In-Vitro Evaluation of Hydroalcoholic Extract of Allium Ampeloprasum on Rat Ileum Contractions
Background: Ampeloprasum is traditionally used for gastrointestinal disorders. In this experimental study, the effect of ethanolic extract of Allium ampeloprasum was investigated on the rat ileum contractions. The mechanisms were also evaluated. Methods: In this experimental study, 48 male Wistar r...
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Isfahan University of Medical Sciences
2014-03-01
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| Series: | مجله دانشکده پزشکی اصفهان |
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| Online Access: | http://jims.mui.ac.ir/index.php/jims/article/view/3016 |
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| author | Masoumeh Sedighi-Hafshejani Mosayyeb Noori-Ahmadabadi Hamid Nasri Molok Hadi Mahmoud Rafieian-Kopaei |
| author_facet | Masoumeh Sedighi-Hafshejani Mosayyeb Noori-Ahmadabadi Hamid Nasri Molok Hadi Mahmoud Rafieian-Kopaei |
| author_sort | Masoumeh Sedighi-Hafshejani |
| collection | DOAJ |
| description | Background: Ampeloprasum is traditionally used for gastrointestinal disorders. In this experimental study, the effect of ethanolic extract of Allium ampeloprasum was investigated on the rat ileum contractions. The mechanisms were also evaluated.
Methods: In this experimental study, 48 male Wistar rats were randomly designated into six equal groups, including control and cumulative concentrations of Allium ampeloprasum, propranolol, naloxone, L-name, and CaCl2. In the day of the experiment, the ileum was dissected and its contractions were recorded in Tyrode solution in an organ bath. To evaluate the mechanisms involved, propranolol, naloxone, or L-name was incubated and the percent of changes in contractions were recorded.
Findings: The extract of Allium ampeloprasum dose-dependently reduced the contractions induced by KCl (60 mM) (P < 0.0001); propranolol reversed this effect (P < 0.0010), but L-name and naloxone were not effective. CaCl2 increased the contractions (P < 0.0010) and the extract reversed this effect.
Conclusion: Allium ampeloprasum leaves can affect the ileum contractions through beta adrenoreceptors and voltage-dependent calcium channels and might be used in gastrointestinal disorders. |
| first_indexed | 2024-03-12T08:57:33Z |
| format | Article |
| id | doaj.art-2ec69d3c3de541e1a720f4e036bd8664 |
| institution | Directory Open Access Journal |
| issn | 1027-7595 1735-854X |
| language | fas |
| last_indexed | 2024-03-12T08:57:33Z |
| publishDate | 2014-03-01 |
| publisher | Isfahan University of Medical Sciences |
| record_format | Article |
| series | مجله دانشکده پزشکی اصفهان |
| spelling | doaj.art-2ec69d3c3de541e1a720f4e036bd86642023-09-02T15:53:21ZfasIsfahan University of Medical Sciencesمجله دانشکده پزشکی اصفهان1027-75951735-854X2014-03-0131268223822491501In-Vitro Evaluation of Hydroalcoholic Extract of Allium Ampeloprasum on Rat Ileum ContractionsMasoumeh Sedighi-Hafshejani0Mosayyeb Noori-Ahmadabadi1Hamid Nasri2Molok Hadi3Mahmoud Rafieian-Kopaei4Medical Plants Research Center, Shahrekord University of Medical Sciences, Shahrekord, IranStudent of Medicine, Medical Plants Research Center, Shahrekord University of Medical Sciences, Shahrekord, IranProfessor, Department of Nephrology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, IranDepartment of Statistics, School of Health, Shahrekord University of Medical Sciences, Shahrekord, IranProfessor, Medical Plants Research Center, Shahrekord University of Medical Sciences, Shahrekord, IranBackground: Ampeloprasum is traditionally used for gastrointestinal disorders. In this experimental study, the effect of ethanolic extract of Allium ampeloprasum was investigated on the rat ileum contractions. The mechanisms were also evaluated. Methods: In this experimental study, 48 male Wistar rats were randomly designated into six equal groups, including control and cumulative concentrations of Allium ampeloprasum, propranolol, naloxone, L-name, and CaCl2. In the day of the experiment, the ileum was dissected and its contractions were recorded in Tyrode solution in an organ bath. To evaluate the mechanisms involved, propranolol, naloxone, or L-name was incubated and the percent of changes in contractions were recorded. Findings: The extract of Allium ampeloprasum dose-dependently reduced the contractions induced by KCl (60 mM) (P < 0.0001); propranolol reversed this effect (P < 0.0010), but L-name and naloxone were not effective. CaCl2 increased the contractions (P < 0.0010) and the extract reversed this effect. Conclusion: Allium ampeloprasum leaves can affect the ileum contractions through beta adrenoreceptors and voltage-dependent calcium channels and might be used in gastrointestinal disorders.http://jims.mui.ac.ir/index.php/jims/article/view/3016Allium ampeloprasumIleumBeta-blockers |
| spellingShingle | Masoumeh Sedighi-Hafshejani Mosayyeb Noori-Ahmadabadi Hamid Nasri Molok Hadi Mahmoud Rafieian-Kopaei In-Vitro Evaluation of Hydroalcoholic Extract of Allium Ampeloprasum on Rat Ileum Contractions مجله دانشکده پزشکی اصفهان Allium ampeloprasum Ileum Beta-blockers |
| title | In-Vitro Evaluation of Hydroalcoholic Extract of Allium Ampeloprasum on Rat Ileum Contractions |
| title_full | In-Vitro Evaluation of Hydroalcoholic Extract of Allium Ampeloprasum on Rat Ileum Contractions |
| title_fullStr | In-Vitro Evaluation of Hydroalcoholic Extract of Allium Ampeloprasum on Rat Ileum Contractions |
| title_full_unstemmed | In-Vitro Evaluation of Hydroalcoholic Extract of Allium Ampeloprasum on Rat Ileum Contractions |
| title_short | In-Vitro Evaluation of Hydroalcoholic Extract of Allium Ampeloprasum on Rat Ileum Contractions |
| title_sort | in vitro evaluation of hydroalcoholic extract of allium ampeloprasum on rat ileum contractions |
| topic | Allium ampeloprasum Ileum Beta-blockers |
| url | http://jims.mui.ac.ir/index.php/jims/article/view/3016 |
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