Anti-proliferative Effects of a Coumarin Benjaminin on Four Human Cancer Cell Lines
Cancer is a major health issue around the globe. Chemotherapy and radiation therapy are no longer the standard treatments for patients as these remedies lead to many adverse effects. Thus, the development of alternative effective drugs from lead compounds, especially natural products is necessary. T...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Universitas Indonesia
2020-04-01
|
| Series: | Pharmaceutical Sciences and Research |
| Subjects: | |
| Online Access: | https://scholarhub.ui.ac.id/psr/vol7/iss1/7/ |
| _version_ | 1819075549180461056 |
|---|---|
| author | Siau Hui Mah Soek Sin Teh Ahmad Azri Fitri Ismail Gwendoline Cheng Lian Ee |
| author_facet | Siau Hui Mah Soek Sin Teh Ahmad Azri Fitri Ismail Gwendoline Cheng Lian Ee |
| author_sort | Siau Hui Mah |
| collection | DOAJ |
| description | Cancer is a major health issue around the globe. Chemotherapy and radiation therapy are no longer the standard treatments for patients as these remedies lead to many adverse effects. Thus, the development of alternative effective drugs from lead compounds, especially natural products is necessary. This study aimed to isolate cytotoxic phytochemical constituents from a plant Calophyllum inophyllum, which has been used as traditional medicine since ancient time. The root of C. inophyllum was extracted and subjected for phytochemical constituent isolation. A coumarin, benjaminin (1) was isolated successfully from the chloroform extract and its structural elucidation was performed by spectroscopy analyses of MS, IR and NMR. The anti-proliferative effect of 1 on four cancer cell lines, leukemia (K562), stomach (SNU-1), liver (Hep-G2) and lung (NCI-H23) cancers were evaluated by using colorimetric MTT assay. Benjaminin (1) exhibited cytotoxic effects towards all cancer cells and showed the strongest inhibition towards SNU-1 cell proliferation with an IC50 value of 70.42 μM. The outcome of this study revealed that 1 is a potential cytotoxic lead compound that could be further developed into anti-cancer drug. Thus, the study on the structure-activity relationship of 1 is highly recommended in due course to improve its anti-proliferative effects on cancer cells. |
| first_indexed | 2024-12-21T18:27:10Z |
| format | Article |
| id | doaj.art-2ee0bb72ecfa45b9a943523bbbe4c525 |
| institution | Directory Open Access Journal |
| issn | 2407-2354 2477-0612 |
| language | English |
| last_indexed | 2024-12-21T18:27:10Z |
| publishDate | 2020-04-01 |
| publisher | Universitas Indonesia |
| record_format | Article |
| series | Pharmaceutical Sciences and Research |
| spelling | doaj.art-2ee0bb72ecfa45b9a943523bbbe4c5252022-12-21T18:54:22ZengUniversitas IndonesiaPharmaceutical Sciences and Research2407-23542477-06122020-04-01715156https://doi.org/10.7454/psr.v7i1.1057Anti-proliferative Effects of a Coumarin Benjaminin on Four Human Cancer Cell LinesSiau Hui Mah0Soek Sin Teh1Ahmad Azri Fitri Ismail2Gwendoline Cheng Lian Ee3School of Biosciences, Taylor’s University, Lakeside Campus, 47500 Subang Jaya, Selangor, MalaysiaEngineering and Processing Division, Energy and Environment Unit, Malaysian Palm Oil Board, Kajang, Malaysian Palm Oil Board, 43000 Kajang, Selangor, MalaysiaDepartment of Chemistry, Faculty of Science, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, MalaysiaDepartment of Chemistry, Faculty of Science, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, MalaysiaCancer is a major health issue around the globe. Chemotherapy and radiation therapy are no longer the standard treatments for patients as these remedies lead to many adverse effects. Thus, the development of alternative effective drugs from lead compounds, especially natural products is necessary. This study aimed to isolate cytotoxic phytochemical constituents from a plant Calophyllum inophyllum, which has been used as traditional medicine since ancient time. The root of C. inophyllum was extracted and subjected for phytochemical constituent isolation. A coumarin, benjaminin (1) was isolated successfully from the chloroform extract and its structural elucidation was performed by spectroscopy analyses of MS, IR and NMR. The anti-proliferative effect of 1 on four cancer cell lines, leukemia (K562), stomach (SNU-1), liver (Hep-G2) and lung (NCI-H23) cancers were evaluated by using colorimetric MTT assay. Benjaminin (1) exhibited cytotoxic effects towards all cancer cells and showed the strongest inhibition towards SNU-1 cell proliferation with an IC50 value of 70.42 μM. The outcome of this study revealed that 1 is a potential cytotoxic lead compound that could be further developed into anti-cancer drug. Thus, the study on the structure-activity relationship of 1 is highly recommended in due course to improve its anti-proliferative effects on cancer cells.https://scholarhub.ui.ac.id/psr/vol7/iss1/7/calophyllum inophyllumcytotoxicisolationmtt assay |
| spellingShingle | Siau Hui Mah Soek Sin Teh Ahmad Azri Fitri Ismail Gwendoline Cheng Lian Ee Anti-proliferative Effects of a Coumarin Benjaminin on Four Human Cancer Cell Lines Pharmaceutical Sciences and Research calophyllum inophyllum cytotoxic isolation mtt assay |
| title | Anti-proliferative Effects of a Coumarin Benjaminin on Four Human Cancer Cell Lines |
| title_full | Anti-proliferative Effects of a Coumarin Benjaminin on Four Human Cancer Cell Lines |
| title_fullStr | Anti-proliferative Effects of a Coumarin Benjaminin on Four Human Cancer Cell Lines |
| title_full_unstemmed | Anti-proliferative Effects of a Coumarin Benjaminin on Four Human Cancer Cell Lines |
| title_short | Anti-proliferative Effects of a Coumarin Benjaminin on Four Human Cancer Cell Lines |
| title_sort | anti proliferative effects of a coumarin benjaminin on four human cancer cell lines |
| topic | calophyllum inophyllum cytotoxic isolation mtt assay |
| url | https://scholarhub.ui.ac.id/psr/vol7/iss1/7/ |
| work_keys_str_mv | AT siauhuimah antiproliferativeeffectsofacoumarinbenjamininonfourhumancancercelllines AT soeksinteh antiproliferativeeffectsofacoumarinbenjamininonfourhumancancercelllines AT ahmadazrifitriismail antiproliferativeeffectsofacoumarinbenjamininonfourhumancancercelllines AT gwendolinechenglianee antiproliferativeeffectsofacoumarinbenjamininonfourhumancancercelllines |