The Importance of Complementary PCR Analysis in Addition to Serological Testing for the Detection of Transmission Sources of <i>Brucella</i> spp. in Greek Ruminants
The early and accurate diagnosis of brucellosis, a ubiquitous zoonotic infection, is significant in preventing disease transmission. This study aimed to assess the infection rate of <i>Brucella</i> spp. in ruminants and to evaluate the agreement between a serological test and a molecular...
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MDPI AG
2022-04-01
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| Series: | Veterinary Sciences |
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| Online Access: | https://www.mdpi.com/2306-7381/9/4/193 |
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| author | Anthimia Batrinou Irini F. Strati Andreas G. Tsantes Joseph Papaparaskevas Ioannis Dimou Dimitrios Vourvidis Anna Kyrma Dionysis Antonopoulos Panagiotis Halvatsiotis Dimitra Houhoula |
| author_facet | Anthimia Batrinou Irini F. Strati Andreas G. Tsantes Joseph Papaparaskevas Ioannis Dimou Dimitrios Vourvidis Anna Kyrma Dionysis Antonopoulos Panagiotis Halvatsiotis Dimitra Houhoula |
| author_sort | Anthimia Batrinou |
| collection | DOAJ |
| description | The early and accurate diagnosis of brucellosis, a ubiquitous zoonotic infection, is significant in preventing disease transmission. This study aimed to assess the infection rate of <i>Brucella</i> spp. in ruminants and to evaluate the agreement between a serological test and a molecular method for the detection of infected cases. Blood and milk samples of 136 ruminants were analyzed using two laboratory methods: the Rose Bengal plate (RBP) test to detect <i>B. abortus</i> and <i>B. melitensis</i> antibodies and the molecular polymerase chain reaction (PCR) method for the presence of bacterial DNA. The agreement between the methods was assessed using the kappa statistic. Based on the RBP test, there were 12 (8.8%) seropositive animals (10 sheep and 2 cows), while 2 (1.4%) samples were positive on PCR analysis. The positive PCR samples were from seronegative cow samples on RBP testing. There was slight agreement (k = −0.02) between the two methods, which was not statistically significant. Our results indicate that complementary molecular methods are useful to detect the bacteria in infected animals that are seronegative due to an early stage of infection. Therefore, a combination of molecular methods and serological tests can be applied to detect brucellosis in ruminants efficiently. |
| first_indexed | 2024-03-09T12:52:40Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2306-7381 |
| language | English |
| last_indexed | 2024-03-09T12:52:40Z |
| publishDate | 2022-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Veterinary Sciences |
| spelling | doaj.art-2ebabb2645a54c0f96db5361978af29e2023-11-30T22:03:08ZengMDPI AGVeterinary Sciences2306-73812022-04-019419310.3390/vetsci9040193The Importance of Complementary PCR Analysis in Addition to Serological Testing for the Detection of Transmission Sources of <i>Brucella</i> spp. in Greek RuminantsAnthimia Batrinou0Irini F. Strati1Andreas G. Tsantes2Joseph Papaparaskevas3Ioannis Dimou4Dimitrios Vourvidis5Anna Kyrma6Dionysis Antonopoulos7Panagiotis Halvatsiotis8Dimitra Houhoula9Department of Food Science and Technology, University of West Attica, 12243 Egaleo, GreeceDepartment of Food Science and Technology, University of West Attica, 12243 Egaleo, GreeceMicrobiology Department, ‘Saint Savvas’ Oncology Hospital, 11522 Athens, GreeceDepartment of Microbiology, Medical School, National and Kapodistrian University of Athens, 15772 Athens, GreeceDepartment of Food Science and Technology, University of West Attica, 12243 Egaleo, GreeceLaboratory of Brucellosis and Bacterial Disease Serology, Department of Diagnostic Pathology, Anatomy, Histology and Microbiology Directorate of Athens Veterinary Center, Ministry of Rural Development and Food, 11522 Athens, GreeceLaboratory of Brucellosis and Bacterial Disease Serology, Department of Diagnostic Pathology, Anatomy, Histology and Microbiology Directorate of Athens Veterinary Center, Ministry of Rural Development and Food, 11522 Athens, GreeceDepartment of Food Science and Technology, University of West Attica, 12243 Egaleo, Greece2nd Propaedeutic Department of Internal Medicine, Medical School, National and Kapodistrian University of Athens, “ATTIKON” University Hospital, Chaidari 12462, GreeceDepartment of Food Science and Technology, University of West Attica, 12243 Egaleo, GreeceThe early and accurate diagnosis of brucellosis, a ubiquitous zoonotic infection, is significant in preventing disease transmission. This study aimed to assess the infection rate of <i>Brucella</i> spp. in ruminants and to evaluate the agreement between a serological test and a molecular method for the detection of infected cases. Blood and milk samples of 136 ruminants were analyzed using two laboratory methods: the Rose Bengal plate (RBP) test to detect <i>B. abortus</i> and <i>B. melitensis</i> antibodies and the molecular polymerase chain reaction (PCR) method for the presence of bacterial DNA. The agreement between the methods was assessed using the kappa statistic. Based on the RBP test, there were 12 (8.8%) seropositive animals (10 sheep and 2 cows), while 2 (1.4%) samples were positive on PCR analysis. The positive PCR samples were from seronegative cow samples on RBP testing. There was slight agreement (k = −0.02) between the two methods, which was not statistically significant. Our results indicate that complementary molecular methods are useful to detect the bacteria in infected animals that are seronegative due to an early stage of infection. Therefore, a combination of molecular methods and serological tests can be applied to detect brucellosis in ruminants efficiently.https://www.mdpi.com/2306-7381/9/4/193brucellosisruminantsPCRantibodiesDNARBPT |
| spellingShingle | Anthimia Batrinou Irini F. Strati Andreas G. Tsantes Joseph Papaparaskevas Ioannis Dimou Dimitrios Vourvidis Anna Kyrma Dionysis Antonopoulos Panagiotis Halvatsiotis Dimitra Houhoula The Importance of Complementary PCR Analysis in Addition to Serological Testing for the Detection of Transmission Sources of <i>Brucella</i> spp. in Greek Ruminants Veterinary Sciences brucellosis ruminants PCR antibodies DNA RBPT |
| title | The Importance of Complementary PCR Analysis in Addition to Serological Testing for the Detection of Transmission Sources of <i>Brucella</i> spp. in Greek Ruminants |
| title_full | The Importance of Complementary PCR Analysis in Addition to Serological Testing for the Detection of Transmission Sources of <i>Brucella</i> spp. in Greek Ruminants |
| title_fullStr | The Importance of Complementary PCR Analysis in Addition to Serological Testing for the Detection of Transmission Sources of <i>Brucella</i> spp. in Greek Ruminants |
| title_full_unstemmed | The Importance of Complementary PCR Analysis in Addition to Serological Testing for the Detection of Transmission Sources of <i>Brucella</i> spp. in Greek Ruminants |
| title_short | The Importance of Complementary PCR Analysis in Addition to Serological Testing for the Detection of Transmission Sources of <i>Brucella</i> spp. in Greek Ruminants |
| title_sort | importance of complementary pcr analysis in addition to serological testing for the detection of transmission sources of i brucella i spp in greek ruminants |
| topic | brucellosis ruminants PCR antibodies DNA RBPT |
| url | https://www.mdpi.com/2306-7381/9/4/193 |
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