Molecular characterization of Toxoplasma gondii Type II in sheep abortion in Sardinia, Italy
During 2009–2010, 161 tissue samples (142 placentas, 16 brains, and 3 livers) from aborted ovine fetuses on Sardinia Island, Italy, were tested for toxoplasmosis. Organs that showed a positive result by nested polymerase chain reaction (PCR) targeting the ITS1 region for Toxoplasma gondii were also...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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EDP Sciences
2014-01-01
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| Series: | Parasite |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1051/parasite/2014007 |
| _version_ | 1797427396135092224 |
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| author | Chessa Giovanna Chisu Valentina Porcu Rosaura Masala Giovanna |
| author_facet | Chessa Giovanna Chisu Valentina Porcu Rosaura Masala Giovanna |
| author_sort | Chessa Giovanna |
| collection | DOAJ |
| description | During 2009–2010, 161 tissue samples (142 placentas, 16 brains, and 3 livers) from aborted ovine fetuses on Sardinia Island, Italy, were tested for toxoplasmosis. Organs that showed a positive result by nested polymerase chain reaction (PCR) targeting the ITS1 region for Toxoplasma gondii were also amplified with 11 genetic markers (SAG1, 5′-SAG2, 3′-SAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1, and Apico) and then subjected to PCR/RFLP for genetic typing. T. gondii DNA was found in 5 placentas, 14 brains, and 2 livers by PCR analysis and all isolates displayed Type II alleles at all 11 loci with all 11 markers. The results indicate that the Type II T. gondii is associated with ovine abortion. |
| first_indexed | 2024-03-09T08:43:42Z |
| format | Article |
| id | doaj.art-2ea59e5d8d764065ac384d7798a30ba9 |
| institution | Directory Open Access Journal |
| issn | 1776-1042 |
| language | English |
| last_indexed | 2024-03-09T08:43:42Z |
| publishDate | 2014-01-01 |
| publisher | EDP Sciences |
| record_format | Article |
| series | Parasite |
| spelling | doaj.art-2ea59e5d8d764065ac384d7798a30ba92023-12-02T15:51:05ZengEDP SciencesParasite1776-10422014-01-0121610.1051/parasite/2014007parasite130111Molecular characterization of Toxoplasma gondii Type II in sheep abortion in Sardinia, ItalyChessa GiovannaChisu ValentinaPorcu RosauraMasala GiovannaDuring 2009–2010, 161 tissue samples (142 placentas, 16 brains, and 3 livers) from aborted ovine fetuses on Sardinia Island, Italy, were tested for toxoplasmosis. Organs that showed a positive result by nested polymerase chain reaction (PCR) targeting the ITS1 region for Toxoplasma gondii were also amplified with 11 genetic markers (SAG1, 5′-SAG2, 3′-SAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1, and Apico) and then subjected to PCR/RFLP for genetic typing. T. gondii DNA was found in 5 placentas, 14 brains, and 2 livers by PCR analysis and all isolates displayed Type II alleles at all 11 loci with all 11 markers. The results indicate that the Type II T. gondii is associated with ovine abortion.http://dx.doi.org/10.1051/parasite/2014007Toxoplasma gondiiGenetic characterizationSheepAbortion |
| spellingShingle | Chessa Giovanna Chisu Valentina Porcu Rosaura Masala Giovanna Molecular characterization of Toxoplasma gondii Type II in sheep abortion in Sardinia, Italy Parasite Toxoplasma gondii Genetic characterization Sheep Abortion |
| title | Molecular characterization of Toxoplasma gondii Type II in sheep abortion in Sardinia, Italy |
| title_full | Molecular characterization of Toxoplasma gondii Type II in sheep abortion in Sardinia, Italy |
| title_fullStr | Molecular characterization of Toxoplasma gondii Type II in sheep abortion in Sardinia, Italy |
| title_full_unstemmed | Molecular characterization of Toxoplasma gondii Type II in sheep abortion in Sardinia, Italy |
| title_short | Molecular characterization of Toxoplasma gondii Type II in sheep abortion in Sardinia, Italy |
| title_sort | molecular characterization of toxoplasma gondii type ii in sheep abortion in sardinia italy |
| topic | Toxoplasma gondii Genetic characterization Sheep Abortion |
| url | http://dx.doi.org/10.1051/parasite/2014007 |
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