Chitosan from Crabs (<i>Scylla serrata</i>) Represses Hyperlipidemia-Induced Hepato-Renal Dysfunctions in Rats: Modulation of CD43 and p53 Expression
Hepato-renal dysfunctions associated with hyperlipidemia necessitates a continuous search for natural remedies. This study thus evaluated the effect of dietary chitosan on diet-induced hyperlipidemia in rats. A total of 30 male Wistar rats (90 ± 10) g were randomly allotted into six (6) groups (<...
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MDPI AG
2021-05-01
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| Series: | Pathophysiology |
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| Online Access: | https://www.mdpi.com/1873-149X/28/2/15 |
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| author | Regina Ngozi Ugbaja Kunle Ogungbemi Adewale Segun James Ayodele Peter Folorunsho Samuel Olanrewaju Abolade Stella Onajite Ajamikoko Eniola Olapeju Atayese Omowunmi Victoria Adedeji |
| author_facet | Regina Ngozi Ugbaja Kunle Ogungbemi Adewale Segun James Ayodele Peter Folorunsho Samuel Olanrewaju Abolade Stella Onajite Ajamikoko Eniola Olapeju Atayese Omowunmi Victoria Adedeji |
| author_sort | Regina Ngozi Ugbaja |
| collection | DOAJ |
| description | Hepato-renal dysfunctions associated with hyperlipidemia necessitates a continuous search for natural remedies. This study thus evaluated the effect of dietary chitosan on diet-induced hyperlipidemia in rats. A total of 30 male Wistar rats (90 ± 10) g were randomly allotted into six (6) groups (<i>n</i> = 5): Normal diet, High-fat diet (HFD), and Normal diet + 5% chitosan. The three other groups received HFD, supplemented with 1%, 3%, and 5% of chitosan. The feeding lasted for 6 weeks, after which the rats were sacrificed. The liver and kidneys were harvested for analyses. Hepatic alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP) activity, and renal biomarkers (ALT, AST, urea, and creatinine) were assayed spectrophotometrically. Additionally, expression of hepatic and renal CD43 and p53 was estimated immunohistochemically. The HFD group had elevated bodyweight compared to normal which was reversed in the chitosan-supplemented groups. Hyperlipidemia caused a significant (<i>p</i> < 0.05) decrease in the hepatic (AST, ALT, and ALP) and renal (AST and ALT) activities, while renal urea and creatinine increased. Furthermore, the HFD group showed an elevated level of hepatic and renal CD43 while p53 expression decreased. However, groups supplemented with chitosan showed improved hepatic and renal biomarkers, as well as corrected the aberrations in the expressions of p53 and CD43. Conclusively, dietary chitosan inclusion in the diet (between 3% and 5%) could effectively improve kidney and liver functionality via abatement of inflammatory responses. |
| first_indexed | 2024-03-10T07:59:50Z |
| format | Article |
| id | doaj.art-2eab2bb9a9014d3ba17a41fc47de8ca8 |
| institution | Directory Open Access Journal |
| issn | 1873-149X |
| language | English |
| last_indexed | 2024-03-10T07:59:50Z |
| publishDate | 2021-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pathophysiology |
| spelling | doaj.art-2eab2bb9a9014d3ba17a41fc47de8ca82023-11-22T11:31:59ZengMDPI AGPathophysiology1873-149X2021-05-0128222423710.3390/pathophysiology28020015Chitosan from Crabs (<i>Scylla serrata</i>) Represses Hyperlipidemia-Induced Hepato-Renal Dysfunctions in Rats: Modulation of CD43 and p53 ExpressionRegina Ngozi Ugbaja0Kunle Ogungbemi1Adewale Segun James2Ayodele Peter Folorunsho3Samuel Olanrewaju Abolade4Stella Onajite Ajamikoko5Eniola Olapeju Atayese6Omowunmi Victoria Adedeji7Department of Biochemistry, College of Bioscience, Federal University of Agriculture, P.M.B. 2240 Abeokuta, NigeriaDepartment of Biochemistry, College of Bioscience, Federal University of Agriculture, P.M.B. 2240 Abeokuta, NigeriaDepartment of Biochemistry, College of Bioscience, Federal University of Agriculture, P.M.B. 2240 Abeokuta, NigeriaDepartment of Biochemistry, College of Bioscience, Federal University of Agriculture, P.M.B. 2240 Abeokuta, NigeriaDepartment of Biochemistry, College of Bioscience, Federal University of Agriculture, P.M.B. 2240 Abeokuta, NigeriaDepartment of Biochemistry, College of Bioscience, Federal University of Agriculture, P.M.B. 2240 Abeokuta, NigeriaDepartment of Biochemistry, College of Bioscience, Federal University of Agriculture, P.M.B. 2240 Abeokuta, NigeriaDepartment of Biochemistry, College of Bioscience, Federal University of Agriculture, P.M.B. 2240 Abeokuta, NigeriaHepato-renal dysfunctions associated with hyperlipidemia necessitates a continuous search for natural remedies. This study thus evaluated the effect of dietary chitosan on diet-induced hyperlipidemia in rats. A total of 30 male Wistar rats (90 ± 10) g were randomly allotted into six (6) groups (<i>n</i> = 5): Normal diet, High-fat diet (HFD), and Normal diet + 5% chitosan. The three other groups received HFD, supplemented with 1%, 3%, and 5% of chitosan. The feeding lasted for 6 weeks, after which the rats were sacrificed. The liver and kidneys were harvested for analyses. Hepatic alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP) activity, and renal biomarkers (ALT, AST, urea, and creatinine) were assayed spectrophotometrically. Additionally, expression of hepatic and renal CD43 and p53 was estimated immunohistochemically. The HFD group had elevated bodyweight compared to normal which was reversed in the chitosan-supplemented groups. Hyperlipidemia caused a significant (<i>p</i> < 0.05) decrease in the hepatic (AST, ALT, and ALP) and renal (AST and ALT) activities, while renal urea and creatinine increased. Furthermore, the HFD group showed an elevated level of hepatic and renal CD43 while p53 expression decreased. However, groups supplemented with chitosan showed improved hepatic and renal biomarkers, as well as corrected the aberrations in the expressions of p53 and CD43. Conclusively, dietary chitosan inclusion in the diet (between 3% and 5%) could effectively improve kidney and liver functionality via abatement of inflammatory responses.https://www.mdpi.com/1873-149X/28/2/15chitosanhyperlipidemiahigh-fat dietp53 and CD43 genesfunctional indices |
| spellingShingle | Regina Ngozi Ugbaja Kunle Ogungbemi Adewale Segun James Ayodele Peter Folorunsho Samuel Olanrewaju Abolade Stella Onajite Ajamikoko Eniola Olapeju Atayese Omowunmi Victoria Adedeji Chitosan from Crabs (<i>Scylla serrata</i>) Represses Hyperlipidemia-Induced Hepato-Renal Dysfunctions in Rats: Modulation of CD43 and p53 Expression Pathophysiology chitosan hyperlipidemia high-fat diet p53 and CD43 genes functional indices |
| title | Chitosan from Crabs (<i>Scylla serrata</i>) Represses Hyperlipidemia-Induced Hepato-Renal Dysfunctions in Rats: Modulation of CD43 and p53 Expression |
| title_full | Chitosan from Crabs (<i>Scylla serrata</i>) Represses Hyperlipidemia-Induced Hepato-Renal Dysfunctions in Rats: Modulation of CD43 and p53 Expression |
| title_fullStr | Chitosan from Crabs (<i>Scylla serrata</i>) Represses Hyperlipidemia-Induced Hepato-Renal Dysfunctions in Rats: Modulation of CD43 and p53 Expression |
| title_full_unstemmed | Chitosan from Crabs (<i>Scylla serrata</i>) Represses Hyperlipidemia-Induced Hepato-Renal Dysfunctions in Rats: Modulation of CD43 and p53 Expression |
| title_short | Chitosan from Crabs (<i>Scylla serrata</i>) Represses Hyperlipidemia-Induced Hepato-Renal Dysfunctions in Rats: Modulation of CD43 and p53 Expression |
| title_sort | chitosan from crabs i scylla serrata i represses hyperlipidemia induced hepato renal dysfunctions in rats modulation of cd43 and p53 expression |
| topic | chitosan hyperlipidemia high-fat diet p53 and CD43 genes functional indices |
| url | https://www.mdpi.com/1873-149X/28/2/15 |
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