Identification of Pathogenecity of Avian Influenza Virus Subtype H5N1 from Waterfowls Base on Amino Acid Sequence of Cleavage Site Hemagglutinin Protein
Identification of pathotype of Avian Influenza Virus (AIV) subtype H5N1 isolates is very important. This<br />research aimed to identify the pathotype of AIV subtype H5N1 isolated from household waterfowls in West Java<br />based on molecular markers of amino acid sequences of the Hemagg...
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| Format: | Article |
| Language: | English |
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Universitas Gadjah Mada, Yogyakarta
2015-11-01
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| Series: | Indonesian Journal of Biotechnology |
| Online Access: | http://journal.ugm.ac.id/ijbiotech/article/view/7803 |
| _version_ | 1818135894481174528 |
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| author | Susanti R. Retno D. Soejoedono Gusti Ngurah I K Mahardika Wayan T I Wibawan Maggy T Suhartono |
| author_facet | Susanti R. Retno D. Soejoedono Gusti Ngurah I K Mahardika Wayan T I Wibawan Maggy T Suhartono |
| author_sort | Susanti R. |
| collection | DOAJ |
| description | Identification of pathotype of Avian Influenza Virus (AIV) subtype H5N1 isolates is very important. This<br />research aimed to identify the pathotype of AIV subtype H5N1 isolated from household waterfowls in West Java<br />based on molecular markers of amino acid sequences of the Hemagglutinin (HA) cleavage site. Fragments of HA<br />genes of 21 isolates were amplified using RT-PCR with a primer pair that flanking the cleavage site region, and<br />sequenced with dideoxy-termination method with ABI automatic sequencer (Applied Biosystems). Multiple alignment<br />of nucleotide and their deduced amino acid sequence were analyzed using ClustalW from MEGA 3.1 program.<br />The result shows that all H5N1 isolates (21 isolates) possess polybasic cleavage sites with 2 patterns of<br />amino acid sequence, i.e QRERRRKKR (20 isolates) and QRESRRKKR (1 isolate). This finding indicates that all of<br />the viruses isolated in this research were of highly pathogenic avian influenza (HPAI) strains.<br />Keywords: cleavage site, waterfowls, HPAI |
| first_indexed | 2024-12-11T09:31:46Z |
| format | Article |
| id | doaj.art-2ebb638f493b4fbba28410f4fb1b6c51 |
| institution | Directory Open Access Journal |
| issn | 0853-8654 2089-2241 |
| language | English |
| last_indexed | 2024-12-11T09:31:46Z |
| publishDate | 2015-11-01 |
| publisher | Universitas Gadjah Mada, Yogyakarta |
| record_format | Article |
| series | Indonesian Journal of Biotechnology |
| spelling | doaj.art-2ebb638f493b4fbba28410f4fb1b6c512022-12-22T01:12:59ZengUniversitas Gadjah Mada, YogyakartaIndonesian Journal of Biotechnology0853-86542089-22412015-11-011326519Identification of Pathogenecity of Avian Influenza Virus Subtype H5N1 from Waterfowls Base on Amino Acid Sequence of Cleavage Site Hemagglutinin ProteinSusanti R.Retno D. SoejoedonoGusti Ngurah I K MahardikaWayan T I WibawanMaggy T SuhartonoIdentification of pathotype of Avian Influenza Virus (AIV) subtype H5N1 isolates is very important. This<br />research aimed to identify the pathotype of AIV subtype H5N1 isolated from household waterfowls in West Java<br />based on molecular markers of amino acid sequences of the Hemagglutinin (HA) cleavage site. Fragments of HA<br />genes of 21 isolates were amplified using RT-PCR with a primer pair that flanking the cleavage site region, and<br />sequenced with dideoxy-termination method with ABI automatic sequencer (Applied Biosystems). Multiple alignment<br />of nucleotide and their deduced amino acid sequence were analyzed using ClustalW from MEGA 3.1 program.<br />The result shows that all H5N1 isolates (21 isolates) possess polybasic cleavage sites with 2 patterns of<br />amino acid sequence, i.e QRERRRKKR (20 isolates) and QRESRRKKR (1 isolate). This finding indicates that all of<br />the viruses isolated in this research were of highly pathogenic avian influenza (HPAI) strains.<br />Keywords: cleavage site, waterfowls, HPAIhttp://journal.ugm.ac.id/ijbiotech/article/view/7803 |
| spellingShingle | Susanti R. Retno D. Soejoedono Gusti Ngurah I K Mahardika Wayan T I Wibawan Maggy T Suhartono Identification of Pathogenecity of Avian Influenza Virus Subtype H5N1 from Waterfowls Base on Amino Acid Sequence of Cleavage Site Hemagglutinin Protein Indonesian Journal of Biotechnology |
| title | Identification of Pathogenecity of Avian Influenza Virus Subtype H5N1 from Waterfowls Base on Amino Acid Sequence of Cleavage Site Hemagglutinin Protein |
| title_full | Identification of Pathogenecity of Avian Influenza Virus Subtype H5N1 from Waterfowls Base on Amino Acid Sequence of Cleavage Site Hemagglutinin Protein |
| title_fullStr | Identification of Pathogenecity of Avian Influenza Virus Subtype H5N1 from Waterfowls Base on Amino Acid Sequence of Cleavage Site Hemagglutinin Protein |
| title_full_unstemmed | Identification of Pathogenecity of Avian Influenza Virus Subtype H5N1 from Waterfowls Base on Amino Acid Sequence of Cleavage Site Hemagglutinin Protein |
| title_short | Identification of Pathogenecity of Avian Influenza Virus Subtype H5N1 from Waterfowls Base on Amino Acid Sequence of Cleavage Site Hemagglutinin Protein |
| title_sort | identification of pathogenecity of avian influenza virus subtype h5n1 from waterfowls base on amino acid sequence of cleavage site hemagglutinin protein |
| url | http://journal.ugm.ac.id/ijbiotech/article/view/7803 |
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