Towards Improved Molecular Identification Tools in Fine Fescue (Festuca L., Poaceae) Turfgrasses: Nuclear Genome Size, Ploidy, and Chloroplast Genome Sequencing
Fine fescues (Festuca L., Poaceae) are turfgrass species that perform well in low-input environments. Based on morphological characteristics, the most commonly-utilized fine fescues are divided into five taxa: three are subspecies within F. rubra L. and the remaining two are treated as species withi...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2019-12-01
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| Series: | Frontiers in Genetics |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fgene.2019.01223/full |
| _version_ | 1819054068891385856 |
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| author | Yinjie Qiu Cory D. Hirsch Ya Yang Eric Watkins |
| author_facet | Yinjie Qiu Cory D. Hirsch Ya Yang Eric Watkins |
| author_sort | Yinjie Qiu |
| collection | DOAJ |
| description | Fine fescues (Festuca L., Poaceae) are turfgrass species that perform well in low-input environments. Based on morphological characteristics, the most commonly-utilized fine fescues are divided into five taxa: three are subspecies within F. rubra L. and the remaining two are treated as species within the F. ovina L. complex. Morphologically, these five taxa are very similar; both identification and classification of fine fescues remain challenging. In an effort to develop identification methods for fescues, we used flow cytometry to estimate genome size and ploidy level and sequenced the chloroplast genome of all five taxa. Fine fescue chloroplast genome sizes ranged from 133,331 to 133,841 bp and contained 113–114 genes. Phylogenetic relationship reconstruction using whole chloroplast genome sequences agreed with previous work based on morphology. Comparative genomics suggested unique repeat signatures for each fine fescue taxon that could potentially be used for marker development for taxon identification. |
| first_indexed | 2024-12-21T12:45:45Z |
| format | Article |
| id | doaj.art-2e9eca76e95c48b092b495c47d61ca24 |
| institution | Directory Open Access Journal |
| issn | 1664-8021 |
| language | English |
| last_indexed | 2024-12-21T12:45:45Z |
| publishDate | 2019-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Genetics |
| spelling | doaj.art-2e9eca76e95c48b092b495c47d61ca242022-12-21T19:03:38ZengFrontiers Media S.A.Frontiers in Genetics1664-80212019-12-011010.3389/fgene.2019.01223491796Towards Improved Molecular Identification Tools in Fine Fescue (Festuca L., Poaceae) Turfgrasses: Nuclear Genome Size, Ploidy, and Chloroplast Genome SequencingYinjie Qiu0Cory D. Hirsch1Ya Yang2Eric Watkins3Department of Horticultural Science, University of Minnesota, St. Paul, MN, United StatesDepartment of Plant Pathology, University of Minnesota, St. Paul, MN, United StatesDepartment of Plant and Microbial Biology, University of Minnesota, St. Paul, MN, United StatesDepartment of Horticultural Science, University of Minnesota, St. Paul, MN, United StatesFine fescues (Festuca L., Poaceae) are turfgrass species that perform well in low-input environments. Based on morphological characteristics, the most commonly-utilized fine fescues are divided into five taxa: three are subspecies within F. rubra L. and the remaining two are treated as species within the F. ovina L. complex. Morphologically, these five taxa are very similar; both identification and classification of fine fescues remain challenging. In an effort to develop identification methods for fescues, we used flow cytometry to estimate genome size and ploidy level and sequenced the chloroplast genome of all five taxa. Fine fescue chloroplast genome sizes ranged from 133,331 to 133,841 bp and contained 113–114 genes. Phylogenetic relationship reconstruction using whole chloroplast genome sequences agreed with previous work based on morphology. Comparative genomics suggested unique repeat signatures for each fine fescue taxon that could potentially be used for marker development for taxon identification.https://www.frontiersin.org/article/10.3389/fgene.2019.01223/fullfine fescuechloroplast genomephylogenycomparative genomicslow input turfgrass |
| spellingShingle | Yinjie Qiu Cory D. Hirsch Ya Yang Eric Watkins Towards Improved Molecular Identification Tools in Fine Fescue (Festuca L., Poaceae) Turfgrasses: Nuclear Genome Size, Ploidy, and Chloroplast Genome Sequencing Frontiers in Genetics fine fescue chloroplast genome phylogeny comparative genomics low input turfgrass |
| title | Towards Improved Molecular Identification Tools in Fine Fescue (Festuca L., Poaceae) Turfgrasses: Nuclear Genome Size, Ploidy, and Chloroplast Genome Sequencing |
| title_full | Towards Improved Molecular Identification Tools in Fine Fescue (Festuca L., Poaceae) Turfgrasses: Nuclear Genome Size, Ploidy, and Chloroplast Genome Sequencing |
| title_fullStr | Towards Improved Molecular Identification Tools in Fine Fescue (Festuca L., Poaceae) Turfgrasses: Nuclear Genome Size, Ploidy, and Chloroplast Genome Sequencing |
| title_full_unstemmed | Towards Improved Molecular Identification Tools in Fine Fescue (Festuca L., Poaceae) Turfgrasses: Nuclear Genome Size, Ploidy, and Chloroplast Genome Sequencing |
| title_short | Towards Improved Molecular Identification Tools in Fine Fescue (Festuca L., Poaceae) Turfgrasses: Nuclear Genome Size, Ploidy, and Chloroplast Genome Sequencing |
| title_sort | towards improved molecular identification tools in fine fescue festuca l poaceae turfgrasses nuclear genome size ploidy and chloroplast genome sequencing |
| topic | fine fescue chloroplast genome phylogeny comparative genomics low input turfgrass |
| url | https://www.frontiersin.org/article/10.3389/fgene.2019.01223/full |
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