Porcine reproductive and respiratory syndrome virus: web-based interactive tools to support surveillance and control initiatives
Abstract Background Control of porcine reproductive and respiratory syndrome (PRRS) represents a tremendous challenge. The trend is now toward managing the disease collectively. In Quebec, area and regional control and elimination (ARC&E) initiatives started in 2011; diagnostic testing, includin...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2019-03-01
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| Series: | Porcine Health Management |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s40813-019-0117-x |
| _version_ | 1818460134291013632 |
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| author | Marie-Ève Lambert Pascal Audet Benjamin Delisle Julie Arsenault Sylvie D’Allaire |
| author_facet | Marie-Ève Lambert Pascal Audet Benjamin Delisle Julie Arsenault Sylvie D’Allaire |
| author_sort | Marie-Ève Lambert |
| collection | DOAJ |
| description | Abstract Background Control of porcine reproductive and respiratory syndrome (PRRS) represents a tremendous challenge. The trend is now toward managing the disease collectively. In Quebec, area and regional control and elimination (ARC&E) initiatives started in 2011; diagnostic testing, including ORF5 sequencing, and sharing of information among stakeholders are largely promoted. At the provincial level, a data-sharing agreement was signed by Quebec swine practitioners allowing PRRS virus (PRRSV) sequences to be transferred to a database maintained by the Laboratoire d’épidémiologie et de médecine porcine (LEMP-DB). Several interactive tools were developed and are available to veterinarians to allow comparison of PRRSV ORF5 sequences within ARC&E projects or provincially while managing confidentiality issues. Results Between January 1st 2010 and December 31st 2018, 4346 PRRSV ORF5 sequences were gathered into the LEMP-DB, involving 1254 sites and 43 practicing veterinarians. Approximately 34% of the submissions were from ARC&E projects. Using a novel web-based sequence comparison tool, each veterinarian has access to information on his/her client sequences and can compare each sequence with 1) commercial vaccine strains, 2) historical samples from the same site, and 3) all sequences submitted to the database over the last 4 years. Newly introduced PRRSV into breeding herds can be monitored using a new sequence comparison tool based on comparison of sequences at the provincial level. Each month, graphs providing the number of introductions per month and the yearly cumulative are updated. Between August 1st 2014 and December 31st 2018, 233 introductions were detected on 180 different breeding sites. Following a data-sharing agreement, veterinarians involved in ARC&E projects have access to an interactive mapping tool to locate pig sites, compare sequence similarity between participating sites and visualize the results on the map. Conclusions The structure developed in Quebec to collect, analyse and share sequencing data was efficient to provide useful information to the swine industry at both provincial and regional levels while dealing with confidentiality issues. |
| first_indexed | 2024-12-14T23:25:25Z |
| format | Article |
| id | doaj.art-2ebe1dc34087493987cbaef82cd010a0 |
| institution | Directory Open Access Journal |
| issn | 2055-5660 |
| language | English |
| last_indexed | 2024-12-14T23:25:25Z |
| publishDate | 2019-03-01 |
| publisher | BMC |
| record_format | Article |
| series | Porcine Health Management |
| spelling | doaj.art-2ebe1dc34087493987cbaef82cd010a02022-12-21T22:43:49ZengBMCPorcine Health Management2055-56602019-03-015111010.1186/s40813-019-0117-xPorcine reproductive and respiratory syndrome virus: web-based interactive tools to support surveillance and control initiativesMarie-Ève Lambert0Pascal Audet1Benjamin Delisle2Julie Arsenault3Sylvie D’Allaire4Laboratoire d’épidémiologie et de médecine porcine (LEMP), Faculty of Veterinary Medicine, Université de MontréalLaboratoire d’épidémiologie et de médecine porcine (LEMP), Faculty of Veterinary Medicine, Université de MontréalLaboratoire d’épidémiologie et de médecine porcine (LEMP), Faculty of Veterinary Medicine, Université de MontréalLaboratoire d’épidémiologie et de médecine porcine (LEMP), Faculty of Veterinary Medicine, Université de MontréalLaboratoire d’épidémiologie et de médecine porcine (LEMP), Faculty of Veterinary Medicine, Université de MontréalAbstract Background Control of porcine reproductive and respiratory syndrome (PRRS) represents a tremendous challenge. The trend is now toward managing the disease collectively. In Quebec, area and regional control and elimination (ARC&E) initiatives started in 2011; diagnostic testing, including ORF5 sequencing, and sharing of information among stakeholders are largely promoted. At the provincial level, a data-sharing agreement was signed by Quebec swine practitioners allowing PRRS virus (PRRSV) sequences to be transferred to a database maintained by the Laboratoire d’épidémiologie et de médecine porcine (LEMP-DB). Several interactive tools were developed and are available to veterinarians to allow comparison of PRRSV ORF5 sequences within ARC&E projects or provincially while managing confidentiality issues. Results Between January 1st 2010 and December 31st 2018, 4346 PRRSV ORF5 sequences were gathered into the LEMP-DB, involving 1254 sites and 43 practicing veterinarians. Approximately 34% of the submissions were from ARC&E projects. Using a novel web-based sequence comparison tool, each veterinarian has access to information on his/her client sequences and can compare each sequence with 1) commercial vaccine strains, 2) historical samples from the same site, and 3) all sequences submitted to the database over the last 4 years. Newly introduced PRRSV into breeding herds can be monitored using a new sequence comparison tool based on comparison of sequences at the provincial level. Each month, graphs providing the number of introductions per month and the yearly cumulative are updated. Between August 1st 2014 and December 31st 2018, 233 introductions were detected on 180 different breeding sites. Following a data-sharing agreement, veterinarians involved in ARC&E projects have access to an interactive mapping tool to locate pig sites, compare sequence similarity between participating sites and visualize the results on the map. Conclusions The structure developed in Quebec to collect, analyse and share sequencing data was efficient to provide useful information to the swine industry at both provincial and regional levels while dealing with confidentiality issues.http://link.springer.com/article/10.1186/s40813-019-0117-xPRRSSequenceORF5SimilarityEpidemiologyControl |
| spellingShingle | Marie-Ève Lambert Pascal Audet Benjamin Delisle Julie Arsenault Sylvie D’Allaire Porcine reproductive and respiratory syndrome virus: web-based interactive tools to support surveillance and control initiatives Porcine Health Management PRRS Sequence ORF5 Similarity Epidemiology Control |
| title | Porcine reproductive and respiratory syndrome virus: web-based interactive tools to support surveillance and control initiatives |
| title_full | Porcine reproductive and respiratory syndrome virus: web-based interactive tools to support surveillance and control initiatives |
| title_fullStr | Porcine reproductive and respiratory syndrome virus: web-based interactive tools to support surveillance and control initiatives |
| title_full_unstemmed | Porcine reproductive and respiratory syndrome virus: web-based interactive tools to support surveillance and control initiatives |
| title_short | Porcine reproductive and respiratory syndrome virus: web-based interactive tools to support surveillance and control initiatives |
| title_sort | porcine reproductive and respiratory syndrome virus web based interactive tools to support surveillance and control initiatives |
| topic | PRRS Sequence ORF5 Similarity Epidemiology Control |
| url | http://link.springer.com/article/10.1186/s40813-019-0117-x |
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