| Summary: | As new techniques for single-cell analysis are evolving, there is a growing need for streamlined and flexible protocols for single-cell suspension preparation and single-cell isolation. Based on previous research on the model species zebrafish (<i>Danio rerio</i>), a novel protocol for the isolation of chromatophores from brown trout (<i>Salmo trutta</i>) skin was developed and specific gene expression in chromatophore types evaluated with qPCR. This is the first report on a protocol for the isolation of chromatophores from the skin of salmonids. The method is simple and fast (taking about 90 min), requires no special equipment and is in line with the high ethical standards required in animal research, as no animals need to be sacrificed. In addition, skin biopsies can be taken from the same fish at multiple time points, allowing monitoring of chromatophore differentiation and their involvement in the formation of pigmentation patterns. The protocol should be effective for the vast majority of salmonid species due to similarities in skin morphology.
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