Usefulness of circulating microRNAs miR-146a and miR-16-5p as prognostic biomarkers in community-acquired pneumonia.
<h4>Introduction</h4>Patients with community-acquired pneumonia (CAP) undergo a dysregulated host response that is related to mortality. MicroRNAs (miRNAs) participate in this response, but their expression pattern and their role as biomarkers in CAP have not been fully characterized.<...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2020-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0240926 |
| _version_ | 1818965414201262080 |
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| author | José María Galván-Román Ángel Lancho-Sánchez Sergio Luquero-Bueno Lorena Vega-Piris Jose Curbelo Marcos Manzaneque-Pradales Manuel Gómez Hortensia de la Fuente Mara Ortega-Gómez Javier Aspa |
| author_facet | José María Galván-Román Ángel Lancho-Sánchez Sergio Luquero-Bueno Lorena Vega-Piris Jose Curbelo Marcos Manzaneque-Pradales Manuel Gómez Hortensia de la Fuente Mara Ortega-Gómez Javier Aspa |
| author_sort | José María Galván-Román |
| collection | DOAJ |
| description | <h4>Introduction</h4>Patients with community-acquired pneumonia (CAP) undergo a dysregulated host response that is related to mortality. MicroRNAs (miRNAs) participate in this response, but their expression pattern and their role as biomarkers in CAP have not been fully characterized.<h4>Methods</h4>A prospective observational study was performed in a cohort of 153 consecutive patients admitted to hospital with CAP. Clinical and analytical variables were collected, and the main outcome variable was 30-day mortality. Small RNA was purified from plasma of these patients obtained on the first day of admission, and miRNA expression was analyzed by RT-PCR. Univariate and multivariate analyses were carried out through the construction of a logistic regression model. The proposed model was compared with established prognostic clinical scales using ROC curve analysis.<h4>Results</h4>The mean age of the patients included was 74.7 years [SD 15.9]. Their mean PSI was 100.9 [SD 34.6] and the mean modified Charlson index was 2.9 [SD 3.0]. Both miR-146a and miR-16-5p showed statistically significant association with 30-day mortality after admission due to CAP (1.10 vs. 0.23 and 51.74 vs. 35.23, respectively), and this association remained for miR-16-5p in the multivariate analysis adjusted for age, gender and history of bronchoaspiration (OR 0.95, p = 0.021). The area-under-the-curve (AUC) of our adjusted multivariate model (AUC = 0.954 95%CI [0.91-0.99]), was better than those of prognostic scales such as PSI (AUC = 0.799 [0.69-0.91]) and CURB-65 (AUC = 0.722 [0.58-0.86]).<h4>Conclusions</h4>High levels of miR-146a-5p and miR-16-5p upon admission due to CAP are associated with lower mortality at 30 days of follow-up. Both miRNAs could be used as biomarkers of good prognosis in subjects hospitalized with CAP. |
| first_indexed | 2024-12-20T13:16:37Z |
| format | Article |
| id | doaj.art-2ec9376c856a41ca932babac429ba4da |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-20T13:16:37Z |
| publishDate | 2020-01-01 |
| publisher | Public Library of Science (PLoS) |
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| series | PLoS ONE |
| spelling | doaj.art-2ec9376c856a41ca932babac429ba4da2022-12-21T19:39:31ZengPublic Library of Science (PLoS)PLoS ONE1932-62032020-01-011510e024092610.1371/journal.pone.0240926Usefulness of circulating microRNAs miR-146a and miR-16-5p as prognostic biomarkers in community-acquired pneumonia.José María Galván-RománÁngel Lancho-SánchezSergio Luquero-BuenoLorena Vega-PirisJose CurbeloMarcos Manzaneque-PradalesManuel GómezHortensia de la FuenteMara Ortega-GómezJavier Aspa<h4>Introduction</h4>Patients with community-acquired pneumonia (CAP) undergo a dysregulated host response that is related to mortality. MicroRNAs (miRNAs) participate in this response, but their expression pattern and their role as biomarkers in CAP have not been fully characterized.<h4>Methods</h4>A prospective observational study was performed in a cohort of 153 consecutive patients admitted to hospital with CAP. Clinical and analytical variables were collected, and the main outcome variable was 30-day mortality. Small RNA was purified from plasma of these patients obtained on the first day of admission, and miRNA expression was analyzed by RT-PCR. Univariate and multivariate analyses were carried out through the construction of a logistic regression model. The proposed model was compared with established prognostic clinical scales using ROC curve analysis.<h4>Results</h4>The mean age of the patients included was 74.7 years [SD 15.9]. Their mean PSI was 100.9 [SD 34.6] and the mean modified Charlson index was 2.9 [SD 3.0]. Both miR-146a and miR-16-5p showed statistically significant association with 30-day mortality after admission due to CAP (1.10 vs. 0.23 and 51.74 vs. 35.23, respectively), and this association remained for miR-16-5p in the multivariate analysis adjusted for age, gender and history of bronchoaspiration (OR 0.95, p = 0.021). The area-under-the-curve (AUC) of our adjusted multivariate model (AUC = 0.954 95%CI [0.91-0.99]), was better than those of prognostic scales such as PSI (AUC = 0.799 [0.69-0.91]) and CURB-65 (AUC = 0.722 [0.58-0.86]).<h4>Conclusions</h4>High levels of miR-146a-5p and miR-16-5p upon admission due to CAP are associated with lower mortality at 30 days of follow-up. Both miRNAs could be used as biomarkers of good prognosis in subjects hospitalized with CAP.https://doi.org/10.1371/journal.pone.0240926 |
| spellingShingle | José María Galván-Román Ángel Lancho-Sánchez Sergio Luquero-Bueno Lorena Vega-Piris Jose Curbelo Marcos Manzaneque-Pradales Manuel Gómez Hortensia de la Fuente Mara Ortega-Gómez Javier Aspa Usefulness of circulating microRNAs miR-146a and miR-16-5p as prognostic biomarkers in community-acquired pneumonia. PLoS ONE |
| title | Usefulness of circulating microRNAs miR-146a and miR-16-5p as prognostic biomarkers in community-acquired pneumonia. |
| title_full | Usefulness of circulating microRNAs miR-146a and miR-16-5p as prognostic biomarkers in community-acquired pneumonia. |
| title_fullStr | Usefulness of circulating microRNAs miR-146a and miR-16-5p as prognostic biomarkers in community-acquired pneumonia. |
| title_full_unstemmed | Usefulness of circulating microRNAs miR-146a and miR-16-5p as prognostic biomarkers in community-acquired pneumonia. |
| title_short | Usefulness of circulating microRNAs miR-146a and miR-16-5p as prognostic biomarkers in community-acquired pneumonia. |
| title_sort | usefulness of circulating micrornas mir 146a and mir 16 5p as prognostic biomarkers in community acquired pneumonia |
| url | https://doi.org/10.1371/journal.pone.0240926 |
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