Staphylococcus aureus skin colonization is mediated by SasG lectin variation

Summary: Staphylococcus aureus causes the majority of skin and soft tissue infections, but this pathogen only transiently colonizes healthy skin. However, this transient skin exposure enables S. aureus to transition to infection. The initial adhesion of S. aureus to skin corneocytes is mediated by s...

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Main Authors: Krista B. Mills, Joseph J. Maciag, Can Wang, John A. Crawford, Timothy J. Enroth, Klara C. Keim, Yves F. Dufrêne, D. Ashley Robinson, Paul D. Fey, Andrew B. Herr, Alexander R. Horswill
Format: Article
Language:English
Published: Elsevier 2024-04-01
Series:Cell Reports
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2211124724003504
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author Krista B. Mills
Joseph J. Maciag
Can Wang
John A. Crawford
Timothy J. Enroth
Klara C. Keim
Yves F. Dufrêne
D. Ashley Robinson
Paul D. Fey
Andrew B. Herr
Alexander R. Horswill
author_facet Krista B. Mills
Joseph J. Maciag
Can Wang
John A. Crawford
Timothy J. Enroth
Klara C. Keim
Yves F. Dufrêne
D. Ashley Robinson
Paul D. Fey
Andrew B. Herr
Alexander R. Horswill
author_sort Krista B. Mills
collection DOAJ
description Summary: Staphylococcus aureus causes the majority of skin and soft tissue infections, but this pathogen only transiently colonizes healthy skin. However, this transient skin exposure enables S. aureus to transition to infection. The initial adhesion of S. aureus to skin corneocytes is mediated by surface protein G (SasG). Here, phylogenetic analyses reveal the presence of two major divergent SasG alleles in S. aureus: SasG-I and SasG-II. Structural analyses of SasG-II identify a nonaromatic arginine in the binding pocket of the lectin subdomain that mediates adhesion to corneocytes. Atomic force microscopy and corneocyte adhesion assays indicate that SasG-II can bind to a broader variety of ligands than SasG-I. Glycosidase treatment results in different binding profiles between SasG-I and SasG-II on skin cells. In addition, SasG-mediated adhesion is recapitulated using differentiated N/TERT keratinocytes. Our findings indicate that SasG-II has evolved to adhere to multiple ligands, conferring a distinct advantage to S. aureus during skin colonization.
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spelling doaj.art-2ebfbddde1c54212aebe1e6dc23bb64a2024-04-03T04:26:31ZengElsevierCell Reports2211-12472024-04-01434114022Staphylococcus aureus skin colonization is mediated by SasG lectin variationKrista B. Mills0Joseph J. Maciag1Can Wang2John A. Crawford3Timothy J. Enroth4Klara C. Keim5Yves F. Dufrêne6D. Ashley Robinson7Paul D. Fey8Andrew B. Herr9Alexander R. Horswill10Department of Immunology and Microbiology, University of Colorado Anschutz Medical Campus, Aurora, CO, USADivision of Immunobiology, Cincinnati Children’s Hospital Medical Center, Cincinnati, OH, USALouvain Institute of Biomolecular Science and Technology, UCLouvain, Louvain-la-Neuve, BelgiumDepartment of Cell and Molecular Biology, University of Mississippi Medical Center, Jackson, MS, USADepartment of Immunology and Microbiology, University of Colorado Anschutz Medical Campus, Aurora, CO, USADepartment of Immunology and Microbiology, University of Colorado Anschutz Medical Campus, Aurora, CO, USALouvain Institute of Biomolecular Science and Technology, UCLouvain, Louvain-la-Neuve, BelgiumDepartment of Cell and Molecular Biology, University of Mississippi Medical Center, Jackson, MS, USA; Center for Immunology and Microbial Research, University of Mississippi Medical Center, Jackson, MS, USADepartment of Pathology and Microbiology, University of Nebraska Medical Center, Omaha, NE, USADivision of Immunobiology, Cincinnati Children’s Hospital Medical Center, Cincinnati, OH, USA; Division of Infectious Diseases, Cincinnati Children’s Hospital Medical Center, Cincinnati, OH, USA; Department of Pediatrics, University of Cincinnati College of Medicine, Cincinnati, OH, USADepartment of Immunology and Microbiology, University of Colorado Anschutz Medical Campus, Aurora, CO, USA; Department of Veterans Affairs, VA Eastern Colorado Healthcare System, Aurora, CO, USA; Corresponding authorSummary: Staphylococcus aureus causes the majority of skin and soft tissue infections, but this pathogen only transiently colonizes healthy skin. However, this transient skin exposure enables S. aureus to transition to infection. The initial adhesion of S. aureus to skin corneocytes is mediated by surface protein G (SasG). Here, phylogenetic analyses reveal the presence of two major divergent SasG alleles in S. aureus: SasG-I and SasG-II. Structural analyses of SasG-II identify a nonaromatic arginine in the binding pocket of the lectin subdomain that mediates adhesion to corneocytes. Atomic force microscopy and corneocyte adhesion assays indicate that SasG-II can bind to a broader variety of ligands than SasG-I. Glycosidase treatment results in different binding profiles between SasG-I and SasG-II on skin cells. In addition, SasG-mediated adhesion is recapitulated using differentiated N/TERT keratinocytes. Our findings indicate that SasG-II has evolved to adhere to multiple ligands, conferring a distinct advantage to S. aureus during skin colonization.http://www.sciencedirect.com/science/article/pii/S2211124724003504CP: Microbiology
spellingShingle Krista B. Mills
Joseph J. Maciag
Can Wang
John A. Crawford
Timothy J. Enroth
Klara C. Keim
Yves F. Dufrêne
D. Ashley Robinson
Paul D. Fey
Andrew B. Herr
Alexander R. Horswill
Staphylococcus aureus skin colonization is mediated by SasG lectin variation
Cell Reports
CP: Microbiology
title Staphylococcus aureus skin colonization is mediated by SasG lectin variation
title_full Staphylococcus aureus skin colonization is mediated by SasG lectin variation
title_fullStr Staphylococcus aureus skin colonization is mediated by SasG lectin variation
title_full_unstemmed Staphylococcus aureus skin colonization is mediated by SasG lectin variation
title_short Staphylococcus aureus skin colonization is mediated by SasG lectin variation
title_sort staphylococcus aureus skin colonization is mediated by sasg lectin variation
topic CP: Microbiology
url http://www.sciencedirect.com/science/article/pii/S2211124724003504
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