Love Wave Immunosensor for the Detection of Carbaryl Pesticide
A Love Wave (LW) immunosensor was developed for the detection of carbaryl pesticide. The experimental setup consisted on: a compact electronic characterization circuit based on phase and amplitude detection at constant frequency; an automated flow injection system; a thermal control unit; a custom-m...
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| Format: | Article |
| Language: | English |
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MDPI AG
2014-09-01
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| Series: | Sensors |
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| Online Access: | http://www.mdpi.com/1424-8220/14/9/16434 |
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| author | María-Isabel Rocha-Gaso José-Vicente García Pablo García Carmen March-Iborra Yolanda Jiménez Laurent-Alain Francis Ángel Montoya Antonio Arnau |
| author_facet | María-Isabel Rocha-Gaso José-Vicente García Pablo García Carmen March-Iborra Yolanda Jiménez Laurent-Alain Francis Ángel Montoya Antonio Arnau |
| author_sort | María-Isabel Rocha-Gaso |
| collection | DOAJ |
| description | A Love Wave (LW) immunosensor was developed for the detection of carbaryl pesticide. The experimental setup consisted on: a compact electronic characterization circuit based on phase and amplitude detection at constant frequency; an automated flow injection system; a thermal control unit; a custom-made flow-through cell; and Quartz /SiO2 LW sensors with a 40 μm wavelength and 120 MHz center frequency. The carbaryl detection was based on a competitive immunoassay format using LIB-CNH45 monoclonal antibody (MAb). Bovine Serum Albumin-CNH (BSA-CNH) carbaryl hapten-conjugate was covalently immobilized, via mercaptohexadecanoic acid self-assembled monolayer (SAM), onto the gold sensing area of the LW sensors. This immobilization allowed the reusability of the sensor for at least 70 assays without significant signal losses. The LW immunosensor showed a limit of detection (LOD) of 0.09 μg/L, a sensitivity of 0.31 μg/L and a linear working range of 0.14–1.63 μg/L. In comparison to other carbaryl immunosensors, the LW immunosensor achieved a high sensitivity and a low LOD. These features turn the LW immunosensor into a promising tool for applications that demand a high resolution, such as for the detection of pesticides in drinking water at European regulatory levels. |
| first_indexed | 2024-04-14T03:33:36Z |
| format | Article |
| id | doaj.art-2eed16436ee949458c068cf72352bcb0 |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-04-14T03:33:36Z |
| publishDate | 2014-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-2eed16436ee949458c068cf72352bcb02022-12-22T02:14:51ZengMDPI AGSensors1424-82202014-09-01149164341645310.3390/s140916434s140916434Love Wave Immunosensor for the Detection of Carbaryl PesticideMaría-Isabel Rocha-Gaso0José-Vicente García1Pablo García2Carmen March-Iborra3Yolanda Jiménez4Laurent-Alain Francis5Ángel Montoya6Antonio Arnau7Grupo de Fenómenos Ondulatorios (GFO), Universitat Politècnica de València, 46022 Valencia, SpainGrupo de Fenómenos Ondulatorios (GFO), Universitat Politècnica de València, 46022 Valencia, SpainGrupo de Fenómenos Ondulatorios (GFO), Universitat Politècnica de València, 46022 Valencia, SpainInstituto Interuniversitario de Investigación en Bioingeniería y Tecnología Orientada al Ser Humano (I3BH), Universitat Politècnica de València, 46022 Valencia, SpainGrupo de Fenómenos Ondulatorios (GFO), Universitat Politècnica de València, 46022 Valencia, SpainSensors, Microsystems and Actuators Laboratory of Louvain (SMALL), ICTEAM Institute, Université Catholique de Louvain, 1348 Louvain-la-Neuve, BelgiumInstituto Interuniversitario de Investigación en Bioingeniería y Tecnología Orientada al Ser Humano (I3BH), Universitat Politècnica de València, 46022 Valencia, SpainGrupo de Fenómenos Ondulatorios (GFO), Universitat Politècnica de València, 46022 Valencia, SpainA Love Wave (LW) immunosensor was developed for the detection of carbaryl pesticide. The experimental setup consisted on: a compact electronic characterization circuit based on phase and amplitude detection at constant frequency; an automated flow injection system; a thermal control unit; a custom-made flow-through cell; and Quartz /SiO2 LW sensors with a 40 μm wavelength and 120 MHz center frequency. The carbaryl detection was based on a competitive immunoassay format using LIB-CNH45 monoclonal antibody (MAb). Bovine Serum Albumin-CNH (BSA-CNH) carbaryl hapten-conjugate was covalently immobilized, via mercaptohexadecanoic acid self-assembled monolayer (SAM), onto the gold sensing area of the LW sensors. This immobilization allowed the reusability of the sensor for at least 70 assays without significant signal losses. The LW immunosensor showed a limit of detection (LOD) of 0.09 μg/L, a sensitivity of 0.31 μg/L and a linear working range of 0.14–1.63 μg/L. In comparison to other carbaryl immunosensors, the LW immunosensor achieved a high sensitivity and a low LOD. These features turn the LW immunosensor into a promising tool for applications that demand a high resolution, such as for the detection of pesticides in drinking water at European regulatory levels.http://www.mdpi.com/1424-8220/14/9/16434biosensorcarbaryl pesticide detectionimmunosensorLove Wave sensorSurface Acoustic Wave (SAW) |
| spellingShingle | María-Isabel Rocha-Gaso José-Vicente García Pablo García Carmen March-Iborra Yolanda Jiménez Laurent-Alain Francis Ángel Montoya Antonio Arnau Love Wave Immunosensor for the Detection of Carbaryl Pesticide Sensors biosensor carbaryl pesticide detection immunosensor Love Wave sensor Surface Acoustic Wave (SAW) |
| title | Love Wave Immunosensor for the Detection of Carbaryl Pesticide |
| title_full | Love Wave Immunosensor for the Detection of Carbaryl Pesticide |
| title_fullStr | Love Wave Immunosensor for the Detection of Carbaryl Pesticide |
| title_full_unstemmed | Love Wave Immunosensor for the Detection of Carbaryl Pesticide |
| title_short | Love Wave Immunosensor for the Detection of Carbaryl Pesticide |
| title_sort | love wave immunosensor for the detection of carbaryl pesticide |
| topic | biosensor carbaryl pesticide detection immunosensor Love Wave sensor Surface Acoustic Wave (SAW) |
| url | http://www.mdpi.com/1424-8220/14/9/16434 |
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