Generation of mouse hippocampal brain organoids from primary embryonic neural stem cells
Summary: Here we present a protocol to generate standardized cerebral organoids with hippocampal regional specification using morphogen WNT3a. We describe steps for isolating mouse embryonic (E14.5) neural stem cells from the brain subgranular zone, preparing organoids samples for immunofluorescence...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-09-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166723003805 |
| _version_ | 1827900136068481024 |
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| author | Francesca Ciarpella Raluca Georgiana Zamfir Alessandra Campanelli Giulia Pedrotti Marzia Di Chio Emanuela Bottani Ilaria Decimo |
| author_facet | Francesca Ciarpella Raluca Georgiana Zamfir Alessandra Campanelli Giulia Pedrotti Marzia Di Chio Emanuela Bottani Ilaria Decimo |
| author_sort | Francesca Ciarpella |
| collection | DOAJ |
| description | Summary: Here we present a protocol to generate standardized cerebral organoids with hippocampal regional specification using morphogen WNT3a. We describe steps for isolating mouse embryonic (E14.5) neural stem cells from the brain subgranular zone, preparing organoids samples for immunofluorescence, calcium imaging, and metabolic profiling. This protocol can be used to generate mouse brain organoids for developmental studies, modeling disease, and drug screening. Organoids can be obtained in one month, thus providing a rapid tool for high-throughput data validation.For complete details on the use and execution of this protocol, please refer to Ciarpella et al. “Murine cerebral organoids develop network of functional neurons and hippocampal brain region identity”.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| first_indexed | 2024-03-12T23:26:56Z |
| format | Article |
| id | doaj.art-2eb4ba952a6f4341ae7fbbdf8259e910 |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-03-12T23:26:56Z |
| publishDate | 2023-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-2eb4ba952a6f4341ae7fbbdf8259e9102023-07-16T04:19:13ZengElsevierSTAR Protocols2666-16672023-09-0143102413Generation of mouse hippocampal brain organoids from primary embryonic neural stem cellsFrancesca Ciarpella0Raluca Georgiana Zamfir1Alessandra Campanelli2Giulia Pedrotti3Marzia Di Chio4Emanuela Bottani5Ilaria Decimo6Section of Pharmacology, Department of Diagnostics and Public Health, University of Verona, Verona 37134, ItalySection of Pharmacology, Department of Diagnostics and Public Health, University of Verona, Verona 37134, ItalySection of Pharmacology, Department of Diagnostics and Public Health, University of Verona, Verona 37134, ItalySection of Pharmacology, Department of Diagnostics and Public Health, University of Verona, Verona 37134, ItalySection of Pharmacology, Department of Diagnostics and Public Health, University of Verona, Verona 37134, ItalySection of Pharmacology, Department of Diagnostics and Public Health, University of Verona, Verona 37134, ItalySection of Pharmacology, Department of Diagnostics and Public Health, University of Verona, Verona 37134, Italy; Corresponding authorSummary: Here we present a protocol to generate standardized cerebral organoids with hippocampal regional specification using morphogen WNT3a. We describe steps for isolating mouse embryonic (E14.5) neural stem cells from the brain subgranular zone, preparing organoids samples for immunofluorescence, calcium imaging, and metabolic profiling. This protocol can be used to generate mouse brain organoids for developmental studies, modeling disease, and drug screening. Organoids can be obtained in one month, thus providing a rapid tool for high-throughput data validation.For complete details on the use and execution of this protocol, please refer to Ciarpella et al. “Murine cerebral organoids develop network of functional neurons and hippocampal brain region identity”.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166723003805Cell BiologyModel OrganismsNeuroscienceStem CellsCell DifferentiationOrganoids |
| spellingShingle | Francesca Ciarpella Raluca Georgiana Zamfir Alessandra Campanelli Giulia Pedrotti Marzia Di Chio Emanuela Bottani Ilaria Decimo Generation of mouse hippocampal brain organoids from primary embryonic neural stem cells STAR Protocols Cell Biology Model Organisms Neuroscience Stem Cells Cell Differentiation Organoids |
| title | Generation of mouse hippocampal brain organoids from primary embryonic neural stem cells |
| title_full | Generation of mouse hippocampal brain organoids from primary embryonic neural stem cells |
| title_fullStr | Generation of mouse hippocampal brain organoids from primary embryonic neural stem cells |
| title_full_unstemmed | Generation of mouse hippocampal brain organoids from primary embryonic neural stem cells |
| title_short | Generation of mouse hippocampal brain organoids from primary embryonic neural stem cells |
| title_sort | generation of mouse hippocampal brain organoids from primary embryonic neural stem cells |
| topic | Cell Biology Model Organisms Neuroscience Stem Cells Cell Differentiation Organoids |
| url | http://www.sciencedirect.com/science/article/pii/S2666166723003805 |
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