Stool metabolome-microbiota evaluation among children and adolescents with obesity, overweight, and normal-weight using 1H NMR and 16S rRNA gene profiling.
Characterization of metabolites and microbiota composition from human stool provides powerful insight into the molecular phenotypic difference between subjects with normal weight and those with overweight/obesity. The aim of this study was to identify potential metabolic and bacterial signatures fro...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2021-01-01
|
| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0247378 |
| _version_ | 1818891696890445824 |
|---|---|
| author | José Diógenes Jaimes Andrea Slavíčková Jakub Hurych Ondřej Cinek Ben Nichols Lucie Vodolánová Karel Černý Jaroslav Havlík |
| author_facet | José Diógenes Jaimes Andrea Slavíčková Jakub Hurych Ondřej Cinek Ben Nichols Lucie Vodolánová Karel Černý Jaroslav Havlík |
| author_sort | José Diógenes Jaimes |
| collection | DOAJ |
| description | Characterization of metabolites and microbiota composition from human stool provides powerful insight into the molecular phenotypic difference between subjects with normal weight and those with overweight/obesity. The aim of this study was to identify potential metabolic and bacterial signatures from stool that distinguish the overweight/obesity state in children/adolescents. Using 1H NMR spectral analysis and 16S rRNA gene profiling, the fecal metabolic profile and bacterial composition from 52 children aged 7 to 16 was evaluated. The children were classified into three groups (16 with normal-weight, 17 with overweight, 19 with obesity). The metabolomic analysis identified four metabolites that were significantly different (p < 0.05) among the study groups based on one-way ANOVA testing: arabinose, butyrate, galactose, and trimethylamine. Significantly different (p < 0.01) genus-level taxa based on edgeR differential abundance tests were genus Escherichia and Tyzzerella subgroup 3. No significant difference in alpha-diversity was detected among the three study groups, and no significant correlations were found between the significant taxa and metabolites. The findings support the hypothesis of increased energy harvest in obesity by human gut bacteria through the growing observation of increased fecal butyrate in children with overweight/obesity, as well as an increase of certain monosaccharides in the stool. Also supported is the increase of trimethylamine as an indicator of an unhealthy state. |
| first_indexed | 2024-12-19T17:44:55Z |
| format | Article |
| id | doaj.art-2eaa37a78e6f4e36b12216ae5da9f91c |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-19T17:44:55Z |
| publishDate | 2021-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-2eaa37a78e6f4e36b12216ae5da9f91c2022-12-21T20:12:05ZengPublic Library of Science (PLoS)PLoS ONE1932-62032021-01-01163e024737810.1371/journal.pone.0247378Stool metabolome-microbiota evaluation among children and adolescents with obesity, overweight, and normal-weight using 1H NMR and 16S rRNA gene profiling.José Diógenes JaimesAndrea SlavíčkováJakub HurychOndřej CinekBen NicholsLucie VodolánováKarel ČernýJaroslav HavlíkCharacterization of metabolites and microbiota composition from human stool provides powerful insight into the molecular phenotypic difference between subjects with normal weight and those with overweight/obesity. The aim of this study was to identify potential metabolic and bacterial signatures from stool that distinguish the overweight/obesity state in children/adolescents. Using 1H NMR spectral analysis and 16S rRNA gene profiling, the fecal metabolic profile and bacterial composition from 52 children aged 7 to 16 was evaluated. The children were classified into three groups (16 with normal-weight, 17 with overweight, 19 with obesity). The metabolomic analysis identified four metabolites that were significantly different (p < 0.05) among the study groups based on one-way ANOVA testing: arabinose, butyrate, galactose, and trimethylamine. Significantly different (p < 0.01) genus-level taxa based on edgeR differential abundance tests were genus Escherichia and Tyzzerella subgroup 3. No significant difference in alpha-diversity was detected among the three study groups, and no significant correlations were found between the significant taxa and metabolites. The findings support the hypothesis of increased energy harvest in obesity by human gut bacteria through the growing observation of increased fecal butyrate in children with overweight/obesity, as well as an increase of certain monosaccharides in the stool. Also supported is the increase of trimethylamine as an indicator of an unhealthy state.https://doi.org/10.1371/journal.pone.0247378 |
| spellingShingle | José Diógenes Jaimes Andrea Slavíčková Jakub Hurych Ondřej Cinek Ben Nichols Lucie Vodolánová Karel Černý Jaroslav Havlík Stool metabolome-microbiota evaluation among children and adolescents with obesity, overweight, and normal-weight using 1H NMR and 16S rRNA gene profiling. PLoS ONE |
| title | Stool metabolome-microbiota evaluation among children and adolescents with obesity, overweight, and normal-weight using 1H NMR and 16S rRNA gene profiling. |
| title_full | Stool metabolome-microbiota evaluation among children and adolescents with obesity, overweight, and normal-weight using 1H NMR and 16S rRNA gene profiling. |
| title_fullStr | Stool metabolome-microbiota evaluation among children and adolescents with obesity, overweight, and normal-weight using 1H NMR and 16S rRNA gene profiling. |
| title_full_unstemmed | Stool metabolome-microbiota evaluation among children and adolescents with obesity, overweight, and normal-weight using 1H NMR and 16S rRNA gene profiling. |
| title_short | Stool metabolome-microbiota evaluation among children and adolescents with obesity, overweight, and normal-weight using 1H NMR and 16S rRNA gene profiling. |
| title_sort | stool metabolome microbiota evaluation among children and adolescents with obesity overweight and normal weight using 1h nmr and 16s rrna gene profiling |
| url | https://doi.org/10.1371/journal.pone.0247378 |
| work_keys_str_mv | AT josediogenesjaimes stoolmetabolomemicrobiotaevaluationamongchildrenandadolescentswithobesityoverweightandnormalweightusing1hnmrand16srrnageneprofiling AT andreaslavickova stoolmetabolomemicrobiotaevaluationamongchildrenandadolescentswithobesityoverweightandnormalweightusing1hnmrand16srrnageneprofiling AT jakubhurych stoolmetabolomemicrobiotaevaluationamongchildrenandadolescentswithobesityoverweightandnormalweightusing1hnmrand16srrnageneprofiling AT ondrejcinek stoolmetabolomemicrobiotaevaluationamongchildrenandadolescentswithobesityoverweightandnormalweightusing1hnmrand16srrnageneprofiling AT bennichols stoolmetabolomemicrobiotaevaluationamongchildrenandadolescentswithobesityoverweightandnormalweightusing1hnmrand16srrnageneprofiling AT lucievodolanova stoolmetabolomemicrobiotaevaluationamongchildrenandadolescentswithobesityoverweightandnormalweightusing1hnmrand16srrnageneprofiling AT karelcerny stoolmetabolomemicrobiotaevaluationamongchildrenandadolescentswithobesityoverweightandnormalweightusing1hnmrand16srrnageneprofiling AT jaroslavhavlik stoolmetabolomemicrobiotaevaluationamongchildrenandadolescentswithobesityoverweightandnormalweightusing1hnmrand16srrnageneprofiling |