Target-dependent RNA polymerase as universal platform for gene expression control in response to intracellular molecules
Abstract Controlling gene expression in response to specific molecules is an essential technique for regulating cellular functions. However, current platforms with transcription and translation regulators have a limited number of detectable molecules to induce gene expression. Here to address these...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-11-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-023-42802-5 |
| _version_ | 1797558129041342464 |
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| author | Shodai Komatsu Hirohisa Ohno Hirohide Saito |
| author_facet | Shodai Komatsu Hirohisa Ohno Hirohide Saito |
| author_sort | Shodai Komatsu |
| collection | DOAJ |
| description | Abstract Controlling gene expression in response to specific molecules is an essential technique for regulating cellular functions. However, current platforms with transcription and translation regulators have a limited number of detectable molecules to induce gene expression. Here to address these issues, we present a Target-dependent RNA polymerase (TdRNAP) that can induce RNA transcription in response to the intracellular target specifically recognized by single antibody. By substituting the fused antibody, we demonstrate that TdRNAPs respond to a wide variety of molecules, including peptides, proteins, RNA, and small molecules, and produce desired transcripts in human cells. Furthermore, we show that multiple TdRNAPs can construct orthogonal and multilayer genetic circuits. Finally, we apply TdRNAP to achieve cell-specific genome editing that is autonomously triggered by detecting the target gene product. TdRNAP can expand the molecular variety for controlling gene expression and provide the genetic toolbox for bioengineering and future therapeutic applications. |
| first_indexed | 2024-03-10T17:27:14Z |
| format | Article |
| id | doaj.art-2ed9f7cc0c5a4521ab788e0efcf89446 |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-03-10T17:27:14Z |
| publishDate | 2023-11-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-2ed9f7cc0c5a4521ab788e0efcf894462023-11-20T10:08:05ZengNature PortfolioNature Communications2041-17232023-11-0114111110.1038/s41467-023-42802-5Target-dependent RNA polymerase as universal platform for gene expression control in response to intracellular moleculesShodai Komatsu0Hirohisa Ohno1Hirohide Saito2Department of Life Science Frontiers, Center for iPS Cell Research and Application, Kyoto University, 53 Kawahara-cho, Shogoin, Sakyo-kuDepartment of Life Science Frontiers, Center for iPS Cell Research and Application, Kyoto University, 53 Kawahara-cho, Shogoin, Sakyo-kuDepartment of Life Science Frontiers, Center for iPS Cell Research and Application, Kyoto University, 53 Kawahara-cho, Shogoin, Sakyo-kuAbstract Controlling gene expression in response to specific molecules is an essential technique for regulating cellular functions. However, current platforms with transcription and translation regulators have a limited number of detectable molecules to induce gene expression. Here to address these issues, we present a Target-dependent RNA polymerase (TdRNAP) that can induce RNA transcription in response to the intracellular target specifically recognized by single antibody. By substituting the fused antibody, we demonstrate that TdRNAPs respond to a wide variety of molecules, including peptides, proteins, RNA, and small molecules, and produce desired transcripts in human cells. Furthermore, we show that multiple TdRNAPs can construct orthogonal and multilayer genetic circuits. Finally, we apply TdRNAP to achieve cell-specific genome editing that is autonomously triggered by detecting the target gene product. TdRNAP can expand the molecular variety for controlling gene expression and provide the genetic toolbox for bioengineering and future therapeutic applications.https://doi.org/10.1038/s41467-023-42802-5 |
| spellingShingle | Shodai Komatsu Hirohisa Ohno Hirohide Saito Target-dependent RNA polymerase as universal platform for gene expression control in response to intracellular molecules Nature Communications |
| title | Target-dependent RNA polymerase as universal platform for gene expression control in response to intracellular molecules |
| title_full | Target-dependent RNA polymerase as universal platform for gene expression control in response to intracellular molecules |
| title_fullStr | Target-dependent RNA polymerase as universal platform for gene expression control in response to intracellular molecules |
| title_full_unstemmed | Target-dependent RNA polymerase as universal platform for gene expression control in response to intracellular molecules |
| title_short | Target-dependent RNA polymerase as universal platform for gene expression control in response to intracellular molecules |
| title_sort | target dependent rna polymerase as universal platform for gene expression control in response to intracellular molecules |
| url | https://doi.org/10.1038/s41467-023-42802-5 |
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