Fenton-Mediated Chlorophenol Degradation by Iron-Reducing Compounds Isolated from Endophytic Fungi in Atacama Puna Plateau <i>Lecanicillium</i> ATA01
Low-molecular-mass iron-reducing compounds (IRCs) were produced by entomopathogenic endophytic fungi <i>Lecanicillium</i> sp. ATA01 in liquid cultures. The extracellular hydrophilic extract contained three IRCs formed by peptides, iron and phenolate structures with molecular masses of 12...
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| Format: | Article |
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MDPI AG
2022-03-01
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| Series: | Fermentation |
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| Online Access: | https://www.mdpi.com/2311-5637/8/4/147 |
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| author | Rómulo Oses-Pedraza Estefanía Bonnail Paris Lavin Cristina Purcarea Manuel Alarcón Danae Irribarren-Riquelme Jaime Rodríguez |
| author_facet | Rómulo Oses-Pedraza Estefanía Bonnail Paris Lavin Cristina Purcarea Manuel Alarcón Danae Irribarren-Riquelme Jaime Rodríguez |
| author_sort | Rómulo Oses-Pedraza |
| collection | DOAJ |
| description | Low-molecular-mass iron-reducing compounds (IRCs) were produced by entomopathogenic endophytic fungi <i>Lecanicillium</i> sp. ATA01 in liquid cultures. The extracellular hydrophilic extract contained three IRCs formed by peptides, iron and phenolate structures with molecular masses of 1207, 567 and 550 Da. These compounds were able to chelate and mediate the reduction of Fe<sup>+3</sup> to Fe<sup>+2</sup> and oxidized recalcitrant lignin-model substrates such as veratryl alcohol (VA), 2,6-dimethoxyphenol (DMP), and 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid (ABTS) with or without hydrogen peroxide. Besides, IRCs can promote the degradation of chlorophenols. The maximal degradation of p-chlorophenol, 2,4-dichlorophenol, 2,4,6-trichlorophenol, and pentachlorophenol was conducted at optimal degradation conditions for IRCs (pH 3.5, iron 100 mM, and H<sub>2</sub>O<sub>2</sub> 10 mM). Furthermore, Fenton-like reactions using the synthetic iron chelates DTPA and EDTA and free Fe<sup>+2</sup> and Fe<sup>+3</sup> were also carried out in order to compare with the reaction mediated by IRCs. The ferric IRCs displayed the ability to enhance the hydroxylation of chlorophenols as a part of a degradation mechanism of the IRC-assisted Fenton reaction. The complexed iron was more efficient than free iron in the Fenton-like reaction, and between them, the fungal chelates were more efficient than the synthetic mill chelates. |
| first_indexed | 2024-03-09T10:37:02Z |
| format | Article |
| id | doaj.art-2edda7e5170843608265d607774a0646 |
| institution | Directory Open Access Journal |
| issn | 2311-5637 |
| language | English |
| last_indexed | 2024-03-09T10:37:02Z |
| publishDate | 2022-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Fermentation |
| spelling | doaj.art-2edda7e5170843608265d607774a06462023-12-01T20:51:49ZengMDPI AGFermentation2311-56372022-03-018414710.3390/fermentation8040147Fenton-Mediated Chlorophenol Degradation by Iron-Reducing Compounds Isolated from Endophytic Fungi in Atacama Puna Plateau <i>Lecanicillium</i> ATA01Rómulo Oses-Pedraza0Estefanía Bonnail1Paris Lavin2Cristina Purcarea3Manuel Alarcón4Danae Irribarren-Riquelme5Jaime Rodríguez6Centro Regional de Investigación y Desarrollo Sustentable de Atacama (CRIDESAT), Universidad de Atacama, Av. Copayapu Nr 485, Copiapó 1530000, ChileCentro de Investigaciones Costeras, Universidad de Atacama (CIC-UDA), Av. Copayapu 485, Copiapó 1530000, ChileDepartamento de Biotecnología, Facultad de Ciencias del Mar y Recursos Biológicos, Universidad de Antofagasta, Antofagasta 1240000, ChileDepartment of Microbiology, Institute of Biology Bucharest of Romanian Academy, Splaiul Independentei, 296, 060031 Bucharest, RomaniaInstituto de Acuicultura, Universidad Austral de Chile, Puerto Montt 5480000, ChileEnvironmental Microbiology and Biotechnology Unit Zenobia Group SpA, Flores Millan 1350, Chillán 3780000, ChileCenter of Biotechnology, Renewable Resources Laboratory, Universidad de Concepción, Casilla 160-C, Concepción 4030000, ChileLow-molecular-mass iron-reducing compounds (IRCs) were produced by entomopathogenic endophytic fungi <i>Lecanicillium</i> sp. ATA01 in liquid cultures. The extracellular hydrophilic extract contained three IRCs formed by peptides, iron and phenolate structures with molecular masses of 1207, 567 and 550 Da. These compounds were able to chelate and mediate the reduction of Fe<sup>+3</sup> to Fe<sup>+2</sup> and oxidized recalcitrant lignin-model substrates such as veratryl alcohol (VA), 2,6-dimethoxyphenol (DMP), and 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid (ABTS) with or without hydrogen peroxide. Besides, IRCs can promote the degradation of chlorophenols. The maximal degradation of p-chlorophenol, 2,4-dichlorophenol, 2,4,6-trichlorophenol, and pentachlorophenol was conducted at optimal degradation conditions for IRCs (pH 3.5, iron 100 mM, and H<sub>2</sub>O<sub>2</sub> 10 mM). Furthermore, Fenton-like reactions using the synthetic iron chelates DTPA and EDTA and free Fe<sup>+2</sup> and Fe<sup>+3</sup> were also carried out in order to compare with the reaction mediated by IRCs. The ferric IRCs displayed the ability to enhance the hydroxylation of chlorophenols as a part of a degradation mechanism of the IRC-assisted Fenton reaction. The complexed iron was more efficient than free iron in the Fenton-like reaction, and between them, the fungal chelates were more efficient than the synthetic mill chelates.https://www.mdpi.com/2311-5637/8/4/147endophytic fungi<i>Lecanicilliun</i>iron-reducing compoundsFenton-like reactionchlorophenolhydroxylation |
| spellingShingle | Rómulo Oses-Pedraza Estefanía Bonnail Paris Lavin Cristina Purcarea Manuel Alarcón Danae Irribarren-Riquelme Jaime Rodríguez Fenton-Mediated Chlorophenol Degradation by Iron-Reducing Compounds Isolated from Endophytic Fungi in Atacama Puna Plateau <i>Lecanicillium</i> ATA01 Fermentation endophytic fungi <i>Lecanicilliun</i> iron-reducing compounds Fenton-like reaction chlorophenol hydroxylation |
| title | Fenton-Mediated Chlorophenol Degradation by Iron-Reducing Compounds Isolated from Endophytic Fungi in Atacama Puna Plateau <i>Lecanicillium</i> ATA01 |
| title_full | Fenton-Mediated Chlorophenol Degradation by Iron-Reducing Compounds Isolated from Endophytic Fungi in Atacama Puna Plateau <i>Lecanicillium</i> ATA01 |
| title_fullStr | Fenton-Mediated Chlorophenol Degradation by Iron-Reducing Compounds Isolated from Endophytic Fungi in Atacama Puna Plateau <i>Lecanicillium</i> ATA01 |
| title_full_unstemmed | Fenton-Mediated Chlorophenol Degradation by Iron-Reducing Compounds Isolated from Endophytic Fungi in Atacama Puna Plateau <i>Lecanicillium</i> ATA01 |
| title_short | Fenton-Mediated Chlorophenol Degradation by Iron-Reducing Compounds Isolated from Endophytic Fungi in Atacama Puna Plateau <i>Lecanicillium</i> ATA01 |
| title_sort | fenton mediated chlorophenol degradation by iron reducing compounds isolated from endophytic fungi in atacama puna plateau i lecanicillium i ata01 |
| topic | endophytic fungi <i>Lecanicilliun</i> iron-reducing compounds Fenton-like reaction chlorophenol hydroxylation |
| url | https://www.mdpi.com/2311-5637/8/4/147 |
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