Preliminary characterization of the oral microbiota of Chinese adults with and without gingivitis
<p>Abstract</p> <p>Background</p> <p>Microbial communities inhabiting human mouth are associated with oral health and disease. Previous studies have indicated the general prevalence of adult gingivitis in China to be high. The aim of this study was to characterize in de...
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| Format: | Article |
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BMC
2011-12-01
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| Series: | BMC Oral Health |
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| Online Access: | http://www.biomedcentral.com/1472-6831/11/33 |
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| author | Huang Shi Yang Fang Zeng Xiaowei Chen Jie Li Rui Wen Ting Li Chun Wei Wei Liu Jiquan Chen Lan Davis Catherine Xu Jian |
| author_facet | Huang Shi Yang Fang Zeng Xiaowei Chen Jie Li Rui Wen Ting Li Chun Wei Wei Liu Jiquan Chen Lan Davis Catherine Xu Jian |
| author_sort | Huang Shi |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Microbial communities inhabiting human mouth are associated with oral health and disease. Previous studies have indicated the general prevalence of adult gingivitis in China to be high. The aim of this study was to characterize in depth the oral microbiota of Chinese adults with or without gingivitis, by defining the microbial phylogenetic diversity and community-structure using highly paralleled pyrosequencing.</p> <p>Methods</p> <p>Six non-smoking Chinese, three with and three without gingivitis (age range 21-39 years, 4 females and 2 males) were enrolled in the present cross-sectional study. Gingival parameters of inflammation and bleeding on probing were characterized by a clinician using the Mazza Gingival Index (MGI). Plaque (sampled separately from four different oral sites) and salivary samples were obtained from each subject. Sequences and relative abundance of the bacterial 16 S rDNA PCR-amplicons were determined via pyrosequencing that produced 400 bp-long reads. The sequence data were analyzed via a computational pipeline customized for human oral microbiome analyses. Furthermore, the relative abundances of selected microbial groups were validated using quantitative PCR.</p> <p>Results</p> <p>The oral microbiomes from gingivitis and healthy subjects could be distinguished based on the distinct community structures of plaque microbiomes, but not the salivary microbiomes. Contributions of community members to community structure divergence were statistically accessed at the phylum, genus and species-like levels. Eight predominant taxa were found associated with gingivitis: TM7, <it>Leptotrichia</it>, <it>Selenomonas</it>, <it>Streptococcus</it>, <it>Veillonella</it>, <it>Prevotella</it>, <it>Lautropia</it>, and <it>Haemophilus</it>. Furthermore, 98 species-level OTUs were identified to be gingivitis-associated, which provided microbial features of gingivitis at a species resolution. Finally, for the two selected genera <it>Streptococcus </it>and <it>Fusobacterium</it>, Real-Time PCR based quantification of relative bacterial abundance validated the pyrosequencing-based results.</p> <p>Conclusions</p> <p>This methods study suggests that oral samples from this patient population of gingivitis can be characterized via plaque microbiome by pyrosequencing the 16 S rDNA genes. Further studies that characterize serial samples from subjects (longitudinal study design) with a larger population size may provide insight into the temporal and ecological features of oral microbial communities in clinically-defined states of gingivitis.</p> |
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| spelling | doaj.art-2eba662f8160437bb1e1fab51c5e2d3f2022-12-21T18:49:15ZengBMCBMC Oral Health1472-68312011-12-011113310.1186/1472-6831-11-33Preliminary characterization of the oral microbiota of Chinese adults with and without gingivitisHuang ShiYang FangZeng XiaoweiChen JieLi RuiWen TingLi ChunWei WeiLiu JiquanChen LanDavis CatherineXu Jian<p>Abstract</p> <p>Background</p> <p>Microbial communities inhabiting human mouth are associated with oral health and disease. Previous studies have indicated the general prevalence of adult gingivitis in China to be high. The aim of this study was to characterize in depth the oral microbiota of Chinese adults with or without gingivitis, by defining the microbial phylogenetic diversity and community-structure using highly paralleled pyrosequencing.</p> <p>Methods</p> <p>Six non-smoking Chinese, three with and three without gingivitis (age range 21-39 years, 4 females and 2 males) were enrolled in the present cross-sectional study. Gingival parameters of inflammation and bleeding on probing were characterized by a clinician using the Mazza Gingival Index (MGI). Plaque (sampled separately from four different oral sites) and salivary samples were obtained from each subject. Sequences and relative abundance of the bacterial 16 S rDNA PCR-amplicons were determined via pyrosequencing that produced 400 bp-long reads. The sequence data were analyzed via a computational pipeline customized for human oral microbiome analyses. Furthermore, the relative abundances of selected microbial groups were validated using quantitative PCR.</p> <p>Results</p> <p>The oral microbiomes from gingivitis and healthy subjects could be distinguished based on the distinct community structures of plaque microbiomes, but not the salivary microbiomes. Contributions of community members to community structure divergence were statistically accessed at the phylum, genus and species-like levels. Eight predominant taxa were found associated with gingivitis: TM7, <it>Leptotrichia</it>, <it>Selenomonas</it>, <it>Streptococcus</it>, <it>Veillonella</it>, <it>Prevotella</it>, <it>Lautropia</it>, and <it>Haemophilus</it>. Furthermore, 98 species-level OTUs were identified to be gingivitis-associated, which provided microbial features of gingivitis at a species resolution. Finally, for the two selected genera <it>Streptococcus </it>and <it>Fusobacterium</it>, Real-Time PCR based quantification of relative bacterial abundance validated the pyrosequencing-based results.</p> <p>Conclusions</p> <p>This methods study suggests that oral samples from this patient population of gingivitis can be characterized via plaque microbiome by pyrosequencing the 16 S rDNA genes. Further studies that characterize serial samples from subjects (longitudinal study design) with a larger population size may provide insight into the temporal and ecological features of oral microbial communities in clinically-defined states of gingivitis.</p>http://www.biomedcentral.com/1472-6831/11/33oral microbiotagingivitissalivaplaquepyrosequencing |
| spellingShingle | Huang Shi Yang Fang Zeng Xiaowei Chen Jie Li Rui Wen Ting Li Chun Wei Wei Liu Jiquan Chen Lan Davis Catherine Xu Jian Preliminary characterization of the oral microbiota of Chinese adults with and without gingivitis BMC Oral Health oral microbiota gingivitis saliva plaque pyrosequencing |
| title | Preliminary characterization of the oral microbiota of Chinese adults with and without gingivitis |
| title_full | Preliminary characterization of the oral microbiota of Chinese adults with and without gingivitis |
| title_fullStr | Preliminary characterization of the oral microbiota of Chinese adults with and without gingivitis |
| title_full_unstemmed | Preliminary characterization of the oral microbiota of Chinese adults with and without gingivitis |
| title_short | Preliminary characterization of the oral microbiota of Chinese adults with and without gingivitis |
| title_sort | preliminary characterization of the oral microbiota of chinese adults with and without gingivitis |
| topic | oral microbiota gingivitis saliva plaque pyrosequencing |
| url | http://www.biomedcentral.com/1472-6831/11/33 |
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