Generation of mouse hippocampal brain organoids from primary embryonic neural stem cells

Generation of mouse hippocampal brain organoids from primary embryonic neural stem cells

Summary: Here we present a protocol to generate standardized cerebral organoids with hippocampal regional specification using morphogen WNT3a. We describe steps for isolating mouse embryonic (E14.5) neural stem cells from the brain subgranular zone, preparing organoids samples for immunofluorescence...

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Bibliographic Details
Main Authors: Francesca Ciarpella, Raluca Georgiana Zamfir, Alessandra Campanelli, Giulia Pedrotti, Marzia Di Chio, Emanuela Bottani, Ilaria Decimo
Format: Article
Language:English
Published: Elsevier 2023-09-01
Series:STAR Protocols
Subjects:
Cell Biology
Model Organisms
Neuroscience
Stem Cells
Cell Differentiation
Organoids
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166723003805

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