Characterization of a novel psyllid-transmitted waikavirus in carrots
Carrots collected from the Western Negev region in Israel during the winter of 2019 showed disease symptoms of chlorosis, leaf curling, a loss of apical dominance, and multiple lateral roots that were not associated with known pathogens of the carrot yellows disease. Symptomatic carrots were studied...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-10-01
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| Series: | Virus Research |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0168170223001545 |
| _version_ | 1797736901610831872 |
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| author | Ariel Shushan Neta Luria Oded Lachman Noa Sela Orly Laskar Eduard Belausov Elisheva Smith Aviv Dombrovsky |
| author_facet | Ariel Shushan Neta Luria Oded Lachman Noa Sela Orly Laskar Eduard Belausov Elisheva Smith Aviv Dombrovsky |
| author_sort | Ariel Shushan |
| collection | DOAJ |
| description | Carrots collected from the Western Negev region in Israel during the winter of 2019 showed disease symptoms of chlorosis, leaf curling, a loss of apical dominance, and multiple lateral roots that were not associated with known pathogens of the carrot yellows disease. Symptomatic carrots were studied for a possible involvement of plant viruses in disease manifestations using high throughput sequencing analyses. The results revealed the presence of a waikavirus, sharing a ∼70% nucleotide sequence identity with Waikavirus genus members. Virions purified from waikavirus-positive carrots were visualized by transmission electron microscopy, showing icosahedral particle diameter of ∼28 nm. The genome sequence was validated by overlapping amplicons by designed 12 primer sets. A complete genome sequence was achieved by rapid amplification of cDNA ends (RACE) for sequencing the 5′ end, and RT-PCR with oligo dT for sequencing the 3′ end. The genome encodes a single large ORF, characteristic of waikaviruses. Aligning the waikavirus-deduced amino-acid sequence with other waikavirus species at the Pro-Pol region, a conserved sequence between the putative proteinase and the RNA-dependent RNA polymerase, showed a ∼40% identity, indicating the identification of a new waikavirus species. The amino-acid sequence of the three coat proteins and cleavage sites were experimentally determined by liquid chromatography-mass spectrometry. A phylogenetic analysis based on the Pro-Pol region revealed that the new waikavirus clusters with persimmon waikavirus and actinidia yellowing virus 1. The new waikavirus genome was localized in the phloem of waikavirus-infected carrots. The virus was transmitted to carrot and coriander plants by the psyllid Bactericera trigonica Hodkinson (Hemiptera: Triozidae). |
| first_indexed | 2024-03-12T13:20:12Z |
| format | Article |
| id | doaj.art-2edeba161d8e480db13178ac21f059b5 |
| institution | Directory Open Access Journal |
| issn | 1872-7492 |
| language | English |
| last_indexed | 2024-03-12T13:20:12Z |
| publishDate | 2023-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Virus Research |
| spelling | doaj.art-2edeba161d8e480db13178ac21f059b52023-08-26T04:42:09ZengElsevierVirus Research1872-74922023-10-01335199192Characterization of a novel psyllid-transmitted waikavirus in carrotsAriel Shushan0Neta Luria1Oded Lachman2Noa Sela3Orly Laskar4Eduard Belausov5Elisheva Smith6Aviv Dombrovsky7Department of Plant Pathology and Weed Research, Agricultural Research Organization—The Volcani Center, 68 HaMaccabim Road, P.O.B 15159, Rishon LeTsiyon 7528809, Israel; The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of University of Jerusalem, Rehovot 761001, IsraelDepartment of Plant Pathology and Weed Research, Agricultural Research Organization—The Volcani Center, 68 HaMaccabim Road, P.O.B 15159, Rishon LeTsiyon 7528809, IsraelDepartment of Plant Pathology and Weed Research, Agricultural Research Organization—The Volcani Center, 68 HaMaccabim Road, P.O.B 15159, Rishon LeTsiyon 7528809, IsraelBioinformatics Unit, Agricultural Research Organization—The Volcani Center, 68 HaMaccabim Road, P.O.B 15159, Rishon LeZion 7505101, IsraelDepartment of Infectious Diseases, Israel Institute for Biological Research, P.O.B 19, Ness Ziona 74100, IsraelDepartment of Ornamental Plants and Agricultural Biotechnology, Agricultural Research Organization, The Volcani Center, 68 HaMaccabim Road, P.O.B 15159, Rishon LeZion 7505101, IsraelDepartment of Plant Pathology and Weed Research, Agricultural Research Organization—The Volcani Center, 68 HaMaccabim Road, P.O.B 15159, Rishon LeTsiyon 7528809, IsraelDepartment of Plant Pathology and Weed Research, Agricultural Research Organization—The Volcani Center, 68 HaMaccabim Road, P.O.B 15159, Rishon LeTsiyon 7528809, Israel; Corresponding author.Carrots collected from the Western Negev region in Israel during the winter of 2019 showed disease symptoms of chlorosis, leaf curling, a loss of apical dominance, and multiple lateral roots that were not associated with known pathogens of the carrot yellows disease. Symptomatic carrots were studied for a possible involvement of plant viruses in disease manifestations using high throughput sequencing analyses. The results revealed the presence of a waikavirus, sharing a ∼70% nucleotide sequence identity with Waikavirus genus members. Virions purified from waikavirus-positive carrots were visualized by transmission electron microscopy, showing icosahedral particle diameter of ∼28 nm. The genome sequence was validated by overlapping amplicons by designed 12 primer sets. A complete genome sequence was achieved by rapid amplification of cDNA ends (RACE) for sequencing the 5′ end, and RT-PCR with oligo dT for sequencing the 3′ end. The genome encodes a single large ORF, characteristic of waikaviruses. Aligning the waikavirus-deduced amino-acid sequence with other waikavirus species at the Pro-Pol region, a conserved sequence between the putative proteinase and the RNA-dependent RNA polymerase, showed a ∼40% identity, indicating the identification of a new waikavirus species. The amino-acid sequence of the three coat proteins and cleavage sites were experimentally determined by liquid chromatography-mass spectrometry. A phylogenetic analysis based on the Pro-Pol region revealed that the new waikavirus clusters with persimmon waikavirus and actinidia yellowing virus 1. The new waikavirus genome was localized in the phloem of waikavirus-infected carrots. The virus was transmitted to carrot and coriander plants by the psyllid Bactericera trigonica Hodkinson (Hemiptera: Triozidae).http://www.sciencedirect.com/science/article/pii/S0168170223001545Bactericera trigonicaCarrot yellows diseasePhloem-limited |
| spellingShingle | Ariel Shushan Neta Luria Oded Lachman Noa Sela Orly Laskar Eduard Belausov Elisheva Smith Aviv Dombrovsky Characterization of a novel psyllid-transmitted waikavirus in carrots Virus Research Bactericera trigonica Carrot yellows disease Phloem-limited |
| title | Characterization of a novel psyllid-transmitted waikavirus in carrots |
| title_full | Characterization of a novel psyllid-transmitted waikavirus in carrots |
| title_fullStr | Characterization of a novel psyllid-transmitted waikavirus in carrots |
| title_full_unstemmed | Characterization of a novel psyllid-transmitted waikavirus in carrots |
| title_short | Characterization of a novel psyllid-transmitted waikavirus in carrots |
| title_sort | characterization of a novel psyllid transmitted waikavirus in carrots |
| topic | Bactericera trigonica Carrot yellows disease Phloem-limited |
| url | http://www.sciencedirect.com/science/article/pii/S0168170223001545 |
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