A banana transcriptional repressor MaAP2a participates in fruit starch degradation during postharvest ripening
Fruit postharvest ripening is a crucial course for many fruits with significant conversion of biosubstance, which forms an intricate regulatory network. Ethylene facilitates the ripening process in banana with a remarkable change of fruit starch, but the mechanism adjusting the expression of starch...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-11-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2022.1036719/full |
| _version_ | 1798018515206144000 |
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| author | Yunyi Xiao Ying Li Lejun Ouyang Aiguo Yin Bo Xu Ling Zhang Jianye Chen Jinfeng Liu |
| author_facet | Yunyi Xiao Ying Li Lejun Ouyang Aiguo Yin Bo Xu Ling Zhang Jianye Chen Jinfeng Liu |
| author_sort | Yunyi Xiao |
| collection | DOAJ |
| description | Fruit postharvest ripening is a crucial course for many fruits with significant conversion of biosubstance, which forms an intricate regulatory network. Ethylene facilitates the ripening process in banana with a remarkable change of fruit starch, but the mechanism adjusting the expression of starch degradation-related enzyme genes is incompletely discovered. Here, we describe a banana APETALA2 transcription factor (MaAP2a) identified as a transcriptional repressor with its powerful transcriptional inhibitory activity. The transcriptional level of MaAP2a gradually decreased with the transition of banana fruit ripening, suggesting a passive role of MaAP2a in banana fruit ripening. Moreover, MaAP2a is a classic nucleoprotein and encompasses transcriptional repressor domain (EAR, LxLxLx). More specifically, protein–DNA interaction assays found that MaAP2a repressed the expression of 15 starch degradation-related genes comprising MaGWD1, MaPWD1, MaSEX4, MaLSF1, MaBAM1-MaBAM3, MaAMY2B/2C/3A/3C, MaMEX1/2, and MapGlcT2-1/2-2 via binding to the GCC-box or AT-rich motif of their promoters. Overall, these results reveal an original MaAP2a-mediated negative regulatory network involved in banana postharvest starch breakdown, which advances our cognition on banana fruit ripening and offers additional reference values for banana varietal improvement. |
| first_indexed | 2024-04-11T16:25:24Z |
| format | Article |
| id | doaj.art-2ec9c689fba44e5a96963efef8d145a4 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-11T16:25:24Z |
| publishDate | 2022-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-2ec9c689fba44e5a96963efef8d145a42022-12-22T04:14:12ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2022-11-011310.3389/fpls.2022.10367191036719A banana transcriptional repressor MaAP2a participates in fruit starch degradation during postharvest ripeningYunyi Xiao0Ying Li1Lejun Ouyang2Aiguo Yin3Bo Xu4Ling Zhang5Jianye Chen6Jinfeng Liu7College of Biological and Food Engineering, Guangdong University of Petrochemical Technology, Maoming, ChinaCollege of Biological and Food Engineering, Guangdong University of Petrochemical Technology, Maoming, ChinaCollege of Biological and Food Engineering, Guangdong University of Petrochemical Technology, Maoming, ChinaCollege of Biological and Food Engineering, Guangdong University of Petrochemical Technology, Maoming, ChinaCollege of Biological and Food Engineering, Guangdong University of Petrochemical Technology, Maoming, ChinaCollege of Biological and Food Engineering, Guangdong University of Petrochemical Technology, Maoming, ChinaCollege of Horticultural Science, South China Agricultural University, Guangzhou, ChinaCollege of Biological and Food Engineering, Guangdong University of Petrochemical Technology, Maoming, ChinaFruit postharvest ripening is a crucial course for many fruits with significant conversion of biosubstance, which forms an intricate regulatory network. Ethylene facilitates the ripening process in banana with a remarkable change of fruit starch, but the mechanism adjusting the expression of starch degradation-related enzyme genes is incompletely discovered. Here, we describe a banana APETALA2 transcription factor (MaAP2a) identified as a transcriptional repressor with its powerful transcriptional inhibitory activity. The transcriptional level of MaAP2a gradually decreased with the transition of banana fruit ripening, suggesting a passive role of MaAP2a in banana fruit ripening. Moreover, MaAP2a is a classic nucleoprotein and encompasses transcriptional repressor domain (EAR, LxLxLx). More specifically, protein–DNA interaction assays found that MaAP2a repressed the expression of 15 starch degradation-related genes comprising MaGWD1, MaPWD1, MaSEX4, MaLSF1, MaBAM1-MaBAM3, MaAMY2B/2C/3A/3C, MaMEX1/2, and MapGlcT2-1/2-2 via binding to the GCC-box or AT-rich motif of their promoters. Overall, these results reveal an original MaAP2a-mediated negative regulatory network involved in banana postharvest starch breakdown, which advances our cognition on banana fruit ripening and offers additional reference values for banana varietal improvement.https://www.frontiersin.org/articles/10.3389/fpls.2022.1036719/fullbanana fruitpostharvest ripeningAPETALA2starch degradationtranscriptional repressor |
| spellingShingle | Yunyi Xiao Ying Li Lejun Ouyang Aiguo Yin Bo Xu Ling Zhang Jianye Chen Jinfeng Liu A banana transcriptional repressor MaAP2a participates in fruit starch degradation during postharvest ripening Frontiers in Plant Science banana fruit postharvest ripening APETALA2 starch degradation transcriptional repressor |
| title | A banana transcriptional repressor MaAP2a participates in fruit starch degradation during postharvest ripening |
| title_full | A banana transcriptional repressor MaAP2a participates in fruit starch degradation during postharvest ripening |
| title_fullStr | A banana transcriptional repressor MaAP2a participates in fruit starch degradation during postharvest ripening |
| title_full_unstemmed | A banana transcriptional repressor MaAP2a participates in fruit starch degradation during postharvest ripening |
| title_short | A banana transcriptional repressor MaAP2a participates in fruit starch degradation during postharvest ripening |
| title_sort | banana transcriptional repressor maap2a participates in fruit starch degradation during postharvest ripening |
| topic | banana fruit postharvest ripening APETALA2 starch degradation transcriptional repressor |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2022.1036719/full |
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