Proliferation and differentiation of stem cells in contact with eluate from fibrin-rich plasma membrane
ABSTRACT Objective: To evaluate the ability of the eluate from fibrin-rich plasma (FRP) membrane to induce proliferation and differentiation of isolated human adipose-derived stem cells (ASCs) into chondrocytes. Method: FRP membranes were obtained by centrifugation of peripheral blood from two h...
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Sociedade Brasileira de Ortopedia e Traumatologia
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| Series: | Revista Brasileira de Ortopedia |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-36162018000100045&lng=en&tlng=en |
| _version_ | 1797331331255894016 |
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| author | Fernanda Gimenez de Souza Beatriz Luci Fernandes Carmen Lucia Kuniyoshi Rebelatto Alessandra Melo de Aguiar Letícia Fracaro Paulo Roberto Slud Brofman |
| author_facet | Fernanda Gimenez de Souza Beatriz Luci Fernandes Carmen Lucia Kuniyoshi Rebelatto Alessandra Melo de Aguiar Letícia Fracaro Paulo Roberto Slud Brofman |
| author_sort | Fernanda Gimenez de Souza |
| collection | DOAJ |
| description | ABSTRACT Objective: To evaluate the ability of the eluate from fibrin-rich plasma (FRP) membrane to induce proliferation and differentiation of isolated human adipose-derived stem cells (ASCs) into chondrocytes. Method: FRP membranes were obtained by centrifugation of peripheral blood from two healthy donors, cut, and maintained in culture plate wells for 48 h to prepare the fibrin eluate. The SCATh were isolated from adipose tissue by collagenase digestion solution, and expanded in vitro. Cells were expanded and treated with DMEM-F12 culture, a commercial media for chondrogenic differentiation, and eluate from FRP membrane for three days, and labeled with BrdU for quantitative assessment of cell proliferation using the High-Content Operetta® imaging system. For the chondrogenic differentiation assay, the SCATh were grown in micromass for 21 days and stained with toluidine blue and aggrecan for qualitative evaluation by light microscopy. The statistical analysis was performed using ANOVA and Tukey's test. Results: There was a greater proliferation of cells treated with the eluate from FRP membrane compared to the other two treatments, where the ANOVA test showed significance (p < 0.001). The differentiation into chondrocytes was visualized by the presence of mucopolysaccharide in the matrix of the cells marked in blue toluidine and aggrecan. Conclusions: Treatment with eluate from FRP membrane stimulated cell proliferation and induced differentiation of the stem cells into chondrocytes, suggesting a potential application of FRP membranes in hyaline cartilage regeneration therapies. |
| first_indexed | 2024-03-08T07:32:56Z |
| format | Article |
| id | doaj.art-2ecff349c6a145a38f042cb6eefb08cb |
| institution | Directory Open Access Journal |
| issn | 1982-4378 |
| language | English |
| last_indexed | 2024-03-08T07:32:56Z |
| publisher | Sociedade Brasileira de Ortopedia e Traumatologia |
| record_format | Article |
| series | Revista Brasileira de Ortopedia |
| spelling | doaj.art-2ecff349c6a145a38f042cb6eefb08cb2024-02-02T19:45:18ZengSociedade Brasileira de Ortopedia e TraumatologiaRevista Brasileira de Ortopedia1982-4378531455210.1016/j.rboe.2017.12.004S0102-36162018000100045Proliferation and differentiation of stem cells in contact with eluate from fibrin-rich plasma membraneFernanda Gimenez de SouzaBeatriz Luci FernandesCarmen Lucia Kuniyoshi RebelattoAlessandra Melo de AguiarLetícia FracaroPaulo Roberto Slud BrofmanABSTRACT Objective: To evaluate the ability of the eluate from fibrin-rich plasma (FRP) membrane to induce proliferation and differentiation of isolated human adipose-derived stem cells (ASCs) into chondrocytes. Method: FRP membranes were obtained by centrifugation of peripheral blood from two healthy donors, cut, and maintained in culture plate wells for 48 h to prepare the fibrin eluate. The SCATh were isolated from adipose tissue by collagenase digestion solution, and expanded in vitro. Cells were expanded and treated with DMEM-F12 culture, a commercial media for chondrogenic differentiation, and eluate from FRP membrane for three days, and labeled with BrdU for quantitative assessment of cell proliferation using the High-Content Operetta® imaging system. For the chondrogenic differentiation assay, the SCATh were grown in micromass for 21 days and stained with toluidine blue and aggrecan for qualitative evaluation by light microscopy. The statistical analysis was performed using ANOVA and Tukey's test. Results: There was a greater proliferation of cells treated with the eluate from FRP membrane compared to the other two treatments, where the ANOVA test showed significance (p < 0.001). The differentiation into chondrocytes was visualized by the presence of mucopolysaccharide in the matrix of the cells marked in blue toluidine and aggrecan. Conclusions: Treatment with eluate from FRP membrane stimulated cell proliferation and induced differentiation of the stem cells into chondrocytes, suggesting a potential application of FRP membranes in hyaline cartilage regeneration therapies.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-36162018000100045&lng=en&tlng=enPlatelet-rich plasmaMembranesCartilageRegeneration |
| spellingShingle | Fernanda Gimenez de Souza Beatriz Luci Fernandes Carmen Lucia Kuniyoshi Rebelatto Alessandra Melo de Aguiar Letícia Fracaro Paulo Roberto Slud Brofman Proliferation and differentiation of stem cells in contact with eluate from fibrin-rich plasma membrane Revista Brasileira de Ortopedia Platelet-rich plasma Membranes Cartilage Regeneration |
| title | Proliferation and differentiation of stem cells in contact with eluate from fibrin-rich plasma membrane |
| title_full | Proliferation and differentiation of stem cells in contact with eluate from fibrin-rich plasma membrane |
| title_fullStr | Proliferation and differentiation of stem cells in contact with eluate from fibrin-rich plasma membrane |
| title_full_unstemmed | Proliferation and differentiation of stem cells in contact with eluate from fibrin-rich plasma membrane |
| title_short | Proliferation and differentiation of stem cells in contact with eluate from fibrin-rich plasma membrane |
| title_sort | proliferation and differentiation of stem cells in contact with eluate from fibrin rich plasma membrane |
| topic | Platelet-rich plasma Membranes Cartilage Regeneration |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-36162018000100045&lng=en&tlng=en |
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