Intraocular liver spheroids for non-invasive high-resolution in vivo monitoring of liver cell function
Abstract Longitudinal monitoring of liver function in vivo is hindered by the lack of high-resolution non-invasive imaging techniques. Using the anterior chamber of the mouse eye as a transplantation site, we have established a platform for longitudinal in vivo imaging of liver spheroids at cellular...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2024-01-01
|
| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-024-45122-4 |
| _version_ | 1797276457862430720 |
|---|---|
| author | Francesca Lazzeri-Barcelo Nuria Oliva-Vilarnau Marion Baniol Barbara Leibiger Olaf Bergmann Volker M. Lauschke Ingo B. Leibiger Noah Moruzzi Per-Olof Berggren |
| author_facet | Francesca Lazzeri-Barcelo Nuria Oliva-Vilarnau Marion Baniol Barbara Leibiger Olaf Bergmann Volker M. Lauschke Ingo B. Leibiger Noah Moruzzi Per-Olof Berggren |
| author_sort | Francesca Lazzeri-Barcelo |
| collection | DOAJ |
| description | Abstract Longitudinal monitoring of liver function in vivo is hindered by the lack of high-resolution non-invasive imaging techniques. Using the anterior chamber of the mouse eye as a transplantation site, we have established a platform for longitudinal in vivo imaging of liver spheroids at cellular resolution. Transplanted liver spheroids engraft on the iris, become vascularized and innervated, retain hepatocyte-specific and liver-like features and can be studied by in vivo confocal microscopy. Employing fluorescent probes administered intravenously or spheroids formed from reporter mice, we showcase the potential use of this platform for monitoring hepatocyte cell cycle activity, bile secretion and lipoprotein uptake. Moreover, we show that hepatic lipid accumulation during diet-induced hepatosteatosis is mirrored in intraocular in vivo grafts. Here, we show a new technology which provides a crucial and unique tool to study liver physiology and disease progression in pre-clinical and basic research. |
| first_indexed | 2024-03-07T15:28:30Z |
| format | Article |
| id | doaj.art-2eda5aaab39e4b1b93164e67176e1475 |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-03-07T15:28:30Z |
| publishDate | 2024-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-2eda5aaab39e4b1b93164e67176e14752024-03-05T16:34:07ZengNature PortfolioNature Communications2041-17232024-01-0115111410.1038/s41467-024-45122-4Intraocular liver spheroids for non-invasive high-resolution in vivo monitoring of liver cell functionFrancesca Lazzeri-Barcelo0Nuria Oliva-Vilarnau1Marion Baniol2Barbara Leibiger3Olaf Bergmann4Volker M. Lauschke5Ingo B. Leibiger6Noah Moruzzi7Per-Olof Berggren8The Rolf Luft Research Center for Diabetes and Endocrinology, Karolinska InstitutetDepartment of Physiology and Pharmacology, Karolinska InstitutetDepartment of Cell and Molecular Biology, Karolinska InstitutetThe Rolf Luft Research Center for Diabetes and Endocrinology, Karolinska InstitutetDepartment of Cell and Molecular Biology, Karolinska InstitutetDepartment of Physiology and Pharmacology, Karolinska InstitutetThe Rolf Luft Research Center for Diabetes and Endocrinology, Karolinska InstitutetThe Rolf Luft Research Center for Diabetes and Endocrinology, Karolinska InstitutetThe Rolf Luft Research Center for Diabetes and Endocrinology, Karolinska InstitutetAbstract Longitudinal monitoring of liver function in vivo is hindered by the lack of high-resolution non-invasive imaging techniques. Using the anterior chamber of the mouse eye as a transplantation site, we have established a platform for longitudinal in vivo imaging of liver spheroids at cellular resolution. Transplanted liver spheroids engraft on the iris, become vascularized and innervated, retain hepatocyte-specific and liver-like features and can be studied by in vivo confocal microscopy. Employing fluorescent probes administered intravenously or spheroids formed from reporter mice, we showcase the potential use of this platform for monitoring hepatocyte cell cycle activity, bile secretion and lipoprotein uptake. Moreover, we show that hepatic lipid accumulation during diet-induced hepatosteatosis is mirrored in intraocular in vivo grafts. Here, we show a new technology which provides a crucial and unique tool to study liver physiology and disease progression in pre-clinical and basic research.https://doi.org/10.1038/s41467-024-45122-4 |
| spellingShingle | Francesca Lazzeri-Barcelo Nuria Oliva-Vilarnau Marion Baniol Barbara Leibiger Olaf Bergmann Volker M. Lauschke Ingo B. Leibiger Noah Moruzzi Per-Olof Berggren Intraocular liver spheroids for non-invasive high-resolution in vivo monitoring of liver cell function Nature Communications |
| title | Intraocular liver spheroids for non-invasive high-resolution in vivo monitoring of liver cell function |
| title_full | Intraocular liver spheroids for non-invasive high-resolution in vivo monitoring of liver cell function |
| title_fullStr | Intraocular liver spheroids for non-invasive high-resolution in vivo monitoring of liver cell function |
| title_full_unstemmed | Intraocular liver spheroids for non-invasive high-resolution in vivo monitoring of liver cell function |
| title_short | Intraocular liver spheroids for non-invasive high-resolution in vivo monitoring of liver cell function |
| title_sort | intraocular liver spheroids for non invasive high resolution in vivo monitoring of liver cell function |
| url | https://doi.org/10.1038/s41467-024-45122-4 |
| work_keys_str_mv | AT francescalazzeribarcelo intraocularliverspheroidsfornoninvasivehighresolutioninvivomonitoringoflivercellfunction AT nuriaolivavilarnau intraocularliverspheroidsfornoninvasivehighresolutioninvivomonitoringoflivercellfunction AT marionbaniol intraocularliverspheroidsfornoninvasivehighresolutioninvivomonitoringoflivercellfunction AT barbaraleibiger intraocularliverspheroidsfornoninvasivehighresolutioninvivomonitoringoflivercellfunction AT olafbergmann intraocularliverspheroidsfornoninvasivehighresolutioninvivomonitoringoflivercellfunction AT volkermlauschke intraocularliverspheroidsfornoninvasivehighresolutioninvivomonitoringoflivercellfunction AT ingobleibiger intraocularliverspheroidsfornoninvasivehighresolutioninvivomonitoringoflivercellfunction AT noahmoruzzi intraocularliverspheroidsfornoninvasivehighresolutioninvivomonitoringoflivercellfunction AT perolofberggren intraocularliverspheroidsfornoninvasivehighresolutioninvivomonitoringoflivercellfunction |