Colistin Selection of the <i>Mcr-1</i> Gene in Broiler Chicken Intestinal Microbiota
Colistin has a long story of safe use in animals for the treatment and prevention of certain bacterial diseases. Nevertheless, the first description of the <i>mcr-1</i> gene showed that colistin resistance can spread by horizontal gene transfer and changed the landscape. This study aimed...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-06-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/10/6/677 |
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| author | Pedro Miguela-Villoldo Miguel A. Moreno Agustín Rebollada-Merino Antonio Rodríguez-Bertos Marta Hernández David Rodríguez-Lázaro Alejandro Gallardo Alberto Quesada Joaquín Goyache Lucas Domínguez María Ugarte-Ruiz |
| author_facet | Pedro Miguela-Villoldo Miguel A. Moreno Agustín Rebollada-Merino Antonio Rodríguez-Bertos Marta Hernández David Rodríguez-Lázaro Alejandro Gallardo Alberto Quesada Joaquín Goyache Lucas Domínguez María Ugarte-Ruiz |
| author_sort | Pedro Miguela-Villoldo |
| collection | DOAJ |
| description | Colistin has a long story of safe use in animals for the treatment and prevention of certain bacterial diseases. Nevertheless, the first description of the <i>mcr-1</i> gene showed that colistin resistance can spread by horizontal gene transfer and changed the landscape. This study aimed to assess the effect of colistin administration on the dispersion of resistance in the microbiota of day-old broiler chicks and how the presence of <i>mcr-1</i> genes influences the spread of colistin resistance determinants. In this study, 100 one-day-old chicks were divided into four groups of 25 animals (G1, G2, G3, and G4). Animals from G3/G4 were challenged with <i>mcr-1</i>-carrying <i>Salmonella</i> (day 7), while colistin (600 mg/L) was administered daily to G2/G4 animals through drinking water (from day 8 to day 15). Two quantitative PCR assays were performed to compare the amount of <i>Salmonella</i> and <i>mcr-1</i> that were present in the caecal samples. We observed that levels of <i>mcr-1</i> were higher in G3/G4 animals, especially G4, due to the spread of <i>mcr-1</i>-carrying <i>Salmonella</i>. On day 21, <i>Salmonella</i> levels decreased in G4, reaching similar values as those for G3, but <i>mcr-1</i> levels remained significantly higher, suggesting that colistin may accelerate the spreading process when <i>mcr-1</i>-carrying bacteria reach the gut. |
| first_indexed | 2024-03-10T10:40:48Z |
| format | Article |
| id | doaj.art-2ee7a33fbfeb4c36b37e1dff64cd9b3c |
| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-10T10:40:48Z |
| publishDate | 2021-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-2ee7a33fbfeb4c36b37e1dff64cd9b3c2023-11-21T22:55:41ZengMDPI AGAntibiotics2079-63822021-06-0110667710.3390/antibiotics10060677Colistin Selection of the <i>Mcr-1</i> Gene in Broiler Chicken Intestinal MicrobiotaPedro Miguela-Villoldo0Miguel A. Moreno1Agustín Rebollada-Merino2Antonio Rodríguez-Bertos3Marta Hernández4David Rodríguez-Lázaro5Alejandro Gallardo6Alberto Quesada7Joaquín Goyache8Lucas Domínguez9María Ugarte-Ruiz10VISAVET Health Surveillance Centre, Universidad Complutense de Madrid, Avenida Puerta de Hierro, s/n, 28040 Madrid, SpainVISAVET Health Surveillance Centre, Universidad Complutense de Madrid, Avenida Puerta de Hierro, s/n, 28040 Madrid, SpainVISAVET Health Surveillance Centre, Universidad Complutense de Madrid, Avenida Puerta de Hierro, s/n, 28040 Madrid, SpainVISAVET Health Surveillance Centre, Universidad Complutense de Madrid, Avenida Puerta de Hierro, s/n, 28040 Madrid, SpainLaboratorio de Biología Molecular y Microbiología, Instituto Tecnológico Agrario de Castilla y León, Ctra. Burgos Km. 119, 47071 Valladolid, SpainÁrea de Microbiología, Departamento de Biotecnología y Ciencia de los Alimentos, Facultad de Ciencias, Universidad de Burgos, Plaza Misael Bañuelos s/n, 09001 Burgos, SpainDepartamento de Bioquímica, Biología Molecular y Genética, Facultad de Veterinaria, Universidad de Extremadura, Avda. de la Universidad s/n, 10003 Caceres, SpainDepartamento de Bioquímica, Biología Molecular y Genética, Facultad de Veterinaria, Universidad de Extremadura, Avda. de la Universidad s/n, 10003 Caceres, SpainVISAVET Health Surveillance Centre, Universidad Complutense de Madrid, Avenida Puerta de Hierro, s/n, 28040 Madrid, SpainVISAVET Health Surveillance Centre, Universidad Complutense de Madrid, Avenida Puerta de Hierro, s/n, 28040 Madrid, SpainVISAVET Health Surveillance Centre, Universidad Complutense de Madrid, Avenida Puerta de Hierro, s/n, 28040 Madrid, SpainColistin has a long story of safe use in animals for the treatment and prevention of certain bacterial diseases. Nevertheless, the first description of the <i>mcr-1</i> gene showed that colistin resistance can spread by horizontal gene transfer and changed the landscape. This study aimed to assess the effect of colistin administration on the dispersion of resistance in the microbiota of day-old broiler chicks and how the presence of <i>mcr-1</i> genes influences the spread of colistin resistance determinants. In this study, 100 one-day-old chicks were divided into four groups of 25 animals (G1, G2, G3, and G4). Animals from G3/G4 were challenged with <i>mcr-1</i>-carrying <i>Salmonella</i> (day 7), while colistin (600 mg/L) was administered daily to G2/G4 animals through drinking water (from day 8 to day 15). Two quantitative PCR assays were performed to compare the amount of <i>Salmonella</i> and <i>mcr-1</i> that were present in the caecal samples. We observed that levels of <i>mcr-1</i> were higher in G3/G4 animals, especially G4, due to the spread of <i>mcr-1</i>-carrying <i>Salmonella</i>. On day 21, <i>Salmonella</i> levels decreased in G4, reaching similar values as those for G3, but <i>mcr-1</i> levels remained significantly higher, suggesting that colistin may accelerate the spreading process when <i>mcr-1</i>-carrying bacteria reach the gut.https://www.mdpi.com/2079-6382/10/6/677colistin<i>mcr-1</i><i>Salmonella</i>in vivo assayreal-time PCR quantificationmicrobiota |
| spellingShingle | Pedro Miguela-Villoldo Miguel A. Moreno Agustín Rebollada-Merino Antonio Rodríguez-Bertos Marta Hernández David Rodríguez-Lázaro Alejandro Gallardo Alberto Quesada Joaquín Goyache Lucas Domínguez María Ugarte-Ruiz Colistin Selection of the <i>Mcr-1</i> Gene in Broiler Chicken Intestinal Microbiota Antibiotics colistin <i>mcr-1</i> <i>Salmonella</i> in vivo assay real-time PCR quantification microbiota |
| title | Colistin Selection of the <i>Mcr-1</i> Gene in Broiler Chicken Intestinal Microbiota |
| title_full | Colistin Selection of the <i>Mcr-1</i> Gene in Broiler Chicken Intestinal Microbiota |
| title_fullStr | Colistin Selection of the <i>Mcr-1</i> Gene in Broiler Chicken Intestinal Microbiota |
| title_full_unstemmed | Colistin Selection of the <i>Mcr-1</i> Gene in Broiler Chicken Intestinal Microbiota |
| title_short | Colistin Selection of the <i>Mcr-1</i> Gene in Broiler Chicken Intestinal Microbiota |
| title_sort | colistin selection of the i mcr 1 i gene in broiler chicken intestinal microbiota |
| topic | colistin <i>mcr-1</i> <i>Salmonella</i> in vivo assay real-time PCR quantification microbiota |
| url | https://www.mdpi.com/2079-6382/10/6/677 |
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