforsolidstatefermentationprocesses