Novel Rifampicin and Indocyanine Green Co-Loaded Perfluorocarbon Nanodroplets Provide Effective <i>In Vivo</i> Photo–Chemo–Probiotic Antimicrobility against Pathogen of Acne Vulgaris <i>Cutibacterium acnes</i>
Acne vulgaris is one of the most prevalent dermatological diseases among adolescents and is often associated with overgrowth of <i>Cutibacterium acnes</i> (<i>C. acnes</i>) in the pilosebaceous units. In this study, we aimed to develop novel rifampicin (RIF) and indocyanine g...
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MDPI AG
2020-06-01
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| Series: | Nanomaterials |
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| Online Access: | https://www.mdpi.com/2079-4991/10/6/1095 |
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| author | Kuang-Hung Hsiao Chun-Ming Huang Yu-Hsiang Lee |
| author_facet | Kuang-Hung Hsiao Chun-Ming Huang Yu-Hsiang Lee |
| author_sort | Kuang-Hung Hsiao |
| collection | DOAJ |
| description | Acne vulgaris is one of the most prevalent dermatological diseases among adolescents and is often associated with overgrowth of <i>Cutibacterium acnes</i> (<i>C. acnes</i>) in the pilosebaceous units. In this study, we aimed to develop novel rifampicin (RIF) and indocyanine green (ICG) co-loaded perfluorocarbon nanodroplets named RIPNDs which can simultaneously provide photo-, chemo-, and probiotic-antimicrobility, and explore their efficacy in treatment of <i>C. acnes</i> <i>in vitro</i> and <i>in vivo</i>. The RIPNDs were first characterized as being spherical in shape, with a size of 238.6 ± 7.51 nm and surface charge of −22.3 ± 3.5 mV. Then, the optimal dosages of <i>Staphylococcus epidermidis</i>–produced fermentation product medium (FPM) and RIPND were determined as 25% (<i>v</i>/<i>v</i>) and [RIF]/[ICG] = 3.8/20 μM, respectively, based on the analyses of inhibition zone and cytotoxicity <i>in vitro</i>. Through the <i>in vivo</i> study using <i>C. acnes</i>–inoculated mice, our data showed that the group treated with FPM followed by RIPNDs + near infrared (NIR) irradiation obtained the least granulocytes/macrophage-inflammatory protein 2 expression level in the epidermis, and showed a significantly lower microbial colony population compared to the groups treated with equal amount of RIF, FPM, RIPNDs, and/or combination of the above ± NIR. These results indicated that the RIPND-mediated photo–chemo–probiotic therapeutics was indeed able to rapidly suppress inflammatory response of the skin and provide a robust antibacterial effect against <i>C. acnes</i> with limited use of antibiotics. Taken altogether, we anticipate that the RIPND is highly potential for use in the clinical treatment of acne vulgaris. |
| first_indexed | 2024-03-10T19:26:31Z |
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| institution | Directory Open Access Journal |
| issn | 2079-4991 |
| language | English |
| last_indexed | 2024-03-10T19:26:31Z |
| publishDate | 2020-06-01 |
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| series | Nanomaterials |
| spelling | doaj.art-2ed0966effea41d18205d3a573c0ea152023-11-20T02:28:34ZengMDPI AGNanomaterials2079-49912020-06-01106109510.3390/nano10061095Novel Rifampicin and Indocyanine Green Co-Loaded Perfluorocarbon Nanodroplets Provide Effective <i>In Vivo</i> Photo–Chemo–Probiotic Antimicrobility against Pathogen of Acne Vulgaris <i>Cutibacterium acnes</i>Kuang-Hung Hsiao0Chun-Ming Huang1Yu-Hsiang Lee2Department of Biomedical Sciences and Engineering, National Central University. No. 300, Jhongda Rd., Taoyuan City 32001, TaiwanDepartment of Biomedical Sciences and Engineering, National Central University. No. 300, Jhongda Rd., Taoyuan City 32001, TaiwanDepartment of Biomedical Sciences and Engineering, National Central University. No. 300, Jhongda Rd., Taoyuan City 32001, TaiwanAcne vulgaris is one of the most prevalent dermatological diseases among adolescents and is often associated with overgrowth of <i>Cutibacterium acnes</i> (<i>C. acnes</i>) in the pilosebaceous units. In this study, we aimed to develop novel rifampicin (RIF) and indocyanine green (ICG) co-loaded perfluorocarbon nanodroplets named RIPNDs which can simultaneously provide photo-, chemo-, and probiotic-antimicrobility, and explore their efficacy in treatment of <i>C. acnes</i> <i>in vitro</i> and <i>in vivo</i>. The RIPNDs were first characterized as being spherical in shape, with a size of 238.6 ± 7.51 nm and surface charge of −22.3 ± 3.5 mV. Then, the optimal dosages of <i>Staphylococcus epidermidis</i>–produced fermentation product medium (FPM) and RIPND were determined as 25% (<i>v</i>/<i>v</i>) and [RIF]/[ICG] = 3.8/20 μM, respectively, based on the analyses of inhibition zone and cytotoxicity <i>in vitro</i>. Through the <i>in vivo</i> study using <i>C. acnes</i>–inoculated mice, our data showed that the group treated with FPM followed by RIPNDs + near infrared (NIR) irradiation obtained the least granulocytes/macrophage-inflammatory protein 2 expression level in the epidermis, and showed a significantly lower microbial colony population compared to the groups treated with equal amount of RIF, FPM, RIPNDs, and/or combination of the above ± NIR. These results indicated that the RIPND-mediated photo–chemo–probiotic therapeutics was indeed able to rapidly suppress inflammatory response of the skin and provide a robust antibacterial effect against <i>C. acnes</i> with limited use of antibiotics. Taken altogether, we anticipate that the RIPND is highly potential for use in the clinical treatment of acne vulgaris.https://www.mdpi.com/2079-4991/10/6/1095acne vulgarisperfluorocarbonnanoemulsionprobioticsantibioticsphototherapy |
| spellingShingle | Kuang-Hung Hsiao Chun-Ming Huang Yu-Hsiang Lee Novel Rifampicin and Indocyanine Green Co-Loaded Perfluorocarbon Nanodroplets Provide Effective <i>In Vivo</i> Photo–Chemo–Probiotic Antimicrobility against Pathogen of Acne Vulgaris <i>Cutibacterium acnes</i> Nanomaterials acne vulgaris perfluorocarbon nanoemulsion probiotics antibiotics phototherapy |
| title | Novel Rifampicin and Indocyanine Green Co-Loaded Perfluorocarbon Nanodroplets Provide Effective <i>In Vivo</i> Photo–Chemo–Probiotic Antimicrobility against Pathogen of Acne Vulgaris <i>Cutibacterium acnes</i> |
| title_full | Novel Rifampicin and Indocyanine Green Co-Loaded Perfluorocarbon Nanodroplets Provide Effective <i>In Vivo</i> Photo–Chemo–Probiotic Antimicrobility against Pathogen of Acne Vulgaris <i>Cutibacterium acnes</i> |
| title_fullStr | Novel Rifampicin and Indocyanine Green Co-Loaded Perfluorocarbon Nanodroplets Provide Effective <i>In Vivo</i> Photo–Chemo–Probiotic Antimicrobility against Pathogen of Acne Vulgaris <i>Cutibacterium acnes</i> |
| title_full_unstemmed | Novel Rifampicin and Indocyanine Green Co-Loaded Perfluorocarbon Nanodroplets Provide Effective <i>In Vivo</i> Photo–Chemo–Probiotic Antimicrobility against Pathogen of Acne Vulgaris <i>Cutibacterium acnes</i> |
| title_short | Novel Rifampicin and Indocyanine Green Co-Loaded Perfluorocarbon Nanodroplets Provide Effective <i>In Vivo</i> Photo–Chemo–Probiotic Antimicrobility against Pathogen of Acne Vulgaris <i>Cutibacterium acnes</i> |
| title_sort | novel rifampicin and indocyanine green co loaded perfluorocarbon nanodroplets provide effective i in vivo i photo chemo probiotic antimicrobility against pathogen of acne vulgaris i cutibacterium acnes i |
| topic | acne vulgaris perfluorocarbon nanoemulsion probiotics antibiotics phototherapy |
| url | https://www.mdpi.com/2079-4991/10/6/1095 |
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