Nitric oxide in the nucleus raphe magnus modulates cutaneous blood flow in rats during hypothermia
Objective(s): Nucleus Raphe Magnus (NRM) that is involved in the regulation of body temperature contains nitric oxide (NO) synthase. Considering the effect of NO on skin blood flow control, in this study, we assessed its thermoregulatory role within the raphe magnus. Materials and Methods: To this e...
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| Format: | Article |
| Language: | English |
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Mashhad University of Medical Sciences
2015-10-01
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| Series: | Iranian Journal of Basic Medical Sciences |
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| Online Access: | http://ijbms.mums.ac.ir/pdf_5462_d312a93536a79b32a27d83c3511e8a5c.html |
| _version_ | 1819138152063827968 |
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| author | Masoumeh Kourosh Arami Javad Mirnajafi zade Alireza Komaki Mahmood Amiri Sara Mehrpooya Ali Jahanshahi Behnam Jamei |
| author_facet | Masoumeh Kourosh Arami Javad Mirnajafi zade Alireza Komaki Mahmood Amiri Sara Mehrpooya Ali Jahanshahi Behnam Jamei |
| author_sort | Masoumeh Kourosh Arami |
| collection | DOAJ |
| description | Objective(s): Nucleus Raphe Magnus (NRM) that is involved in the regulation of body temperature contains nitric oxide (NO) synthase. Considering the effect of NO on skin blood flow control, in this study, we assessed its thermoregulatory role within the raphe magnus. Materials and Methods: To this end, tail blood flow of male Wistar rats was measured by laser doppler following the induction of hypothermia. Results: Intra-NRM injection of SNP (exogenous NO donor, 0.1- 0.2 μl, 0.2 nM) increased the blood flow. Similarly, unilateral microinjection of glutamate (0.1- 0.2 μl, 2.3 nM) into the nucleus increased the blood flow. This effectof L-glutamate was reduced by prior intra NRM administrationof NO synthase inhibitor NG-methyl-L-arginine or NG-nitro-L-argininemethyl ester (L-NAME, 0.1 µl, 100 nM). Conclusion: It is concluded that NO modulates the thermoregulatory response of NRM to hypothermia and may interactwith excitatory amino acids in central skin blood flow regulation. |
| first_indexed | 2024-12-22T11:02:13Z |
| format | Article |
| id | doaj.art-2e9f03d88c96464bad14c72ef64ac0b7 |
| institution | Directory Open Access Journal |
| issn | 2008-3866 2008-3874 |
| language | English |
| last_indexed | 2024-12-22T11:02:13Z |
| publishDate | 2015-10-01 |
| publisher | Mashhad University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Basic Medical Sciences |
| spelling | doaj.art-2e9f03d88c96464bad14c72ef64ac0b72022-12-21T18:28:28ZengMashhad University of Medical SciencesIranian Journal of Basic Medical Sciences2008-38662008-38742015-10-0118109899925462Nitric oxide in the nucleus raphe magnus modulates cutaneous blood flow in rats during hypothermiaMasoumeh Kourosh Arami0Javad Mirnajafi zade1Alireza Komaki2Mahmood Amiri3Sara Mehrpooya4Ali Jahanshahi5Behnam Jamei6Department of Basic Sciences, Allied Medical College, Iran University of Medical Sciences, Tehran, Iran|Medical College of Saveh, Saveh University of Medical Sciences, Saveh, IranDepartment of Physiology, Tarbiat Modares University, Tehran, IranDepartment of Physiology, Hamedan University of Medical Sciences, Hamedan, IranDepartment of Physiology, Kermanshah University of Medical Sciences, Kermanshah, IranDepartment of Basic Sciences, Allied Medical College, Iran University of Medical Sciences, Tehran, IranDepartment of Physiology, Tarbiat Modares University, Tehran, IranDepartment of Basic Sciences, Allied Medical College, Iran University of Medical Sciences, Tehran, IranObjective(s): Nucleus Raphe Magnus (NRM) that is involved in the regulation of body temperature contains nitric oxide (NO) synthase. Considering the effect of NO on skin blood flow control, in this study, we assessed its thermoregulatory role within the raphe magnus. Materials and Methods: To this end, tail blood flow of male Wistar rats was measured by laser doppler following the induction of hypothermia. Results: Intra-NRM injection of SNP (exogenous NO donor, 0.1- 0.2 μl, 0.2 nM) increased the blood flow. Similarly, unilateral microinjection of glutamate (0.1- 0.2 μl, 2.3 nM) into the nucleus increased the blood flow. This effectof L-glutamate was reduced by prior intra NRM administrationof NO synthase inhibitor NG-methyl-L-arginine or NG-nitro-L-argininemethyl ester (L-NAME, 0.1 µl, 100 nM). Conclusion: It is concluded that NO modulates the thermoregulatory response of NRM to hypothermia and may interactwith excitatory amino acids in central skin blood flow regulation.http://ijbms.mums.ac.ir/pdf_5462_d312a93536a79b32a27d83c3511e8a5c.htmlL-NAMENitric oxideRaphe magnusSodium nitroprusside |
| spellingShingle | Masoumeh Kourosh Arami Javad Mirnajafi zade Alireza Komaki Mahmood Amiri Sara Mehrpooya Ali Jahanshahi Behnam Jamei Nitric oxide in the nucleus raphe magnus modulates cutaneous blood flow in rats during hypothermia Iranian Journal of Basic Medical Sciences L-NAME Nitric oxide Raphe magnus Sodium nitroprusside |
| title | Nitric oxide in the nucleus raphe magnus modulates cutaneous blood flow in rats during hypothermia |
| title_full | Nitric oxide in the nucleus raphe magnus modulates cutaneous blood flow in rats during hypothermia |
| title_fullStr | Nitric oxide in the nucleus raphe magnus modulates cutaneous blood flow in rats during hypothermia |
| title_full_unstemmed | Nitric oxide in the nucleus raphe magnus modulates cutaneous blood flow in rats during hypothermia |
| title_short | Nitric oxide in the nucleus raphe magnus modulates cutaneous blood flow in rats during hypothermia |
| title_sort | nitric oxide in the nucleus raphe magnus modulates cutaneous blood flow in rats during hypothermia |
| topic | L-NAME Nitric oxide Raphe magnus Sodium nitroprusside |
| url | http://ijbms.mums.ac.ir/pdf_5462_d312a93536a79b32a27d83c3511e8a5c.html |
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