Selection and Test of L-histidine Decarboxylase Enzyme Activity of Six Isolates of Histamine Forming Bacteria
Six isolates of histamine forming bacteria were screened to see the degree of ability in producing histamine on modified Niven's medium. The result showed that the six bacteria were able to produce histamine by giving a pinkish color on the medium, which could be used as a preliminary identific...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Universitas Indonesia
2007-11-01
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| Series: | Makara Seri Sains |
| Subjects: | |
| Online Access: | http://journal.ui.ac.id/science/article/viewFile/292/288 |
| _version_ | 1828432831800410112 |
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| author | Romauli Aya Sophia Endang Sri Heruwati Wibowo Mangunwardoyo |
| author_facet | Romauli Aya Sophia Endang Sri Heruwati Wibowo Mangunwardoyo |
| author_sort | Romauli Aya Sophia |
| collection | DOAJ |
| description | Six isolates of histamine forming bacteria were screened to see the degree of ability in producing histamine on modified Niven's medium. The result showed that the six bacteria were able to produce histamine by giving a pinkish color on the medium, which could be used as a preliminary identification of histamine-forming bacteria (HFB). The isolates were grown in liquid modified Niven medium to measure the production of histamine. The histamine produced were determined by Hardy and Smith method. The result showed that all of the isolates produced high level of histamine (92.35 - 305.49 mg/100 ml of the medium). From all of them, Enterobacter spp. produced the highest level of histamine (305.49 mg/100 ml). A synthetic medium was used to measure the growth pattern and optimum time required by Enterobacter spp and Morganella morganii (as control bacteria) to produce the L-histidine decarboxylase enzyme (HDC) which is responsible for histamine production. The result showed that for both bacteria, the optimum enzim production was 8 hours after incubation. |
| first_indexed | 2024-12-10T18:26:33Z |
| format | Article |
| id | doaj.art-2ed37b4c611241e3aa317050cf28a60e |
| institution | Directory Open Access Journal |
| issn | 1693-6671 |
| language | English |
| last_indexed | 2024-12-10T18:26:33Z |
| publishDate | 2007-11-01 |
| publisher | Universitas Indonesia |
| record_format | Article |
| series | Makara Seri Sains |
| spelling | doaj.art-2ed37b4c611241e3aa317050cf28a60e2022-12-22T01:38:04ZengUniversitas IndonesiaMakara Seri Sains1693-66712007-11-01112104109Selection and Test of L-histidine Decarboxylase Enzyme Activity of Six Isolates of Histamine Forming BacteriaRomauli Aya SophiaEndang Sri HeruwatiWibowo MangunwardoyoSix isolates of histamine forming bacteria were screened to see the degree of ability in producing histamine on modified Niven's medium. The result showed that the six bacteria were able to produce histamine by giving a pinkish color on the medium, which could be used as a preliminary identification of histamine-forming bacteria (HFB). The isolates were grown in liquid modified Niven medium to measure the production of histamine. The histamine produced were determined by Hardy and Smith method. The result showed that all of the isolates produced high level of histamine (92.35 - 305.49 mg/100 ml of the medium). From all of them, Enterobacter spp. produced the highest level of histamine (305.49 mg/100 ml). A synthetic medium was used to measure the growth pattern and optimum time required by Enterobacter spp and Morganella morganii (as control bacteria) to produce the L-histidine decarboxylase enzyme (HDC) which is responsible for histamine production. The result showed that for both bacteria, the optimum enzim production was 8 hours after incubation.http://journal.ui.ac.id/science/article/viewFile/292/288histamine forming bacteriaL-histidine decarboxylaseNiven's modified medium |
| spellingShingle | Romauli Aya Sophia Endang Sri Heruwati Wibowo Mangunwardoyo Selection and Test of L-histidine Decarboxylase Enzyme Activity of Six Isolates of Histamine Forming Bacteria Makara Seri Sains histamine forming bacteria L-histidine decarboxylase Niven's modified medium |
| title | Selection and Test of L-histidine Decarboxylase Enzyme Activity of Six Isolates of Histamine Forming Bacteria |
| title_full | Selection and Test of L-histidine Decarboxylase Enzyme Activity of Six Isolates of Histamine Forming Bacteria |
| title_fullStr | Selection and Test of L-histidine Decarboxylase Enzyme Activity of Six Isolates of Histamine Forming Bacteria |
| title_full_unstemmed | Selection and Test of L-histidine Decarboxylase Enzyme Activity of Six Isolates of Histamine Forming Bacteria |
| title_short | Selection and Test of L-histidine Decarboxylase Enzyme Activity of Six Isolates of Histamine Forming Bacteria |
| title_sort | selection and test of l histidine decarboxylase enzyme activity of six isolates of histamine forming bacteria |
| topic | histamine forming bacteria L-histidine decarboxylase Niven's modified medium |
| url | http://journal.ui.ac.id/science/article/viewFile/292/288 |
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