Bactericidal activity of anti-ABO antibodies in isolated colonies from Escherichia coli
The interaction between ABO antigens and microorganisms, including those present in the microbiota, has been studied about the possible action of antigens and ABO antibodies in susceptibility to infectious diseases. This research aimed to determine the minimum titer of the Escherichia coli bacteria...
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| Format: | Article |
| Language: | Spanish |
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Universidad Nacional de Colombia
2023-06-01
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| Series: | Revista Colombiana de Biotecnología |
| Subjects: | |
| Online Access: | https://revistas.unal.edu.co/index.php/biotecnologia/article/view/100883 |
| _version_ | 1797731280325967872 |
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| author | Nicolli Butzke de Souza Naiara Merenciano Gomes Alexandre Bella Cruz Daniela Valcarenghi Manuela Cozer Alexandre Geraldo |
| author_facet | Nicolli Butzke de Souza Naiara Merenciano Gomes Alexandre Bella Cruz Daniela Valcarenghi Manuela Cozer Alexandre Geraldo |
| author_sort | Nicolli Butzke de Souza |
| collection | DOAJ |
| description |
The interaction between ABO antigens and microorganisms, including those present in the microbiota, has been studied about the possible action of antigens and ABO antibodies in susceptibility to infectious diseases. This research aimed to determine the minimum titer of the Escherichia coli bacteria capable of undergoing in vitro bactericidal action of human anti-ABO antibodies. The selection of blood samples was performed through a questionnaire, blood phenotyping (one volunteer of each ABO phenotype), and the titration of ABO antibodies. A bacterial suspension (inoculum) was prepared and added to the serum of the volunteers, followed by inoculation in Mueller Hinton Agar. After 24 hours, the results were read and interpreted with duplicate analysis. There was no significant difference in the bactericidal test between tests 1 and 2 in blood groups A, B, AB, O, and Positive Control. There was a significant difference in pure human serum when Group A x Positive Control was analyzed, Group B x Positive Control, Group AB x Positive Control, and Group O x Positive Control. There was no significant difference in the other dilutions. It is concluded that anti-ABO antibodies have a bactericidal effect when there is a high concentration of bacteria in the environment.
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| first_indexed | 2024-03-12T11:56:29Z |
| format | Article |
| id | doaj.art-2ee676b7c36b4bd995520ddac5e2bb20 |
| institution | Directory Open Access Journal |
| issn | 0123-3475 1909-8758 |
| language | Spanish |
| last_indexed | 2024-03-12T11:56:29Z |
| publishDate | 2023-06-01 |
| publisher | Universidad Nacional de Colombia |
| record_format | Article |
| series | Revista Colombiana de Biotecnología |
| spelling | doaj.art-2ee676b7c36b4bd995520ddac5e2bb202023-08-30T16:10:14ZspaUniversidad Nacional de ColombiaRevista Colombiana de Biotecnología0123-34751909-87582023-06-0125110.15446/rev.colomb.biote.v25n1.100883Bactericidal activity of anti-ABO antibodies in isolated colonies from Escherichia coliNicolli Butzke de Souza0Naiara Merenciano Gomes1Alexandre Bella Cruz2Daniela Valcarenghi3Manuela Cozer4Alexandre Geraldo5Universidade do Vale do ItajaíUniversidade do Vale do ItajaíUniversidade do Vale do ItajaíUniversidade do Vale do ItajaíUniversidade do Vale do ItajaíCollege of Health Sciences. UNIVALI. Itajaí, 88032-005, Brazil The interaction between ABO antigens and microorganisms, including those present in the microbiota, has been studied about the possible action of antigens and ABO antibodies in susceptibility to infectious diseases. This research aimed to determine the minimum titer of the Escherichia coli bacteria capable of undergoing in vitro bactericidal action of human anti-ABO antibodies. The selection of blood samples was performed through a questionnaire, blood phenotyping (one volunteer of each ABO phenotype), and the titration of ABO antibodies. A bacterial suspension (inoculum) was prepared and added to the serum of the volunteers, followed by inoculation in Mueller Hinton Agar. After 24 hours, the results were read and interpreted with duplicate analysis. There was no significant difference in the bactericidal test between tests 1 and 2 in blood groups A, B, AB, O, and Positive Control. There was a significant difference in pure human serum when Group A x Positive Control was analyzed, Group B x Positive Control, Group AB x Positive Control, and Group O x Positive Control. There was no significant difference in the other dilutions. It is concluded that anti-ABO antibodies have a bactericidal effect when there is a high concentration of bacteria in the environment. https://revistas.unal.edu.co/index.php/biotecnologia/article/view/100883bactericidal capacityanti-ABO antibodiesEscherichia coli |
| spellingShingle | Nicolli Butzke de Souza Naiara Merenciano Gomes Alexandre Bella Cruz Daniela Valcarenghi Manuela Cozer Alexandre Geraldo Bactericidal activity of anti-ABO antibodies in isolated colonies from Escherichia coli Revista Colombiana de Biotecnología bactericidal capacity anti-ABO antibodies Escherichia coli |
| title | Bactericidal activity of anti-ABO antibodies in isolated colonies from Escherichia coli |
| title_full | Bactericidal activity of anti-ABO antibodies in isolated colonies from Escherichia coli |
| title_fullStr | Bactericidal activity of anti-ABO antibodies in isolated colonies from Escherichia coli |
| title_full_unstemmed | Bactericidal activity of anti-ABO antibodies in isolated colonies from Escherichia coli |
| title_short | Bactericidal activity of anti-ABO antibodies in isolated colonies from Escherichia coli |
| title_sort | bactericidal activity of anti abo antibodies in isolated colonies from escherichia coli |
| topic | bactericidal capacity anti-ABO antibodies Escherichia coli |
| url | https://revistas.unal.edu.co/index.php/biotecnologia/article/view/100883 |
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