Fluorescence Imaging of 3D Cell Models with Subcellular Resolution
Over the past years, research has made impressive breakthroughs towards the development and implementation of 3D cell models for a wide range of applications, such as drug development and testing, organogenesis, cancer biology, and personalized medicine. Opposed to 2D cell monolayer culture systems,...
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| Format: | Artikel |
| Sprache: | English |
| Veröffentlicht: |
Bio-protocol LLC
2022-07-01
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| Schriftenreihe: | Bio-Protocol |
| Online Zugang: | https://bio-protocol.org/en/bpdetail?id=4469&type=0 |
| _version_ | 1831869550117781504 |
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| author | Indra Van Zundert Nina Maenhoudt Silke De Vriendt Hugo Vankelecom Beatrice Fortuni Susana Rocha |
| author_facet | Indra Van Zundert Nina Maenhoudt Silke De Vriendt Hugo Vankelecom Beatrice Fortuni Susana Rocha |
| author_sort | Indra Van Zundert |
| collection | DOAJ |
| description | Over the past years, research has made impressive breakthroughs towards the development and implementation of 3D cell models for a wide range of applications, such as drug development and testing, organogenesis, cancer biology, and personalized medicine. Opposed to 2D cell monolayer culture systems, advanced 3D cell models better represent the in vivo physiology. However, for these models to deliver scientific insights, appropriate investigation techniques are required. Despite the potential of fluorescence microscopy to visualize these models with high spatial resolution, sample preparation and imaging assays are not straightforward. Here, we provide different protocols of sample preparation for fluorescence imaging, for both matrix-embedded and matrix-free models (e.g., organoids and spheroids, respectively). Additionally, we provide detailed guidelines for imaging 3D cell models via confocal multi-photon fluorescence microscopy. We show that using these protocols, images of 3D cell culture systems can be obtained with sub-cellular resolution.Graphical abstract: |
| first_indexed | 2025-03-21T19:12:07Z |
| format | Article |
| id | doaj.art-2ec70714b51b4c3cb835573e7fa6d7c6 |
| institution | Directory Open Access Journal |
| issn | 2331-8325 |
| language | English |
| last_indexed | 2025-03-21T19:12:07Z |
| publishDate | 2022-07-01 |
| publisher | Bio-protocol LLC |
| record_format | Article |
| series | Bio-Protocol |
| spelling | doaj.art-2ec70714b51b4c3cb835573e7fa6d7c62024-06-04T09:40:51ZengBio-protocol LLCBio-Protocol2331-83252022-07-01121410.21769/BioProtoc.4469Fluorescence Imaging of 3D Cell Models with Subcellular ResolutionIndra Van Zundert0Nina Maenhoudt1Silke De Vriendt2Hugo Vankelecom3Beatrice Fortuni4Susana Rocha5Molecular Imaging and Photonics, Department of Chemistry, KU Leuven, Celestijnenlaan 200F, 3001 Heverlee, BelgiumLaboratory of Tissue Plasticity in Health and Disease, Stem Cell and Developmental Biology Cluster, Department of Development and Regeneration, KU Leuven, Leuven, BelgiumLaboratory of Tissue Plasticity in Health and Disease, Stem Cell and Developmental Biology Cluster, Department of Development and Regeneration, KU Leuven, Leuven, BelgiumLaboratory of Tissue Plasticity in Health and Disease, Stem Cell and Developmental Biology Cluster, Department of Development and Regeneration, KU Leuven, Leuven, BelgiumMolecular Imaging and Photonics, Department of Chemistry, KU Leuven, Celestijnenlaan 200F, 3001 Heverlee, BelgiumMolecular Imaging and Photonics, Department of Chemistry, KU Leuven, Celestijnenlaan 200F, 3001 Heverlee, BelgiumOver the past years, research has made impressive breakthroughs towards the development and implementation of 3D cell models for a wide range of applications, such as drug development and testing, organogenesis, cancer biology, and personalized medicine. Opposed to 2D cell monolayer culture systems, advanced 3D cell models better represent the in vivo physiology. However, for these models to deliver scientific insights, appropriate investigation techniques are required. Despite the potential of fluorescence microscopy to visualize these models with high spatial resolution, sample preparation and imaging assays are not straightforward. Here, we provide different protocols of sample preparation for fluorescence imaging, for both matrix-embedded and matrix-free models (e.g., organoids and spheroids, respectively). Additionally, we provide detailed guidelines for imaging 3D cell models via confocal multi-photon fluorescence microscopy. We show that using these protocols, images of 3D cell culture systems can be obtained with sub-cellular resolution.Graphical abstract: https://bio-protocol.org/en/bpdetail?id=4469&type=0 |
| spellingShingle | Indra Van Zundert Nina Maenhoudt Silke De Vriendt Hugo Vankelecom Beatrice Fortuni Susana Rocha Fluorescence Imaging of 3D Cell Models with Subcellular Resolution Bio-Protocol |
| title | Fluorescence Imaging of 3D Cell Models with Subcellular Resolution |
| title_full | Fluorescence Imaging of 3D Cell Models with Subcellular Resolution |
| title_fullStr | Fluorescence Imaging of 3D Cell Models with Subcellular Resolution |
| title_full_unstemmed | Fluorescence Imaging of 3D Cell Models with Subcellular Resolution |
| title_short | Fluorescence Imaging of 3D Cell Models with Subcellular Resolution |
| title_sort | fluorescence imaging of 3d cell models with subcellular resolution |
| url | https://bio-protocol.org/en/bpdetail?id=4469&type=0 |
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