Discovery on Antibiotic Resistance Patterns of Vibrio parahaemolyticus in Selangor Reveals Carbapenemase Producing Vibrio parahaemolyticus in Marine and Freshwater Fish
Vibrio parahaemolyticus, a Gram-negative halophilic bacterium is often associated with fish and fishery products, thus causing gastroenteritis in humans upon ingestion of contaminated food. V. parahaemolyticus has become a globally well-known pathogen with yearly reported cases in many countries. Th...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2018-10-01
|
| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/article/10.3389/fmicb.2018.02513/full |
| _version_ | 1819171814466650112 |
|---|---|
| author | Learn-Han Lee Learn-Han Lee Learn-Han Lee Nurul-Syakima Ab Mutalib Jodi Woan-Fei Law Jodi Woan-Fei Law Sunny Hei Wong Vengadesh Letchumanan Vengadesh Letchumanan |
| author_facet | Learn-Han Lee Learn-Han Lee Learn-Han Lee Nurul-Syakima Ab Mutalib Jodi Woan-Fei Law Jodi Woan-Fei Law Sunny Hei Wong Vengadesh Letchumanan Vengadesh Letchumanan |
| author_sort | Learn-Han Lee |
| collection | DOAJ |
| description | Vibrio parahaemolyticus, a Gram-negative halophilic bacterium is often associated with fish and fishery products, thus causing gastroenteritis in humans upon ingestion of contaminated food. V. parahaemolyticus has become a globally well-known pathogen with yearly reported cases in many countries. This study aimed to discover the antibiotic resistance patterns of V. parahaemolyticus as well as detect Carbapenem resistant isolates from marine and freshwater fish in Selangor. A total of 240 freshwater and marine fish samples collected from wet market and supermarket in Selangor were tested for the presence of V. parahaemolyticus. All the fish samples were determined positive for V. parahaemolyticus using conventional microbiological culture-based method. The toxR gene were detected via polymerase chain reaction (PCR) in 165/240 (69%) isolates. The two-virulence factor of V. parahaemolyticus, thermostable direct hemolysin (tdh) and TDH-related hemolysin (trh) was screened via PCR. As such, four isolates were trh+and none were tdh+. Majority of the isolates presented high resistance to ampicillin (88%), amikacin (64%), and kanamycin (50%). In addition, this study identified 19-imipenem resistant isolates isolated from freshwater and marine fish samples. Further analysis of these 19-imipenem resistant isolates revealed that the resistance toward imipenem was plasmid mediated after plasmid curing assay. The multiple antibiotics resistance index was >0.2 for 70% of the isolates. In summary, the results confirm the presence of V. parahaemolyticus in freshwater and marine fish samples in Selangor, Malaysia. To our best knowledge, this is the first report discovering the antibiotic resistant patterns and Carbapenem-resistant isolates of V. parahaemolyticus isolated from marine and freshwater fish samples in Selangor. |
| first_indexed | 2024-12-22T19:57:16Z |
| format | Article |
| id | doaj.art-2ee588662bb84dd784ef3d2e3132f181 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-22T19:57:16Z |
| publishDate | 2018-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-2ee588662bb84dd784ef3d2e3132f1812022-12-21T18:14:24ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2018-10-01910.3389/fmicb.2018.02513407883Discovery on Antibiotic Resistance Patterns of Vibrio parahaemolyticus in Selangor Reveals Carbapenemase Producing Vibrio parahaemolyticus in Marine and Freshwater FishLearn-Han Lee0Learn-Han Lee1Learn-Han Lee2Nurul-Syakima Ab Mutalib3Jodi Woan-Fei Law4Jodi Woan-Fei Law5Sunny Hei Wong6Vengadesh Letchumanan7Vengadesh Letchumanan8Novel Bacteria and Drug Discovery Research Group, Biomedicine Research Advancement Centre, School of Pharmacy, Monash University Malaysia, Bandar Sunway, MalaysiaBiomedical Research Laboratory, Jeffrey Cheah School of Medicine and Health Sciences, Monash University Malaysia, Bandar Sunway, MalaysiaCenter of Health Outcomes Research and Therapeutic Safety, School of Pharmaceutical Sciences, University of Phayao, Phayao, ThailandUKM Medical Molecular Biology Institute, UKM Medical Centre, National University of Malaysia, Bangi, MalaysiaNovel Bacteria and Drug Discovery Research Group, Biomedicine Research Advancement Centre, School of Pharmacy, Monash University Malaysia, Bandar Sunway, MalaysiaBiomedical Research Laboratory, Jeffrey Cheah School of Medicine and Health Sciences, Monash University Malaysia, Bandar Sunway, MalaysiaDepartment of Medicine and Therapeutics, Li Ka Shing Institute of Health Sciences, The Chinese University of Hong Kong, Shatin, Hong KongNovel Bacteria and Drug Discovery Research Group, Biomedicine Research Advancement Centre, School of Pharmacy, Monash University Malaysia, Bandar Sunway, MalaysiaBiomedical Research Laboratory, Jeffrey Cheah School of Medicine and Health Sciences, Monash University Malaysia, Bandar Sunway, MalaysiaVibrio parahaemolyticus, a Gram-negative halophilic bacterium is often associated with fish and fishery products, thus causing gastroenteritis in humans upon ingestion of contaminated food. V. parahaemolyticus has become a globally well-known pathogen with yearly reported cases in many countries. This study aimed to discover the antibiotic resistance patterns of V. parahaemolyticus as well as detect Carbapenem resistant isolates from marine and freshwater fish in Selangor. A total of 240 freshwater and marine fish samples collected from wet market and supermarket in Selangor were tested for the presence of V. parahaemolyticus. All the fish samples were determined positive for V. parahaemolyticus using conventional microbiological culture-based method. The toxR gene were detected via polymerase chain reaction (PCR) in 165/240 (69%) isolates. The two-virulence factor of V. parahaemolyticus, thermostable direct hemolysin (tdh) and TDH-related hemolysin (trh) was screened via PCR. As such, four isolates were trh+and none were tdh+. Majority of the isolates presented high resistance to ampicillin (88%), amikacin (64%), and kanamycin (50%). In addition, this study identified 19-imipenem resistant isolates isolated from freshwater and marine fish samples. Further analysis of these 19-imipenem resistant isolates revealed that the resistance toward imipenem was plasmid mediated after plasmid curing assay. The multiple antibiotics resistance index was >0.2 for 70% of the isolates. In summary, the results confirm the presence of V. parahaemolyticus in freshwater and marine fish samples in Selangor, Malaysia. To our best knowledge, this is the first report discovering the antibiotic resistant patterns and Carbapenem-resistant isolates of V. parahaemolyticus isolated from marine and freshwater fish samples in Selangor.https://www.frontiersin.org/article/10.3389/fmicb.2018.02513/fullVibrio parahaemolyticuscarbapenemfreshwatermarineantibiotic resistantMAR index |
| spellingShingle | Learn-Han Lee Learn-Han Lee Learn-Han Lee Nurul-Syakima Ab Mutalib Jodi Woan-Fei Law Jodi Woan-Fei Law Sunny Hei Wong Vengadesh Letchumanan Vengadesh Letchumanan Discovery on Antibiotic Resistance Patterns of Vibrio parahaemolyticus in Selangor Reveals Carbapenemase Producing Vibrio parahaemolyticus in Marine and Freshwater Fish Frontiers in Microbiology Vibrio parahaemolyticus carbapenem freshwater marine antibiotic resistant MAR index |
| title | Discovery on Antibiotic Resistance Patterns of Vibrio parahaemolyticus in Selangor Reveals Carbapenemase Producing Vibrio parahaemolyticus in Marine and Freshwater Fish |
| title_full | Discovery on Antibiotic Resistance Patterns of Vibrio parahaemolyticus in Selangor Reveals Carbapenemase Producing Vibrio parahaemolyticus in Marine and Freshwater Fish |
| title_fullStr | Discovery on Antibiotic Resistance Patterns of Vibrio parahaemolyticus in Selangor Reveals Carbapenemase Producing Vibrio parahaemolyticus in Marine and Freshwater Fish |
| title_full_unstemmed | Discovery on Antibiotic Resistance Patterns of Vibrio parahaemolyticus in Selangor Reveals Carbapenemase Producing Vibrio parahaemolyticus in Marine and Freshwater Fish |
| title_short | Discovery on Antibiotic Resistance Patterns of Vibrio parahaemolyticus in Selangor Reveals Carbapenemase Producing Vibrio parahaemolyticus in Marine and Freshwater Fish |
| title_sort | discovery on antibiotic resistance patterns of vibrio parahaemolyticus in selangor reveals carbapenemase producing vibrio parahaemolyticus in marine and freshwater fish |
| topic | Vibrio parahaemolyticus carbapenem freshwater marine antibiotic resistant MAR index |
| url | https://www.frontiersin.org/article/10.3389/fmicb.2018.02513/full |
| work_keys_str_mv | AT learnhanlee discoveryonantibioticresistancepatternsofvibrioparahaemolyticusinselangorrevealscarbapenemaseproducingvibrioparahaemolyticusinmarineandfreshwaterfish AT learnhanlee discoveryonantibioticresistancepatternsofvibrioparahaemolyticusinselangorrevealscarbapenemaseproducingvibrioparahaemolyticusinmarineandfreshwaterfish AT learnhanlee discoveryonantibioticresistancepatternsofvibrioparahaemolyticusinselangorrevealscarbapenemaseproducingvibrioparahaemolyticusinmarineandfreshwaterfish AT nurulsyakimaabmutalib discoveryonantibioticresistancepatternsofvibrioparahaemolyticusinselangorrevealscarbapenemaseproducingvibrioparahaemolyticusinmarineandfreshwaterfish AT jodiwoanfeilaw discoveryonantibioticresistancepatternsofvibrioparahaemolyticusinselangorrevealscarbapenemaseproducingvibrioparahaemolyticusinmarineandfreshwaterfish AT jodiwoanfeilaw discoveryonantibioticresistancepatternsofvibrioparahaemolyticusinselangorrevealscarbapenemaseproducingvibrioparahaemolyticusinmarineandfreshwaterfish AT sunnyheiwong discoveryonantibioticresistancepatternsofvibrioparahaemolyticusinselangorrevealscarbapenemaseproducingvibrioparahaemolyticusinmarineandfreshwaterfish AT vengadeshletchumanan discoveryonantibioticresistancepatternsofvibrioparahaemolyticusinselangorrevealscarbapenemaseproducingvibrioparahaemolyticusinmarineandfreshwaterfish AT vengadeshletchumanan discoveryonantibioticresistancepatternsofvibrioparahaemolyticusinselangorrevealscarbapenemaseproducingvibrioparahaemolyticusinmarineandfreshwaterfish |