Eucalyptus Globulus protects against diclofenac sodium induced hepatorenal and testicular toxicity in male rats
The current study was conducted to investigate the protective properties of Eucalyptus globulus leaves methanolic extract (EGLME) against diclofenac sodium (DS) induced hepatorenal and testicular toxicity in male rats. A total of 40 rats were equally divided into 4 groups, Control, Diclofenac sodium...
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| Format: | Article |
| Language: | English |
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Elsevier
2020-11-01
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| Series: | Journal of Traditional and Complementary Medicine |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2225411019304365 |
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| author | Ahmed Abdelmoniem Mousa Ahmed Essam Elweza Hamed Talaat Elbaz Enas Abd El-aziz Tahoun Khaled Mohamed Shoghy Islam Elsayed El Barbary Hassan |
| author_facet | Ahmed Abdelmoniem Mousa Ahmed Essam Elweza Hamed Talaat Elbaz Enas Abd El-aziz Tahoun Khaled Mohamed Shoghy Islam Elsayed El Barbary Hassan |
| author_sort | Ahmed Abdelmoniem Mousa |
| collection | DOAJ |
| description | The current study was conducted to investigate the protective properties of Eucalyptus globulus leaves methanolic extract (EGLME) against diclofenac sodium (DS) induced hepatorenal and testicular toxicity in male rats. A total of 40 rats were equally divided into 4 groups, Control, Diclofenac sodium (DS), EGLME and DS + EGLME groups, respectively. DS and EGLME were administered orally at dose rate 2.5 and 100 mg/kg BW, 4 times/week for 8 weeks, respectively. Administration of DS distorted hepatorenal functions manifested by alteration of serum levels of ALT, AST, total protein and albumin, creatinine and urea with changes of histological architectures. DS caused reproductive toxicity represented by minimized sperm count, individual sperm motility and viability; depleted concentration of reduced glutathione (GSH) in testicular tissue; and decreased testosterone level with alteration in testicular histological features. In contrast, co-treatment of DS intoxicated rats with EGLME protected rats against the adverse effects of DS revealing enhancing properties of EGLME on rats’ liver, kidney and testes. In conclusion, we demonstrated that EGLME had a potent protecting property against DS induced hepatic, renal and testicular toxicity in male rats, with special concern to testicular tissue via modulation of GSH as an oxidant marker. Taxonomy: (classification by EVISE): Diclofenac sodium toxicity (hepatorenal and testicular toxicity), co-treatment with natural herbal extract, blood biochemical assays, tissue anti-oxidants assay, histopathology and reproductive indices analyses. |
| first_indexed | 2024-12-12T02:12:56Z |
| format | Article |
| id | doaj.art-2eae7c0698584968ba33d4e76fb852cd |
| institution | Directory Open Access Journal |
| issn | 2225-4110 |
| language | English |
| last_indexed | 2024-12-12T02:12:56Z |
| publishDate | 2020-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Traditional and Complementary Medicine |
| spelling | doaj.art-2eae7c0698584968ba33d4e76fb852cd2022-12-22T00:41:52ZengElsevierJournal of Traditional and Complementary Medicine2225-41102020-11-01106521528Eucalyptus Globulus protects against diclofenac sodium induced hepatorenal and testicular toxicity in male ratsAhmed Abdelmoniem Mousa0Ahmed Essam Elweza1Hamed Talaat Elbaz2Enas Abd El-aziz Tahoun3Khaled Mohamed Shoghy4Islam Elsayed5El Barbary Hassan6Department of Biochemistry and Chemistry of Nutrition, Faculty of Veterinary Medicine, University of Sadat City, Sadat City, 32897, Menoufia, Egypt; Corresponding author. Department of Biochemistry and Chemistry of Nutrition, Faculty of Veterinary Medicine, University of Sadat City, Sadat City, 32897, Egypt.Department of Theriogenology, Faculty of Veterinary Medicine, University of Sadat City, Sadat City, 32897, Menoufia, EgyptDepartment of Theriogenology, Faculty of Veterinary Medicine, University of Sadat City, Sadat City, 32897, Menoufia, EgyptDepartment of Pathology, Faculty of Veterinary Medicine, University of Sadat City, Sadat City, 32897, Menoufia, EgyptDepartment of Anatomy and Embryology, Faculty of Veterinary Medicine, University of Sadat City, Sadat, City, 32897, Menoufia, EgyptDepartment of Sustainable Bioproducts, Mississippi State University, Box 9820, Mississippi State, MS, 39762, United StatesDepartment of Sustainable Bioproducts, Mississippi State University, Box 9820, Mississippi State, MS, 39762, United StatesThe current study was conducted to investigate the protective properties of Eucalyptus globulus leaves methanolic extract (EGLME) against diclofenac sodium (DS) induced hepatorenal and testicular toxicity in male rats. A total of 40 rats were equally divided into 4 groups, Control, Diclofenac sodium (DS), EGLME and DS + EGLME groups, respectively. DS and EGLME were administered orally at dose rate 2.5 and 100 mg/kg BW, 4 times/week for 8 weeks, respectively. Administration of DS distorted hepatorenal functions manifested by alteration of serum levels of ALT, AST, total protein and albumin, creatinine and urea with changes of histological architectures. DS caused reproductive toxicity represented by minimized sperm count, individual sperm motility and viability; depleted concentration of reduced glutathione (GSH) in testicular tissue; and decreased testosterone level with alteration in testicular histological features. In contrast, co-treatment of DS intoxicated rats with EGLME protected rats against the adverse effects of DS revealing enhancing properties of EGLME on rats’ liver, kidney and testes. In conclusion, we demonstrated that EGLME had a potent protecting property against DS induced hepatic, renal and testicular toxicity in male rats, with special concern to testicular tissue via modulation of GSH as an oxidant marker. Taxonomy: (classification by EVISE): Diclofenac sodium toxicity (hepatorenal and testicular toxicity), co-treatment with natural herbal extract, blood biochemical assays, tissue anti-oxidants assay, histopathology and reproductive indices analyses.http://www.sciencedirect.com/science/article/pii/S2225411019304365Eucalyptus globulusLiverKidneyReproductive toxicityReduced glutathione |
| spellingShingle | Ahmed Abdelmoniem Mousa Ahmed Essam Elweza Hamed Talaat Elbaz Enas Abd El-aziz Tahoun Khaled Mohamed Shoghy Islam Elsayed El Barbary Hassan Eucalyptus Globulus protects against diclofenac sodium induced hepatorenal and testicular toxicity in male rats Journal of Traditional and Complementary Medicine Eucalyptus globulus Liver Kidney Reproductive toxicity Reduced glutathione |
| title | Eucalyptus Globulus protects against diclofenac sodium induced hepatorenal and testicular toxicity in male rats |
| title_full | Eucalyptus Globulus protects against diclofenac sodium induced hepatorenal and testicular toxicity in male rats |
| title_fullStr | Eucalyptus Globulus protects against diclofenac sodium induced hepatorenal and testicular toxicity in male rats |
| title_full_unstemmed | Eucalyptus Globulus protects against diclofenac sodium induced hepatorenal and testicular toxicity in male rats |
| title_short | Eucalyptus Globulus protects against diclofenac sodium induced hepatorenal and testicular toxicity in male rats |
| title_sort | eucalyptus globulus protects against diclofenac sodium induced hepatorenal and testicular toxicity in male rats |
| topic | Eucalyptus globulus Liver Kidney Reproductive toxicity Reduced glutathione |
| url | http://www.sciencedirect.com/science/article/pii/S2225411019304365 |
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