Decalepis hamiltonii root fraction alleviates CCl4 hepatotoxicity in a rat model
Background: Decalepis hamiltonii (D. hamiltonii) is Indian folk medicine in herbal preparations, to reduce appetite, and cures dysentery, bronchitis, uterine hemorrhage, and other ailments. Objective: The current investigation focused on the hepatoprotective effect of D. hamiltonii roots fractions a...
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Elsevier
2023-11-01
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| Series: | Journal of Ayurveda and Integrative Medicine |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0975947623001353 |
| _version_ | 1797374207951110144 |
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| author | Sonali Labhade Smita Jain Sohan Chitlange Sarvesh Paliwal Swapnil Sharma |
| author_facet | Sonali Labhade Smita Jain Sohan Chitlange Sarvesh Paliwal Swapnil Sharma |
| author_sort | Sonali Labhade |
| collection | DOAJ |
| description | Background: Decalepis hamiltonii (D. hamiltonii) is Indian folk medicine in herbal preparations, to reduce appetite, and cures dysentery, bronchitis, uterine hemorrhage, and other ailments. Objective: The current investigation focused on the hepatoprotective effect of D. hamiltonii roots fractions against liver damage. Materials and methods: The current research discussed the fraction from D. hamiltonii root extracts was used. Male Wistar rats (albino strain) were grouped into 4 distinct groups of six animals each. Group I: plain water and vehicle whereas Group II (CCl4 control): CCl4 (1 ml/kg, 20 % v/v in olive oil) over 7 days and vehicle; Over 7 days, Group III received Silymarin 100 mg/kg/day and tap water with 20 % v/v of CCl4, whereas Group IV (treatment group) received DHE 50 mg/kg/day, 100 mg/kg/day, and water. Assessment of biochemical parameters, Mitochondrial modulation, gene expression analysis, and RT-PCR, was used to estimate the protective action of DHEF in CCl4-intoxicated rats. Results: The administration of CCl4 increased levels of total bilirubin (0.63 ± 0.97 mg/dl) plasma amino transferases (110.36 ± 1.13 U/L, 86.56 ± 2.41 U/L and 1.51 ± 1.36 mg/dl respectively) which were mitigated by D. hamiltonii treatment. Activity like Lipid peroxidation and content of nitric oxide also augmented, while the antioxidant action measured by GSH (9.64 ± 0.18 U/mg protein), SOD (3.69 ± 0.22 U/mg protein), and CAT (1.47 ± 0.01 U/mg protein) was reduced. Decalepis hamiltonii root provided substantial restoration of GSH (14.92 ± 0.04 nmol/gm protein), SOD (4.20 ± 0.18 U/mg protein), and CAT (2.71 ± 0.04 U/mg protein) levels. In addition, the acute phase reactants stimulated by CCl4 administration enhanced mRNA expressions of IL-6, IL-10, TNF-a, NF-κβ, and COX-2, which were enhanced by D. hamiltonii treatment. Conclusions: In summary, DHEF protects the liver against CCl4-induced damage, possibly by mitochondrial modulation mechanism. These findings indicate that D. hamiltonii significantly moderates oxidative stress of CCl4-induced hepatotoxicity. |
| first_indexed | 2024-03-08T19:01:37Z |
| format | Article |
| id | doaj.art-2ebb6ebed3ce439982ff8a98f5a4e75d |
| institution | Directory Open Access Journal |
| issn | 0975-9476 |
| language | English |
| last_indexed | 2024-03-08T19:01:37Z |
| publishDate | 2023-11-01 |
| publisher | Elsevier |
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| series | Journal of Ayurveda and Integrative Medicine |
| spelling | doaj.art-2ebb6ebed3ce439982ff8a98f5a4e75d2023-12-28T05:14:44ZengElsevierJournal of Ayurveda and Integrative Medicine0975-94762023-11-01146100818Decalepis hamiltonii root fraction alleviates CCl4 hepatotoxicity in a rat modelSonali Labhade0Smita Jain1Sohan Chitlange2Sarvesh Paliwal3Swapnil Sharma4Banasthali Vidyapith, Rajasthan, India; Dr. D.Y.Patil Institute of Pharmaceutical Sciences and Research, Pimpri, Pune, India; Corresponding author. Banasthali Vidyapith, Rajasthan, India.Banasthali Vidyapith, Rajasthan, IndiaDr. D.Y.Patil Institute of Pharmaceutical Sciences and Research, Pimpri, Pune, IndiaBanasthali Vidyapith, Rajasthan, IndiaBanasthali Vidyapith, Rajasthan, IndiaBackground: Decalepis hamiltonii (D. hamiltonii) is Indian folk medicine in herbal preparations, to reduce appetite, and cures dysentery, bronchitis, uterine hemorrhage, and other ailments. Objective: The current investigation focused on the hepatoprotective effect of D. hamiltonii roots fractions against liver damage. Materials and methods: The current research discussed the fraction from D. hamiltonii root extracts was used. Male Wistar rats (albino strain) were grouped into 4 distinct groups of six animals each. Group I: plain water and vehicle whereas Group II (CCl4 control): CCl4 (1 ml/kg, 20 % v/v in olive oil) over 7 days and vehicle; Over 7 days, Group III received Silymarin 100 mg/kg/day and tap water with 20 % v/v of CCl4, whereas Group IV (treatment group) received DHE 50 mg/kg/day, 100 mg/kg/day, and water. Assessment of biochemical parameters, Mitochondrial modulation, gene expression analysis, and RT-PCR, was used to estimate the protective action of DHEF in CCl4-intoxicated rats. Results: The administration of CCl4 increased levels of total bilirubin (0.63 ± 0.97 mg/dl) plasma amino transferases (110.36 ± 1.13 U/L, 86.56 ± 2.41 U/L and 1.51 ± 1.36 mg/dl respectively) which were mitigated by D. hamiltonii treatment. Activity like Lipid peroxidation and content of nitric oxide also augmented, while the antioxidant action measured by GSH (9.64 ± 0.18 U/mg protein), SOD (3.69 ± 0.22 U/mg protein), and CAT (1.47 ± 0.01 U/mg protein) was reduced. Decalepis hamiltonii root provided substantial restoration of GSH (14.92 ± 0.04 nmol/gm protein), SOD (4.20 ± 0.18 U/mg protein), and CAT (2.71 ± 0.04 U/mg protein) levels. In addition, the acute phase reactants stimulated by CCl4 administration enhanced mRNA expressions of IL-6, IL-10, TNF-a, NF-κβ, and COX-2, which were enhanced by D. hamiltonii treatment. Conclusions: In summary, DHEF protects the liver against CCl4-induced damage, possibly by mitochondrial modulation mechanism. These findings indicate that D. hamiltonii significantly moderates oxidative stress of CCl4-induced hepatotoxicity.http://www.sciencedirect.com/science/article/pii/S0975947623001353AntioxidantsDecalepis hamiltoniiCarbon tetrachlorideN- butanol |
| spellingShingle | Sonali Labhade Smita Jain Sohan Chitlange Sarvesh Paliwal Swapnil Sharma Decalepis hamiltonii root fraction alleviates CCl4 hepatotoxicity in a rat model Journal of Ayurveda and Integrative Medicine Antioxidants Decalepis hamiltonii Carbon tetrachloride N- butanol |
| title | Decalepis hamiltonii root fraction alleviates CCl4 hepatotoxicity in a rat model |
| title_full | Decalepis hamiltonii root fraction alleviates CCl4 hepatotoxicity in a rat model |
| title_fullStr | Decalepis hamiltonii root fraction alleviates CCl4 hepatotoxicity in a rat model |
| title_full_unstemmed | Decalepis hamiltonii root fraction alleviates CCl4 hepatotoxicity in a rat model |
| title_short | Decalepis hamiltonii root fraction alleviates CCl4 hepatotoxicity in a rat model |
| title_sort | decalepis hamiltonii root fraction alleviates ccl4 hepatotoxicity in a rat model |
| topic | Antioxidants Decalepis hamiltonii Carbon tetrachloride N- butanol |
| url | http://www.sciencedirect.com/science/article/pii/S0975947623001353 |
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