Biophoton Imaging: A Nondestructive Method for Assaying R Gene Responses
Plant disease resistance (R) proteins of the nucleotide binding-leucine rich repeat class are responsible for pathogen recognition and activation of defense signaling networks leading to the hypersensitive response (HR). Genetically, R-protein signaling appears to be integrated through a limited set...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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The American Phytopathological Society
2005-02-01
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| Series: | Molecular Plant-Microbe Interactions |
| Subjects: | |
| Online Access: | https://apsjournals.apsnet.org/doi/10.1094/MPMI-18-0095 |
| _version_ | 1818757325591150592 |
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| author | Mark Bennett Monaz Mehta Murray Grant |
| author_facet | Mark Bennett Monaz Mehta Murray Grant |
| author_sort | Mark Bennett |
| collection | DOAJ |
| description | Plant disease resistance (R) proteins of the nucleotide binding-leucine rich repeat class are responsible for pathogen recognition and activation of defense signaling networks leading to the hypersensitive response (HR). Genetically, R-protein signaling appears to be integrated through a limited set of common downstream components. However, the timing of development of visible HR is unique to individual R proteins. By utilizing the phenomena of ultraweak photon emission from leaves undergoing an incompatible interacttion, a powerful nondestructive and facile assay is described to compare timing of defense responses elicited by different R proteins. We demonstrate that ultraweak photon emission, or “biophoton generation,” is demonstrated to be associated with hypersensitive cell death. Biophoton emission requires an intact R signaling network and increases in cytosolic calcium and nitric oxide, but elevated reactive oxygen species are not necessary. Importantly, the assay is robust and applicable to a range of incompatible interactions in various plant species. The ability to assay R responses nondestructively in real time and a chosen genetic background makes this technique amenable to subtle genetic dissection of plant defense responses. |
| first_indexed | 2024-12-18T06:09:09Z |
| format | Article |
| id | doaj.art-2ee3e1410bb3419aa7f931797bf62444 |
| institution | Directory Open Access Journal |
| issn | 0894-0282 1943-7706 |
| language | English |
| last_indexed | 2024-12-18T06:09:09Z |
| publishDate | 2005-02-01 |
| publisher | The American Phytopathological Society |
| record_format | Article |
| series | Molecular Plant-Microbe Interactions |
| spelling | doaj.art-2ee3e1410bb3419aa7f931797bf624442022-12-21T21:18:28ZengThe American Phytopathological SocietyMolecular Plant-Microbe Interactions0894-02821943-77062005-02-011829510210.1094/MPMI-18-0095Biophoton Imaging: A Nondestructive Method for Assaying R Gene ResponsesMark BennettMonaz MehtaMurray GrantPlant disease resistance (R) proteins of the nucleotide binding-leucine rich repeat class are responsible for pathogen recognition and activation of defense signaling networks leading to the hypersensitive response (HR). Genetically, R-protein signaling appears to be integrated through a limited set of common downstream components. However, the timing of development of visible HR is unique to individual R proteins. By utilizing the phenomena of ultraweak photon emission from leaves undergoing an incompatible interacttion, a powerful nondestructive and facile assay is described to compare timing of defense responses elicited by different R proteins. We demonstrate that ultraweak photon emission, or “biophoton generation,” is demonstrated to be associated with hypersensitive cell death. Biophoton emission requires an intact R signaling network and increases in cytosolic calcium and nitric oxide, but elevated reactive oxygen species are not necessary. Importantly, the assay is robust and applicable to a range of incompatible interactions in various plant species. The ability to assay R responses nondestructively in real time and a chosen genetic background makes this technique amenable to subtle genetic dissection of plant defense responses.https://apsjournals.apsnet.org/doi/10.1094/MPMI-18-0095RPM1Pseudomonas syringae |
| spellingShingle | Mark Bennett Monaz Mehta Murray Grant Biophoton Imaging: A Nondestructive Method for Assaying R Gene Responses Molecular Plant-Microbe Interactions RPM1 Pseudomonas syringae |
| title | Biophoton Imaging: A Nondestructive Method for Assaying R Gene Responses |
| title_full | Biophoton Imaging: A Nondestructive Method for Assaying R Gene Responses |
| title_fullStr | Biophoton Imaging: A Nondestructive Method for Assaying R Gene Responses |
| title_full_unstemmed | Biophoton Imaging: A Nondestructive Method for Assaying R Gene Responses |
| title_short | Biophoton Imaging: A Nondestructive Method for Assaying R Gene Responses |
| title_sort | biophoton imaging a nondestructive method for assaying r gene responses |
| topic | RPM1 Pseudomonas syringae |
| url | https://apsjournals.apsnet.org/doi/10.1094/MPMI-18-0095 |
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