Recent progress in the development of DNA-based biosensors integrated with hybridization chain reaction or catalytic hairpin assembly
As isothermal, enzyme-free signal amplification strategies, hybridization chain reaction (HCR) and catalytic hairpin assembly (CHA) possess the advantages such as high amplification efficiency, excellent biocompatibility, mild reactions, and easy operation. Therefore, they have been widely applied i...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-02-01
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| Series: | Frontiers in Chemistry |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fchem.2023.1134863/full |
| _version_ | 1797904965714313216 |
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| author | Liuting Mo Wanqi He Ziyi Li Danlian Liang Runhong Qin Mingxiu Mo Chan Yang Weiying Lin |
| author_facet | Liuting Mo Wanqi He Ziyi Li Danlian Liang Runhong Qin Mingxiu Mo Chan Yang Weiying Lin |
| author_sort | Liuting Mo |
| collection | DOAJ |
| description | As isothermal, enzyme-free signal amplification strategies, hybridization chain reaction (HCR) and catalytic hairpin assembly (CHA) possess the advantages such as high amplification efficiency, excellent biocompatibility, mild reactions, and easy operation. Therefore, they have been widely applied in DNA-based biosensors for detecting small molecules, nucleic acids, and proteins. In this review, we summarize the recent progress of DNA-based sensors employing typical and advanced HCR and CHA strategies, including branched HCR or CHA, localized HCR or CHA, and cascaded reactions. In addition, the bottlenecks of implementing HCR and CHA in biosensing applications are discussed, such as high background signals, lower amplification efficiency than enzyme-assisted techniques, slow kinetics, poor stability, and internalization of DNA probes in cellular applications. |
| first_indexed | 2024-04-10T09:57:24Z |
| format | Article |
| id | doaj.art-2ec2d279ce0347919c38de227538a223 |
| institution | Directory Open Access Journal |
| issn | 2296-2646 |
| language | English |
| last_indexed | 2024-04-10T09:57:24Z |
| publishDate | 2023-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Chemistry |
| spelling | doaj.art-2ec2d279ce0347919c38de227538a2232023-02-16T11:57:43ZengFrontiers Media S.A.Frontiers in Chemistry2296-26462023-02-011110.3389/fchem.2023.11348631134863Recent progress in the development of DNA-based biosensors integrated with hybridization chain reaction or catalytic hairpin assemblyLiuting MoWanqi HeZiyi LiDanlian LiangRunhong QinMingxiu MoChan YangWeiying LinAs isothermal, enzyme-free signal amplification strategies, hybridization chain reaction (HCR) and catalytic hairpin assembly (CHA) possess the advantages such as high amplification efficiency, excellent biocompatibility, mild reactions, and easy operation. Therefore, they have been widely applied in DNA-based biosensors for detecting small molecules, nucleic acids, and proteins. In this review, we summarize the recent progress of DNA-based sensors employing typical and advanced HCR and CHA strategies, including branched HCR or CHA, localized HCR or CHA, and cascaded reactions. In addition, the bottlenecks of implementing HCR and CHA in biosensing applications are discussed, such as high background signals, lower amplification efficiency than enzyme-assisted techniques, slow kinetics, poor stability, and internalization of DNA probes in cellular applications.https://www.frontiersin.org/articles/10.3389/fchem.2023.1134863/fullhybridization chain reactioncatalytic hairpin assemblybiosensordisease diagnosiscell imaging |
| spellingShingle | Liuting Mo Wanqi He Ziyi Li Danlian Liang Runhong Qin Mingxiu Mo Chan Yang Weiying Lin Recent progress in the development of DNA-based biosensors integrated with hybridization chain reaction or catalytic hairpin assembly Frontiers in Chemistry hybridization chain reaction catalytic hairpin assembly biosensor disease diagnosis cell imaging |
| title | Recent progress in the development of DNA-based biosensors integrated with hybridization chain reaction or catalytic hairpin assembly |
| title_full | Recent progress in the development of DNA-based biosensors integrated with hybridization chain reaction or catalytic hairpin assembly |
| title_fullStr | Recent progress in the development of DNA-based biosensors integrated with hybridization chain reaction or catalytic hairpin assembly |
| title_full_unstemmed | Recent progress in the development of DNA-based biosensors integrated with hybridization chain reaction or catalytic hairpin assembly |
| title_short | Recent progress in the development of DNA-based biosensors integrated with hybridization chain reaction or catalytic hairpin assembly |
| title_sort | recent progress in the development of dna based biosensors integrated with hybridization chain reaction or catalytic hairpin assembly |
| topic | hybridization chain reaction catalytic hairpin assembly biosensor disease diagnosis cell imaging |
| url | https://www.frontiersin.org/articles/10.3389/fchem.2023.1134863/full |
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