Identification of cassava germplasms resistant to two-spotted spider mite in China: From greenhouse large-scale screening to field validation
IntroductionUtilization of resistant germplasm is considered as an effective, economical and eco-friendly strategy for cassava pest management. Tetranychus urticae, known as the two-spotted spider mite (TSSM), is a devastating pest in Asian cassava planting countries as well as in China. However, th...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-12-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2022.1054909/full |
| _version_ | 1811310338642018304 |
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| author | Xiao Liang Xiao Liang Qing Chen Qing Chen Ying Liu Ying Liu Chunling Wu Chunling Wu Kaimian Li Mufeng Wu Mufeng Wu Xiaowen Yao Xiaowen Yao Yang Qiao Yang Qiao Yao Zhang Yao Zhang Yue Geng Yue Geng |
| author_facet | Xiao Liang Xiao Liang Qing Chen Qing Chen Ying Liu Ying Liu Chunling Wu Chunling Wu Kaimian Li Mufeng Wu Mufeng Wu Xiaowen Yao Xiaowen Yao Yang Qiao Yang Qiao Yao Zhang Yao Zhang Yue Geng Yue Geng |
| author_sort | Xiao Liang |
| collection | DOAJ |
| description | IntroductionUtilization of resistant germplasm is considered as an effective, economical and eco-friendly strategy for cassava pest management. Tetranychus urticae, known as the two-spotted spider mite (TSSM), is a devastating pest in Asian cassava planting countries as well as in China. However, the resistant levels of abundant cassava germplasms to TSSM remains largely unknown.MethodsTo fill this knowledge gap, we conducted screening of 202 cassava germplasm for resistance to TSSM in China based on the classification of mite damage phenotype, under both greenhouse and field conditions.ResultsThe three rounds of large-scale greenhouse experiments had identified two highly resistant (HR) varieties (C1115 and MIANDIAN), five resistant (R) varieties (SC5, SC9, SC15, COLUMBIA-4D and LIMIN) and five highly susceptible (HS) varieties (KU50, BREAD, SC205, TMS60444 and BRA900), besides, these ‘HR’ and ‘R’ varieties would significantly repress the normal development and reproduction of TSSM. In addition, the 12 cassava varieties selected from the greenhouse screening were further subjected to consecutive five years of field validation at Danzhou, Wuming and Baoshan. The seven resistant varieties not only exhibited stable TSSM-resistance performance across the three field environments, but also possessed the same resistant levels as the greenhouse identification, while the resistant varieties SC5 was an exception, which was identified as moderate resistant in Baoshan, indicating the variety-environment interaction may affect its resistance. Furthermore, regional yield estimation suggested that the higher the resistance level was, the better capacity in reducing the yield losses.DiscussionThis study demonstrated that the TSSM-resistant varieties could be considered as ideal materials in mite control or in future breeding programme of mite-resistant cassava plant. |
| first_indexed | 2024-04-13T09:57:17Z |
| format | Article |
| id | doaj.art-2ed504e8d60a45d9a764a47d04d974ba |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-13T09:57:17Z |
| publishDate | 2022-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-2ed504e8d60a45d9a764a47d04d974ba2022-12-22T02:51:19ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2022-12-011310.3389/fpls.2022.10549091054909Identification of cassava germplasms resistant to two-spotted spider mite in China: From greenhouse large-scale screening to field validationXiao Liang0Xiao Liang1Qing Chen2Qing Chen3Ying Liu4Ying Liu5Chunling Wu6Chunling Wu7Kaimian Li8Mufeng Wu9Mufeng Wu10Xiaowen Yao11Xiaowen Yao12Yang Qiao13Yang Qiao14Yao Zhang15Yao Zhang16Yue Geng17Yue Geng18Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences, Key Laboratory of Integrated Pest Management on Tropical Crops, Ministry of Agriculture and Rural Affairs, Haikou, Hainan, ChinaSanya Research Academy, Chinese Academy of Tropical Agriculture Science, Hainan Key Laboratory for Biosafety Monitoring and Molecular Breeding in Off-Season Reproduction Regions, Sanya, Hainan, ChinaEnvironment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences, Key Laboratory of Integrated Pest Management on Tropical Crops, Ministry of Agriculture and Rural Affairs, Haikou, Hainan, ChinaSanya Research Academy, Chinese Academy of Tropical Agriculture Science, Hainan Key Laboratory for Biosafety Monitoring and Molecular Breeding in Off-Season Reproduction Regions, Sanya, Hainan, ChinaEnvironment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences, Key Laboratory of Integrated Pest Management on Tropical Crops, Ministry of Agriculture and Rural Affairs, Haikou, Hainan, ChinaSanya Research Academy, Chinese Academy of Tropical Agriculture Science, Hainan Key Laboratory for Biosafety Monitoring and Molecular Breeding in Off-Season Reproduction Regions, Sanya, Hainan, ChinaEnvironment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences, Key Laboratory of Integrated Pest Management on Tropical Crops, Ministry of Agriculture and Rural Affairs, Haikou, Hainan, ChinaSanya Research Academy, Chinese Academy of Tropical Agriculture Science, Hainan Key Laboratory for Biosafety Monitoring and Molecular Breeding in Off-Season Reproduction Regions, Sanya, Hainan, ChinaTropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agriculture Sciences, Haikou, ChinaEnvironment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences, Key Laboratory of Integrated Pest Management on Tropical Crops, Ministry of Agriculture and Rural Affairs, Haikou, Hainan, ChinaSanya Research Academy, Chinese Academy of Tropical Agriculture Science, Hainan Key Laboratory for Biosafety Monitoring and Molecular Breeding in Off-Season Reproduction Regions, Sanya, Hainan, ChinaEnvironment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences, Key Laboratory of Integrated Pest Management on Tropical Crops, Ministry of Agriculture and Rural Affairs, Haikou, Hainan, ChinaSanya Research Academy, Chinese Academy of Tropical Agriculture Science, Hainan Key Laboratory for Biosafety Monitoring and Molecular Breeding in Off-Season Reproduction Regions, Sanya, Hainan, ChinaEnvironment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences, Key Laboratory of Integrated Pest Management on Tropical Crops, Ministry of Agriculture and Rural Affairs, Haikou, Hainan, ChinaSanya Research Academy, Chinese Academy of Tropical Agriculture Science, Hainan Key Laboratory for Biosafety Monitoring and Molecular Breeding in Off-Season Reproduction Regions, Sanya, Hainan, ChinaEnvironment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences, Key Laboratory of Integrated Pest Management on Tropical Crops, Ministry of Agriculture and Rural Affairs, Haikou, Hainan, ChinaSanya Research Academy, Chinese Academy of Tropical Agriculture Science, Hainan Key Laboratory for Biosafety Monitoring and Molecular Breeding in Off-Season Reproduction Regions, Sanya, Hainan, ChinaEnvironment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences, Key Laboratory of Integrated Pest Management on Tropical Crops, Ministry of Agriculture and Rural Affairs, Haikou, Hainan, ChinaSanya Research Academy, Chinese Academy of Tropical Agriculture Science, Hainan Key Laboratory for Biosafety Monitoring and Molecular Breeding in Off-Season Reproduction Regions, Sanya, Hainan, ChinaIntroductionUtilization of resistant germplasm is considered as an effective, economical and eco-friendly strategy for cassava pest management. Tetranychus urticae, known as the two-spotted spider mite (TSSM), is a devastating pest in Asian cassava planting countries as well as in China. However, the resistant levels of abundant cassava germplasms to TSSM remains largely unknown.MethodsTo fill this knowledge gap, we conducted screening of 202 cassava germplasm for resistance to TSSM in China based on the classification of mite damage phenotype, under both greenhouse and field conditions.ResultsThe three rounds of large-scale greenhouse experiments had identified two highly resistant (HR) varieties (C1115 and MIANDIAN), five resistant (R) varieties (SC5, SC9, SC15, COLUMBIA-4D and LIMIN) and five highly susceptible (HS) varieties (KU50, BREAD, SC205, TMS60444 and BRA900), besides, these ‘HR’ and ‘R’ varieties would significantly repress the normal development and reproduction of TSSM. In addition, the 12 cassava varieties selected from the greenhouse screening were further subjected to consecutive five years of field validation at Danzhou, Wuming and Baoshan. The seven resistant varieties not only exhibited stable TSSM-resistance performance across the three field environments, but also possessed the same resistant levels as the greenhouse identification, while the resistant varieties SC5 was an exception, which was identified as moderate resistant in Baoshan, indicating the variety-environment interaction may affect its resistance. Furthermore, regional yield estimation suggested that the higher the resistance level was, the better capacity in reducing the yield losses.DiscussionThis study demonstrated that the TSSM-resistant varieties could be considered as ideal materials in mite control or in future breeding programme of mite-resistant cassava plant.https://www.frontiersin.org/articles/10.3389/fpls.2022.1054909/fullManihot esculentaTetranychus urticaeresistancemite pesteco-friendly |
| spellingShingle | Xiao Liang Xiao Liang Qing Chen Qing Chen Ying Liu Ying Liu Chunling Wu Chunling Wu Kaimian Li Mufeng Wu Mufeng Wu Xiaowen Yao Xiaowen Yao Yang Qiao Yang Qiao Yao Zhang Yao Zhang Yue Geng Yue Geng Identification of cassava germplasms resistant to two-spotted spider mite in China: From greenhouse large-scale screening to field validation Frontiers in Plant Science Manihot esculenta Tetranychus urticae resistance mite pest eco-friendly |
| title | Identification of cassava germplasms resistant to two-spotted spider mite in China: From greenhouse large-scale screening to field validation |
| title_full | Identification of cassava germplasms resistant to two-spotted spider mite in China: From greenhouse large-scale screening to field validation |
| title_fullStr | Identification of cassava germplasms resistant to two-spotted spider mite in China: From greenhouse large-scale screening to field validation |
| title_full_unstemmed | Identification of cassava germplasms resistant to two-spotted spider mite in China: From greenhouse large-scale screening to field validation |
| title_short | Identification of cassava germplasms resistant to two-spotted spider mite in China: From greenhouse large-scale screening to field validation |
| title_sort | identification of cassava germplasms resistant to two spotted spider mite in china from greenhouse large scale screening to field validation |
| topic | Manihot esculenta Tetranychus urticae resistance mite pest eco-friendly |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2022.1054909/full |
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