Effects of knockout of CO2-responsive CCT protein, a regulator of starch synthesis on physiological characteristics of rice
ABSTRACTCO2-Responsive CCT Protein (CRCT) is suggested to be a positive regulator of starch synthesis-related genes in the vegetative organs of rice. To investigate the physiological function of CRCT, we produced CRCT knockout lines by CRISPR/Cas9 system. CRCT knockout tended to slightly reduce phot...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2023-07-01
|
| Series: | Plant Production Science |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/1343943X.2023.2233161 |
| _version_ | 1797745036025135104 |
|---|---|
| author | Fumihiro Miyagawa Naoki Shibatani Ryutaro Morita Daisuke Sasayama Tomoko Hatanaka Tetsushi Azuma Hiroshi Fukayama |
| author_facet | Fumihiro Miyagawa Naoki Shibatani Ryutaro Morita Daisuke Sasayama Tomoko Hatanaka Tetsushi Azuma Hiroshi Fukayama |
| author_sort | Fumihiro Miyagawa |
| collection | DOAJ |
| description | ABSTRACTCO2-Responsive CCT Protein (CRCT) is suggested to be a positive regulator of starch synthesis-related genes in the vegetative organs of rice. To investigate the physiological function of CRCT, we produced CRCT knockout lines by CRISPR/Cas9 system. CRCT knockout tended to slightly reduce photosynthetic performance and had some adverse effects on growth parameters such as tiller number and straw dry weight. The starch content in the leaf sheath of CRCT knockout lines was significantly lower than that of wild-type. qRT-PCR and RNA-seq analysis showed that sucrose treatment induced the expression of CRCT, which, in turn, induced many starch synthesis-related genes in wild-type, whereas this induction did not occur in CRCT knockout lines. In conclusion, our results suggest that sugar induction of starch synthesis-related genes is almost fully dependent on CRCT, making it a necessary factor for normal photosynthesis and growth of rice. |
| first_indexed | 2024-03-12T15:17:44Z |
| format | Article |
| id | doaj.art-2eabe375aa1347a3a379d6036ac96671 |
| institution | Directory Open Access Journal |
| issn | 1343-943X 1349-1008 |
| language | English |
| last_indexed | 2024-03-12T15:17:44Z |
| publishDate | 2023-07-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Plant Production Science |
| spelling | doaj.art-2eabe375aa1347a3a379d6036ac966712023-08-11T11:04:44ZengTaylor & Francis GroupPlant Production Science1343-943X1349-10082023-07-0126327328610.1080/1343943X.2023.2233161Effects of knockout of CO2-responsive CCT protein, a regulator of starch synthesis on physiological characteristics of riceFumihiro Miyagawa0Naoki Shibatani1Ryutaro Morita2Daisuke Sasayama3Tomoko Hatanaka4Tetsushi Azuma5Hiroshi Fukayama6Laboratory of Tropical Crop Science, Graduate School of Agricultural Science, Kobe University, Kobe, JapanLaboratory of Tropical Crop Science, Graduate School of Agricultural Science, Kobe University, Kobe, JapanLaboratory of Tropical Crop Science, Graduate School of Agricultural Science, Kobe University, Kobe, JapanLaboratory of Tropical Crop Science, Graduate School of Agricultural Science, Kobe University, Kobe, JapanLaboratory of Tropical Crop Science, Graduate School of Agricultural Science, Kobe University, Kobe, JapanLaboratory of Tropical Crop Science, Graduate School of Agricultural Science, Kobe University, Kobe, JapanLaboratory of Tropical Crop Science, Graduate School of Agricultural Science, Kobe University, Kobe, JapanABSTRACTCO2-Responsive CCT Protein (CRCT) is suggested to be a positive regulator of starch synthesis-related genes in the vegetative organs of rice. To investigate the physiological function of CRCT, we produced CRCT knockout lines by CRISPR/Cas9 system. CRCT knockout tended to slightly reduce photosynthetic performance and had some adverse effects on growth parameters such as tiller number and straw dry weight. The starch content in the leaf sheath of CRCT knockout lines was significantly lower than that of wild-type. qRT-PCR and RNA-seq analysis showed that sucrose treatment induced the expression of CRCT, which, in turn, induced many starch synthesis-related genes in wild-type, whereas this induction did not occur in CRCT knockout lines. In conclusion, our results suggest that sugar induction of starch synthesis-related genes is almost fully dependent on CRCT, making it a necessary factor for normal photosynthesis and growth of rice.https://www.tandfonline.com/doi/10.1080/1343943X.2023.2233161CCT ProteingrowthphotosynthesisRice (oryza sativa)sugar responsestarch |
| spellingShingle | Fumihiro Miyagawa Naoki Shibatani Ryutaro Morita Daisuke Sasayama Tomoko Hatanaka Tetsushi Azuma Hiroshi Fukayama Effects of knockout of CO2-responsive CCT protein, a regulator of starch synthesis on physiological characteristics of rice Plant Production Science CCT Protein growth photosynthesis Rice (oryza sativa) sugar response starch |
| title | Effects of knockout of CO2-responsive CCT protein, a regulator of starch synthesis on physiological characteristics of rice |
| title_full | Effects of knockout of CO2-responsive CCT protein, a regulator of starch synthesis on physiological characteristics of rice |
| title_fullStr | Effects of knockout of CO2-responsive CCT protein, a regulator of starch synthesis on physiological characteristics of rice |
| title_full_unstemmed | Effects of knockout of CO2-responsive CCT protein, a regulator of starch synthesis on physiological characteristics of rice |
| title_short | Effects of knockout of CO2-responsive CCT protein, a regulator of starch synthesis on physiological characteristics of rice |
| title_sort | effects of knockout of co2 responsive cct protein a regulator of starch synthesis on physiological characteristics of rice |
| topic | CCT Protein growth photosynthesis Rice (oryza sativa) sugar response starch |
| url | https://www.tandfonline.com/doi/10.1080/1343943X.2023.2233161 |
| work_keys_str_mv | AT fumihiromiyagawa effectsofknockoutofco2responsivecctproteinaregulatorofstarchsynthesisonphysiologicalcharacteristicsofrice AT naokishibatani effectsofknockoutofco2responsivecctproteinaregulatorofstarchsynthesisonphysiologicalcharacteristicsofrice AT ryutaromorita effectsofknockoutofco2responsivecctproteinaregulatorofstarchsynthesisonphysiologicalcharacteristicsofrice AT daisukesasayama effectsofknockoutofco2responsivecctproteinaregulatorofstarchsynthesisonphysiologicalcharacteristicsofrice AT tomokohatanaka effectsofknockoutofco2responsivecctproteinaregulatorofstarchsynthesisonphysiologicalcharacteristicsofrice AT tetsushiazuma effectsofknockoutofco2responsivecctproteinaregulatorofstarchsynthesisonphysiologicalcharacteristicsofrice AT hiroshifukayama effectsofknockoutofco2responsivecctproteinaregulatorofstarchsynthesisonphysiologicalcharacteristicsofrice |