The protective role of HMGB1 in affecting the balance between autophagy and pyroptosis to maintain neutrophils homeostasis during β-glucan-induced mice lung inflammation
Fungal contamination is omnipresent, and inhalation of fungi-contaminated organic dust leads to hypersensitivity pneumonitis (HP), in which neutrophils played a pivotal role. Existing studies have suggested that cell homeostasis is crucial for the pathogenesis of the inflammatory disease. Although H...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-03-01
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| Series: | Ecotoxicology and Environmental Safety |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651323001410 |
| _version_ | 1797902943565905920 |
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| author | Xinning Zeng Jiaxuan Xin Kaiyue Liu Wei Deng Fangwei Liu |
| author_facet | Xinning Zeng Jiaxuan Xin Kaiyue Liu Wei Deng Fangwei Liu |
| author_sort | Xinning Zeng |
| collection | DOAJ |
| description | Fungal contamination is omnipresent, and inhalation of fungi-contaminated organic dust leads to hypersensitivity pneumonitis (HP), in which neutrophils played a pivotal role. Existing studies have suggested that cell homeostasis is crucial for the pathogenesis of the inflammatory disease. Although HMGB1 has been shown to contribute to suppressing HP, there is a lack of studies on its mechanisms, especially the regulation of neutrophil homeostasis. This study aims to investigate how HMGB1 regulates neutrophil function by affecting neutrophil homeostasis, and then affects lung inflammation induced by β-glucan, the exposure marker of fungi. Our results showed that deficient HMGB1 led to neutrophil death by disrupting the balance between autophagy and pyroptosis after β-glucan treatment. And HMGB1 deficiency exacerbated the β-glucan-induced lung inflammation and neutrophil dysfunction both in vivo and in vitro. Furthermore, HMGB1 contributed to remodeling neutrophil function by restricting autophagy and aggravating pyroptosis β-glucan exposure. Our funding suggested that HMGB1 deficiency could break the balance between autophagy and pyroptosis towards pyroptosis to cause neutrophil dysfunction during the exacerbated inflammatory response, which provides insights into the pathogenesis of HP and the potential biological targets for its treatment. Data Availability: The datasets used during the current study are available from the corresponding author on reasonable request |
| first_indexed | 2024-04-10T09:25:11Z |
| format | Article |
| id | doaj.art-2ea5913af8b44789841378b4befbcbc1 |
| institution | Directory Open Access Journal |
| issn | 0147-6513 |
| language | English |
| last_indexed | 2024-04-10T09:25:11Z |
| publishDate | 2023-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj.art-2ea5913af8b44789841378b4befbcbc12023-02-20T04:08:46ZengElsevierEcotoxicology and Environmental Safety0147-65132023-03-01252114637The protective role of HMGB1 in affecting the balance between autophagy and pyroptosis to maintain neutrophils homeostasis during β-glucan-induced mice lung inflammationXinning Zeng0Jiaxuan Xin1Kaiyue Liu2Wei Deng3Fangwei Liu4School of Public Health, Jinzhou Medical University, Jinzhou, PR China; Division of Pneumoconiosis, School of Public Health, China Medical University, Shenyang, PR ChinaDivision of Pneumoconiosis, School of Public Health, China Medical University, Shenyang, PR ChinaDivision of Pneumoconiosis, School of Public Health, China Medical University, Shenyang, PR ChinaDivision of Pneumoconiosis, School of Public Health, China Medical University, Shenyang, PR ChinaDivision of Pneumoconiosis, School of Public Health, China Medical University, Shenyang, PR China; Corresponding author.Fungal contamination is omnipresent, and inhalation of fungi-contaminated organic dust leads to hypersensitivity pneumonitis (HP), in which neutrophils played a pivotal role. Existing studies have suggested that cell homeostasis is crucial for the pathogenesis of the inflammatory disease. Although HMGB1 has been shown to contribute to suppressing HP, there is a lack of studies on its mechanisms, especially the regulation of neutrophil homeostasis. This study aims to investigate how HMGB1 regulates neutrophil function by affecting neutrophil homeostasis, and then affects lung inflammation induced by β-glucan, the exposure marker of fungi. Our results showed that deficient HMGB1 led to neutrophil death by disrupting the balance between autophagy and pyroptosis after β-glucan treatment. And HMGB1 deficiency exacerbated the β-glucan-induced lung inflammation and neutrophil dysfunction both in vivo and in vitro. Furthermore, HMGB1 contributed to remodeling neutrophil function by restricting autophagy and aggravating pyroptosis β-glucan exposure. Our funding suggested that HMGB1 deficiency could break the balance between autophagy and pyroptosis towards pyroptosis to cause neutrophil dysfunction during the exacerbated inflammatory response, which provides insights into the pathogenesis of HP and the potential biological targets for its treatment. Data Availability: The datasets used during the current study are available from the corresponding author on reasonable requesthttp://www.sciencedirect.com/science/article/pii/S0147651323001410NeutrophilFungi contaminationAutophagyPyroptosisHypersensitivity pneumonitisβ-glucan |
| spellingShingle | Xinning Zeng Jiaxuan Xin Kaiyue Liu Wei Deng Fangwei Liu The protective role of HMGB1 in affecting the balance between autophagy and pyroptosis to maintain neutrophils homeostasis during β-glucan-induced mice lung inflammation Ecotoxicology and Environmental Safety Neutrophil Fungi contamination Autophagy Pyroptosis Hypersensitivity pneumonitis β-glucan |
| title | The protective role of HMGB1 in affecting the balance between autophagy and pyroptosis to maintain neutrophils homeostasis during β-glucan-induced mice lung inflammation |
| title_full | The protective role of HMGB1 in affecting the balance between autophagy and pyroptosis to maintain neutrophils homeostasis during β-glucan-induced mice lung inflammation |
| title_fullStr | The protective role of HMGB1 in affecting the balance between autophagy and pyroptosis to maintain neutrophils homeostasis during β-glucan-induced mice lung inflammation |
| title_full_unstemmed | The protective role of HMGB1 in affecting the balance between autophagy and pyroptosis to maintain neutrophils homeostasis during β-glucan-induced mice lung inflammation |
| title_short | The protective role of HMGB1 in affecting the balance between autophagy and pyroptosis to maintain neutrophils homeostasis during β-glucan-induced mice lung inflammation |
| title_sort | protective role of hmgb1 in affecting the balance between autophagy and pyroptosis to maintain neutrophils homeostasis during β glucan induced mice lung inflammation |
| topic | Neutrophil Fungi contamination Autophagy Pyroptosis Hypersensitivity pneumonitis β-glucan |
| url | http://www.sciencedirect.com/science/article/pii/S0147651323001410 |
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