Efficacy of double-stranded RNA against white spot syndrome virus (WSSV) non-structural (orf89, wsv191) and structural (vp28, vp26) genes in the Pacific white shrimp Litopenaeus vannamei
White spot syndrome virus (WSSV) is a major pathogen in shrimp aquaculture. RNA interference (RNAi) is a promising tool against viral infections. Previous works with RNAi showed different antiviral efficacies depending on the silenced gene. This work evaluated the antiviral efficacy of double-strand...
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| Format: | Article |
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Elsevier
2015-04-01
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| Series: | Journal of King Saud University: Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1018364714000834 |
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| author | César M. Escobedo-Bonilla Sergio Vega-Peña Claudio Humberto Mejía-Ruiz |
| author_facet | César M. Escobedo-Bonilla Sergio Vega-Peña Claudio Humberto Mejía-Ruiz |
| author_sort | César M. Escobedo-Bonilla |
| collection | DOAJ |
| description | White spot syndrome virus (WSSV) is a major pathogen in shrimp aquaculture. RNA interference (RNAi) is a promising tool against viral infections. Previous works with RNAi showed different antiviral efficacies depending on the silenced gene. This work evaluated the antiviral efficacy of double-stranded (ds) RNA against two non-structural (orf89, wsv191) WSSV genes compared to structural (vp26, vp28) genes to inhibit an experimental WSSV infection. Gene orf89 encodes a putative regulatory protein and gene white spot virus (wsv)191 encodes a nonspecific nuclease; whereas genes vp26 and vp28 encode envelope proteins, respectively. Molecules of dsRNA against each of the WSSV genes were intramuscularly injected (4 μg per shrimp) into a group of shrimp 48 h before a WSSV challenge. The highest antiviral activity occurred with dsRNA against orf89, vp28 and vp26 (cumulative mortalities 10%, 10% and 21%, respectively). In contrast, the least effective treatment was wsv191 dsRNA (cumulative mortality 83%). All dead animals were WSSV-positive by one-step PCR, whereas reverse-transcription PCR of all surviving shrimp confirmed inhibition of virus replication. This study showed that dsRNA against WSSV genes orf89, vp28 and vp26 were highly effective to inhibit virus replication and suggest an essential role in WSSV infection. Non-structural WSSV genes such as orf89 can be used as novel targets to design therapeutic RNAi molecules against WSSV infection. |
| first_indexed | 2024-12-13T04:48:05Z |
| format | Article |
| id | doaj.art-2eb02a303b9542bbaa3211703907f287 |
| institution | Directory Open Access Journal |
| issn | 1018-3647 |
| language | English |
| last_indexed | 2024-12-13T04:48:05Z |
| publishDate | 2015-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of King Saud University: Science |
| spelling | doaj.art-2eb02a303b9542bbaa3211703907f2872022-12-21T23:59:05ZengElsevierJournal of King Saud University: Science1018-36472015-04-0127218218810.1016/j.jksus.2014.11.004Efficacy of double-stranded RNA against white spot syndrome virus (WSSV) non-structural (orf89, wsv191) and structural (vp28, vp26) genes in the Pacific white shrimp Litopenaeus vannameiCésar M. Escobedo-Bonilla0Sergio Vega-Peña1Claudio Humberto Mejía-Ruiz2Instituto Politecnico Nacional-CIIDIR Unidad Sinaloa, Blvd. Juan de Dios Batiz Paredes no. 250, Colonia San Joachin, Guasave, Sinaloa 81101, MexicoUniversidad Autónoma de Baja California Sur (UABCS), Carretera al Sur km 5.5, Colonia Expropiación Petrolera s/n, La Paz, B.C.S. 23080, MexicoCentro de Investigaciones Biológicas del Noroeste (CIBNOR), Mar Bermejo 195, Colonia Playa Palo de Santa Rita, La Paz, B.C.S. 23096, MexicoWhite spot syndrome virus (WSSV) is a major pathogen in shrimp aquaculture. RNA interference (RNAi) is a promising tool against viral infections. Previous works with RNAi showed different antiviral efficacies depending on the silenced gene. This work evaluated the antiviral efficacy of double-stranded (ds) RNA against two non-structural (orf89, wsv191) WSSV genes compared to structural (vp26, vp28) genes to inhibit an experimental WSSV infection. Gene orf89 encodes a putative regulatory protein and gene white spot virus (wsv)191 encodes a nonspecific nuclease; whereas genes vp26 and vp28 encode envelope proteins, respectively. Molecules of dsRNA against each of the WSSV genes were intramuscularly injected (4 μg per shrimp) into a group of shrimp 48 h before a WSSV challenge. The highest antiviral activity occurred with dsRNA against orf89, vp28 and vp26 (cumulative mortalities 10%, 10% and 21%, respectively). In contrast, the least effective treatment was wsv191 dsRNA (cumulative mortality 83%). All dead animals were WSSV-positive by one-step PCR, whereas reverse-transcription PCR of all surviving shrimp confirmed inhibition of virus replication. This study showed that dsRNA against WSSV genes orf89, vp28 and vp26 were highly effective to inhibit virus replication and suggest an essential role in WSSV infection. Non-structural WSSV genes such as orf89 can be used as novel targets to design therapeutic RNAi molecules against WSSV infection.http://www.sciencedirect.com/science/article/pii/S1018364714000834WSSVAquatic diseasesNon-structural genesdsRNAAntiviral therapeuticsLitopenaeus vannamei |
| spellingShingle | César M. Escobedo-Bonilla Sergio Vega-Peña Claudio Humberto Mejía-Ruiz Efficacy of double-stranded RNA against white spot syndrome virus (WSSV) non-structural (orf89, wsv191) and structural (vp28, vp26) genes in the Pacific white shrimp Litopenaeus vannamei Journal of King Saud University: Science WSSV Aquatic diseases Non-structural genes dsRNA Antiviral therapeutics Litopenaeus vannamei |
| title | Efficacy of double-stranded RNA against white spot syndrome virus (WSSV) non-structural (orf89, wsv191) and structural (vp28, vp26) genes in the Pacific white shrimp Litopenaeus vannamei |
| title_full | Efficacy of double-stranded RNA against white spot syndrome virus (WSSV) non-structural (orf89, wsv191) and structural (vp28, vp26) genes in the Pacific white shrimp Litopenaeus vannamei |
| title_fullStr | Efficacy of double-stranded RNA against white spot syndrome virus (WSSV) non-structural (orf89, wsv191) and structural (vp28, vp26) genes in the Pacific white shrimp Litopenaeus vannamei |
| title_full_unstemmed | Efficacy of double-stranded RNA against white spot syndrome virus (WSSV) non-structural (orf89, wsv191) and structural (vp28, vp26) genes in the Pacific white shrimp Litopenaeus vannamei |
| title_short | Efficacy of double-stranded RNA against white spot syndrome virus (WSSV) non-structural (orf89, wsv191) and structural (vp28, vp26) genes in the Pacific white shrimp Litopenaeus vannamei |
| title_sort | efficacy of double stranded rna against white spot syndrome virus wssv non structural orf89 wsv191 and structural vp28 vp26 genes in the pacific white shrimp litopenaeus vannamei |
| topic | WSSV Aquatic diseases Non-structural genes dsRNA Antiviral therapeutics Litopenaeus vannamei |
| url | http://www.sciencedirect.com/science/article/pii/S1018364714000834 |
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